Metabolomic response of microalgae towards diclofenac sodium during its removal from water and concomitant recovery of pigments and lipids

Sharma, Jyoti; Mariam, Iqra; Kareya, Mukul Suresh; Jutur, Pannaga P.; Joshi, Monika; Harish,; Bhatnagar, Amit; Chaurasia, Akhilesh Kumar; Nigam, Subhasha

doi:10.1016/j.biortech.2023.128617

Cited by 24 publications

(4 citation statements)

References 47 publications

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“…This is consistent with the previous results where DCF alone showed a concentration-wise increase in ROS and MDA levels in Chlorella sorokiniana . 39 Similar to our study, pristine NPs showed an increase in ROS and MDA content on S. obliquus . 24 In the combinations with the higher DCF concentration, an enhancement in ROS and MDA generation was noticed.…”

Section: Discussionsupporting

confidence: 89%

“…can have an inductive effect on the growth of Chlorella sorokiniana , Spirulina platensis , and diatom Navicula sp. 39,40 This biphasic dose response has been noted from primary producers like S. obliquus to higher organisms when exposed to NPs. 41 Similar to our study, the combined effect of PS particles and DBP on Chlorella pyrenoidosa was antagonistic at lower doses of DBP while being synergistic at higher doses.…”

Section: Discussionmentioning

confidence: 89%

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Polystyrene nanoplastics alter the ecotoxicological effects of diclofenac on freshwater microalgae Scenedesmus obliquus

Christudoss,

Chandrasekaran,

Mukherjee

2024

Environ. Sci.: Processes Impacts

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 89%

Polystyrene nanoplastics alter the ecotoxicological effects of diclofenac on freshwater microalgae Scenedesmus obliquus

Christudoss,

Chandrasekaran,

Mukherjee

2024

Environ. Sci.: Processes Impacts

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“…The obtained pellet was separated and resuspended in 90 % methanol, and incubated for 10 min at 55 °C followed by centrifugation to obtain extracted photosynthetic content. Absorbance of the supernatant was measured at 665 nm and 652 nm by UV‐spectrophotometer [64] . Chlorophyll a concentration was evaluated by the following equation (Eq.…”

Section: Methodsmentioning

confidence: 99%

“…Absorbance of the supernatant was measured at 665 nm and 652 nm by UV-spectrophotometer. [64] Chlorophyll a concentration was evaluated by the following equation (Eq. 9):…”

Section: Adsorption Isothermsmentioning

confidence: 99%

Nitrogen‐Doped Graphene Nanosheets for Sustainable Removal of Pharmaceutical Contaminants from Water: Kinetics and ANFIS Modeling

et al. 2023

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In this work, nitrogen‐doped graphene nanosheets (NGS) were synthesized to remove diclofenac sodium (DFS) from water. The NGS exhibited 99.8 % removal efficiency, adsorption capacity of 278 mg g−1 for DFS within 30 min, and excellent reusability up to 5 cycles of adsorption. The experimental data fit well with pseudo‐second order kinetic model and Langmuir isotherm model. X‐ray photoelectron spectroscopy (XPS) and FTIR studies revealed that the carboxylic group, triazine group, graphitic‐N, and pyridinic‐N groups were involved in the adsorption of DFS. The adaptive neuro‐fuzzy inference system (ANFIS) was modeled for accurate prediction of optimizing parameters for the maximum adsorptive removal (%) of DFS by NGS. A subsequent cytotoxicity assay of NGS on a green alga Chlamydomonas sp. signified non‐toxic nature. This study suggests NGS to be a promising economic adsorbent for water remediation in resource‐poor environments to fulfill “UN‐Sustainable Development Goal‐ 6”.

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Diclofenac Degradation by Immobilized Chlamydomonas reinhardtii and Scenedesmus obliquus

Kariyawasam,

Petkovich,

Vriens

2024

MicrobiologyOpen

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Diclofenac (DCF), a commonly used anti‐inflammatory medication, presents environmental concerns due to its presence in water bodies, resistance to conventional wastewater treatment methods, and detection at increasing concentrations (ng/L to µg/L) that highlight DCF as a global emerging pollutant. While microalgae have been effective in degrading DCF in wastewater, immobilization into a matrix offers a promising approach to enhance treatment retention and efficiency. This study aimed to evaluate the efficacy of DCF removal using immobilized freshwater microalgae. Two algal species, Chlamydomonas reinhardtii (Chlamydomonas) and Scenedesmus obliquus (Scenedesmus), were tested for 6 days in both free and immobilized forms to determine if immobilized algae could degrade DCF comparably to free cells. The findings indicate that by Day 3, immobilized Chlamydomonas and Scenedesmus removed 78.0% and 80.1% of DCF, outperforming free‐cell cultures. Mixed cultures demonstrated synergistic effects, with removal amounts of 91.4% for free and 92.3% for immobilized systems. By Day 6, all conditions achieved complete DCF removal (100%). Mechanistic analysis showed 80.0% biodegradation and 20.0% bioaccumulation in free Chlamydomonas and 56.8% biodegradation with 43.2% bioaccumulation in Scenedesmus. Immobilization shifted pathways slightly: in Chlamydomonas, 61.6% of DCF removal occurred via biodegradation, 18.3% via bioaccumulation, and 20.1% via abiotic degradation. For Scenedesmus, immobilization achieved 45.6% biodegradation, 36.6% bioaccumulation, and 17.8% abiotic degradation, enhancing abiotic degradation while maintaining biodegradation efficiency. This research serves as a proof of concept for utilizing immobilized algae in DCF removal and suggests an avenue for improved wastewater treatment of emerging contaminants.

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Metabolomic response of microalgae towards diclofenac sodium during its removal from water and concomitant recovery of pigments and lipids

Cited by 24 publications

References 47 publications

Polystyrene nanoplastics alter the ecotoxicological effects of diclofenac on freshwater microalgae Scenedesmus obliquus

Polystyrene nanoplastics alter the ecotoxicological effects of diclofenac on freshwater microalgae Scenedesmus obliquus

Nitrogen‐Doped Graphene Nanosheets for Sustainable Removal of Pharmaceutical Contaminants from Water: Kinetics and ANFIS Modeling

Diclofenac Degradation by Immobilized Chlamydomonas reinhardtii and Scenedesmus obliquus

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