Metabolomic Profiling Reveals the Difference on Reproductive Performance between High and Low Lactational Weight Loss Sows

Hu, Liang; Che, Lianqiang; Wu, Chen Ten; Curtasu, Mihai Victor; Wu, Fali; Fang, Zhengfeng; Lin, Yan; Xu, Shengyu; Feng, Bin; Li, Jian; Zhuo, Yong; Theil, Peter Kappel; Wu, De

doi:10.3390/metabo9120295

Cited by 16 publications

(12 citation statements)

References 62 publications

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“…The samples were analyzed using a triple time-of-flight (TOF) mass spectrometer (ESI/Triple TOF 5600; AB Sciex, Concord, Canada) equipped with an electrospray ionization source used in positive and negative ion modes. The pretreatment, extraction, and identification of serum samples were according to the procedure described by Hu et al [ 27 ]. The raw data (whiff scan files) were converted into mzXML format using ProteoWizard [ 28 ] and were imported to the XCMS software for peak matching, retention time alignment, and peak area extraction [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota

Zhou

Sun

et al. 2021

J Animal Sci Biotechnol

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Background Previous studies have shown that exogenous short-chain fatty acids (SCFAs) introduction attenuated the body fat deposition in conventional mice and pigs. However, limited studies have evaluated the effects of exogenously introduced SCFAs on the lipid and glucose metabolism independently of the gut microbiota. This study was to investigate the effects of exogenous introduction of SCFAs on the lipid and glucose metabolism in a germ-free (GF) pig model. Methods Twelve hysterectomy-derived newborn pigs were reared in six sterile isolators. All pigs were hand-fed with sterile milk powder for 21 d, then the sterile feed was introduced to pigs for another 21 d. In the second 21-d period, six pigs were orally administrated with 25 mL/kg sterile saline per day and considered as the GF group, while the other six pigs were orally administrated with 25 mL/kg SCFAs mixture (acetic, propionic, and butyric acids, 45, 15, and 11 mmol/L, respectively) per day and regarded as FA group. Results Orally administrated with SCFAs tended to increase the adiponectin concentration in serum, enhance the CPT-1 activity in longissimus dorsi, and upregulate the ANGPTL4 mRNA expression level in colon (P < 0.10). Meanwhile, the mRNA abundances of ACC, FAS, and SREBP-1C in liver and CD36 in longissimus dorsi of the FA group were decreased (P < 0.05) compared with those in the GF group. Besides, the mRNA expression of PGC-1α in liver and LPL in longissimus dorsi tended to (P < 0.10) upregulate and downregulate respectively in the FA group. Moreover, oral administration of SCFAs tended to increase the protein level of GPR43 (P < 0.10) and decrease the protein level of ACC (P < 0.10) in liver. Also, oral administration of SCFAs upregulated the p-AMPK/AMPK ratio and the mRNA expressions of GLUT-2 and GYS2 in liver (P < 0.05). In addition, the metabolic pathway associated with the biosynthesis of unsaturated fatty acids was most significantly promoted (P < 0.05) by oral administration of SCFAs. Conclusions Exogenous introduction of SCFAs might attenuate the fat deposition and to some extent improve the glucose control in the pig model, which occurred independently of the gut microbiota.

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Section: Methodsmentioning

confidence: 99%

Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota

Zhou

Sun

et al. 2021

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…The samples were analyzed using a triple time-of-ight (TOF) mass spectrometer (ESI/Triple TOF 5600; AB Sciex, Concord, Canada) equipped with an electrospray ionization source used in positive and negative ion modes. The pretreatment, extraction, and identi cation of serum samples were according to the procedure described by Hu et al ( 2019) [27]. The raw data (whiff scan les) were converted into mzXML format using ProteoWizard [28] and were imported to the XCMS software for peak matching, retention time alignment, and peak area extraction [29].…”

