Metabolomic profiling of metoprolol-induced cardioprotection in a murine model of acute myocardial ischemia

Lai, Qiong; Yuan, Gao; Wang, Hao; Liu, Zeliang; Kou, Junping; Yu, Boyang; Li, Fang

doi:10.1016/j.biopha.2020.109820

Cited by 12 publications

(11 citation statements)

References 48 publications

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“…tissues, metabolomics reveals metabolic patterns and provides insight into the mechanisms underlying physiological conditions and diseases [5]. Considerable numbers of metabolomic studies have been conducted on cardiovascular diseases [6].…”

Section: Volcano Plot and Heatmaps Of Differentially Expressed Metabolitesmentioning

confidence: 99%

“…A recent study determined that levels of purine and pyrimidine metabolites were altered in patients with myocardial injury caused by anticancer drugs [44]. Further, levels of purine and pyrimidine metabolites reportedly changed in mice with acute myocardial ischemia, indicating that purine and pyrimidine metabolism disorders may be associated with myocardial injury [5]. Previous metabolomics studies have reported that alcohol exposure changed the level of hypoxanthine, but different studies have reported different trends [45][46][47].…”

Section: Metabolism Of Pyrimidines and Purinesmentioning

confidence: 99%

“…Metabolomics is a new method of studying biological systems developed after genomics and proteomics. By analyzing small molecular substances in biological fluids and tissues, metabolomics reveals metabolic patterns and provides insight into the mechanisms underlying physiological conditions and diseases [5]. Considerable numbers of metabolomic studies have been conducted on cardiovascular diseases [6].…”

Section: Introductionmentioning

confidence: 99%

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A Pilot Metabolomic Study on Myocardial Injury Caused by Chronic Alcohol Consumption—Alcoholic Cardiomyopathy

et al. 2021

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Chronic alcohol consumption leads to myocardial injury, ventricle dilation, and cardiac dysfunction, which is defined as alcoholic cardiomyopathy (ACM). To explore the induced myocardial injury and underlying mechanism of ACM, the Liber-DeCarli liquid diet was used to establish an animal model of ACM and histopathology, echocardiography, molecular biology, and metabolomics were employed. Hematoxylin-eosin and Masson’s trichrome staining revealed disordered myocardial structure and local fibrosis in the ACM group. Echocardiography revealed thinning wall and dilation of the left ventricle and decreased cardiac function in the ACM group, with increased serum levels of brain natriuretic peptide (BNP) and expression of myocardial BNP mRNA measured through enzyme-linked immunosorbent assay and real-time quantitative polymerase chain reaction (PCR), respectively. Through metabolomic analysis of myocardium specimens, 297 differentially expressed metabolites were identified which were involved in KEGG pathways related to the biosynthesis of unsaturated fatty acids, vitamin digestion and absorption, oxidative phosphorylation, pentose phosphate, and purine and pyrimidine metabolism. The present study demonstrated chronic alcohol consumption caused disordered cardiomyocyte structure, thinning and dilation of the left ventricle, and decreased cardiac function. Metabolomic analysis of myocardium specimens and KEGG enrichment analysis further demonstrated that several differentially expressed metabolites and pathways were involved in the ACM group, which suggests potential causes of myocardial injury due to chronic alcohol exposure and provides insight for further research elucidating the underlying mechanisms of ACM.

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Section: Volcano Plot and Heatmaps Of Differentially Expressed Metabolitesmentioning

confidence: 99%

Section: Metabolism Of Pyrimidines and Purinesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Pilot Metabolomic Study on Myocardial Injury Caused by Chronic Alcohol Consumption—Alcoholic Cardiomyopathy

et al. 2021

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“…LDH and CK are important indicators for judging myocardial injury, and their expression in normal myocardial tissue is low. When the body is subjected to a stress response or cardiac ischemia, LDH and CK in the blood increase significantly [ 12 ]. Therefore, in order to determine the success rate of modeling, we also detected the activity of LDH and CK in the serum of AMI rats.…”