Section: Determination Of Protein Levelsmentioning

confidence: 99%

Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota

Zhang¹,

Yu²,

Sun

et al. 2021

Preprint

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Background: Previous studies have shown that exogenous short-chain fatty acids (SCFAs) introduction attenuated the body fat deposition in conventional mice and pigs. However, limited studies have evaluated the effects of exogenously introduced SCFAs on the lipid and glucose metabolism independently of the gut microbiota. This study was to investigate the effects of exogenous introduction of SCFAs on the lipid and glucose metabolism in a germ-free (GF) pig model. Methods: Twelve hysterectomy-derived newborn pigs were reared in six sterile isolators. All pigs were hand-fed with sterile milk powder for 21 d, then the sterile feed was introduced to pigs for another 21 d. In the second 21-d period, six pigs were orally administrated with 25 mL/kg sterile saline per day and considered as the GF group, while the other six pigs were orally administrated with 25 mL/kg SCFAs mixture (acetic, propionic, and butyric acids, 45, 15, and 11 mmol/L, respectively) per day and regarded as FA group.Results: Orally administrated with SCFAs tended to increase the adiponectin concentration in serum, enhance the CPT-1 activity in longissimus dorsi, and upregulate the ANGPTL4 mRNA expression level in colon (P < 0.10). Meanwhile, the mRNA abundances of ACC, FAS, and SREBP-1C in liver and CD36 in longissimus dorsi of the FA group were decreased (P < 0.05) compared with those in the GF group. Besides, the mRNA expression of PGC-1α in liver and LPL in longissimus dorsi tended to (P < 0.10) upregulate and downregulate respectively in the FA group. Moreover, oral administration of SCFAs tended to increase the protein level of GPR43 (P < 0.10) and decrease the protein level of ACC (P < 0.10) in liver. Also, oral administration of SCFAs upregulated the p-AMPK/AMPK ratio and the mRNA expressions of GLUT-2 and GYS2 in liver (P < 0.05). In addition, the metabolic pathway associated with the biosynthesis of unsaturated fatty acids was most significantly promoted (P < 0.05) by oral administration of SCFAs.Conclusions: Exogenous introduction of SCFAs might attenuate the fat deposition and to some extent improve the glucose control in the pig model, which occurred independently of the gut microbiota.

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“…This study found that sows supplemented with FFM had less BF and BW loss compared with those in the CON group. Additionally, the sows that lost excessive amounts of BW had extended weaning-to-estrous intervals (WEIs) [ 45 ]. The tendency for prolonged WEIs in the FFM group in this study suggests that the amount of fiber added may have been close to the maximum a sow can tolerate.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Inclusion of Fermented Mulberry Leaves and Branches in the Gestational Diet on the Performance and Gut Microbiota of Sows and Their Offspring

et al. 2021

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Fermented feed mulberry (FFM), being rich in dietary fiber, has not been fully evaluated to be used in sow’s diet. In this study, we investigated the effects of 25.5% FFM supplemented gestation diets on the performance and gut microbiota of sows and their offspring. Results showed that the serum concentration of glucose, progesterone, and estradiol were not affected by the dietary treatment, while the level of serum insulin and fecal short chain fatty acid were both reduced in FFM group on gestation day 60 (G60, p < 0.05). Additionally, FFM increased both voluntary feed intake and weaning litter weight (p < 0.05), while decreased the losses of both Backfat thickness and bodyweight throughout lactation (p < 0.05). 16S rRNA sequencing showed FFM supplementation significantly increased the diversity and relative abundance of sows’ fecal microbiota on G60 (p < 0.05). The differential microbiota for sows from FFM group was that Bacteroidetes was increased on G60 while Firmicutes were decreased on L7 (p < 0.05), and which for the FFM piglets was that both unclassified_f_Lachnospiraceae on G60 and norank_f_Ruminococcaceae were both increased (p < 0.05). In short, FFM can be recognized as a potential feed ingredient used in sow’s diet.

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Metabolomic Profiling Reveals the Difference on Reproductive Performance between High and Low Lactational Weight Loss Sows

Cited by 16 publications

References 62 publications

Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota

Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota

Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota

Effects of the Inclusion of Fermented Mulberry Leaves and Branches in the Gestational Diet on the Performance and Gut Microbiota of Sows and Their Offspring

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