Section: Discussionmentioning

confidence: 99%

Death-Associated Protein Kinase 1 (DAPK1) Protects against Myocardial Injury Induced by Myocardial Infarction in Rats via Inhibition of Inflammation and Oxidative Stress

Zhang¹,

Zhang²,

Zhou³

et al. 2022

Disease Markers

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Objective. Heart failure and ventricular remodeling after acute myocardial infarction (AMI) are important factors affecting the prognosis of patients. Therefore, we expected to explore the therapeutic target of AMI by studying the effect of death-associated protein kinase 1 (DAPK1) on AMI rat model. Materials and Methods. We used male Sprague-Dawley rats to make AMI model, and after 1, 3, 7, and 14 d, we detect the success rate of modeling and the expression change of DAPK1 through 2, 3, 5-triphenyl tetrazolium chloride staining, myocardial injury markers detection, echocardiographic detection, and histological experiment. In addition, we determined the effect of DAPK1 on AMI by subcutaneous injection of the DAPK1 inhibitor (TC-DAPK 6). The effect of DAPK1 on cardiomyocytes has also been verified in cell experiments on H9c2 cells. Results. The expression of DAPK1 in AMI rats was significantly higher than that in sham group, and it increased with time. The expression of inflammatory factors (interleukin- (IL-) 1β, IL-6, and tumor necrosis factor-α) in AMI rats treated by TC-DAPK 6 was reduced. In addition, TC-DAPK 6 also reduced the activity of malonaldehyde and increased the activities of superoxide dismutase, glutathione, and catalase. The expression of antioxidant molecules such as peroxiredoxin1/4 and glutathione peroxidase1/3 was also promoted by TC-DAPK 6. In H9c2 cells, TC-DAPK 6 also reduced its oxidative stress level. Conclusions. The increase of DAPK1 may be related to the pathogenesis of AMI. DAPK1 inhibitors protect cardiomyocytes from AMI-induced myocardial injury by reducing levels of inflammation and oxidative stress in myocardial tissue and cells.

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“…Till date there is scarcity of studies to understand the metabolomic changes in blood plasma of RHD patients. Metabolomics is a new approach after genomics and proteomics, which is being used extensively to identify disease biomarkers and biological systems 4 . Metabolomics approach has been used in detecting biomarkers in diseases like cancer, neurological disorders, infectious diseases, inflammation and also in cardiovascular diseases 5 .…”

Section: Discussionmentioning

confidence: 99%

An Untargeted LC–MS based approach for identification of altered metabolites in blood plasma of rheumatic heart disease patients

Das

Kumar

Sharma

et al. 2022

Sci Rep

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Rheumatic heart disease (RHD) is often considered as a disease of developing countries and India is the home of about 40% of RHD patients. Environment seems to play a major role in its causation. Since gene environment interactions can lead to alterations of various metabolic pathways, identification of altered metabolites can help in understanding the various pathways leading to RHD. Blood plasma samples from 51 RHD and 49 healthy controls were collected for the study. Untargeted metabolomics approach was used to identify the metabolites that are altered in RHD patients. Data showed 25 altered metabolites among RHD patients. These altered metabolites were those involved in Purine, Glutamine, Glutamate, Pyrimidine, Arginine, Proline and Linoleic metabolism. Thus, the present study illuminates metabolic alterations among RHD patients which can help in determining the potential therapeutic targets.

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Metabolomic profiling of metoprolol-induced cardioprotection in a murine model of acute myocardial ischemia

Cited by 12 publications

References 48 publications

A Pilot Metabolomic Study on Myocardial Injury Caused by Chronic Alcohol Consumption—Alcoholic Cardiomyopathy

A Pilot Metabolomic Study on Myocardial Injury Caused by Chronic Alcohol Consumption—Alcoholic Cardiomyopathy

Death-Associated Protein Kinase 1 (DAPK1) Protects against Myocardial Injury Induced by Myocardial Infarction in Rats via Inhibition of Inflammation and Oxidative Stress

An Untargeted LC–MS based approach for identification of altered metabolites in blood plasma of rheumatic heart disease patients

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