Metabolomic Status of The Oral Cavity in Chronic Periodontitis

Gawron, Katarzyna; Wojtowicz, Wojciech; Łazarz‐Bartyzel, Katarzyna; Lamasz, A.; Qasem, Badr; Mydel, Piotr; Chomyszyn‐Gajewska, Maria; Potempa, Jan; Młynarz, Piotr

doi:10.21873/invivo.11587

Cited by 34 publications

(29 citation statements)

References 59 publications

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“…As anticipated, DL1 dominance was attenuated in an isogenic spxB -deficient mutant (Δ spxB ), which only displayed dominance against the Δ lrgAB strain, reinforcing a role for LrgAB in S. mutans’ competition with H 2 O 2 -generating S. gordonii . Given that pyruvate is excreted as an overflow metabolite, it should be a common carbon source in microbiome environments such as the oral cavity ( Takahashi et al, 2010 ; Gawron et al, 2019 ). More interestingly, external pyruvate modulated this interspecies competition, with 10 mM pyruvate conferring a great competitive advantage to S. mutans wildtype and SAB161 but not Δ lrgAB.…”

Section: Discussionmentioning

confidence: 99%

Understanding LrgAB Regulation of Streptococcus mutans Metabolism

et al. 2020

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Lack of LrgAB renders cariogenic Streptococcus mutans more sensitive to oxidative stress, as well as limits the capacity of this organism to re-uptake pyruvate upon starvation. This study was aimed at investigating the ecological and metabolic contribution of LrgAB to competitive fitness, using S. mutans strains, that either lack or overexpress lrgAB. These experiments revealed that impaired aerobic growth of the lrgAB mutant can be effectively restored by supplementation of pyruvate, and that perturbated expression of lrgAB significantly affects pyruvate flux and the conversion of pyruvate to acetyl-CoA by the Pdh pathway, verifying that LrgAB is closely linked to pyruvate catabolism. In vitro competition assays revealed that LrgAB plays an important role in S. mutans competition with H 2 O 2-producing S. gordonii, an interaction which can also be modulated by external pyruvate. However, no obvious competitive disadvantage was observed against S. gordonii by either the S. mutans lrgAB mutant or lrgAB overexpression strain in vivo using a mouse caries model. Organic acid analysis of mouse dental biofilms revealed that metabolites produced by the host and/or dental plaque microbiota could complement the deficiency of a lrgAB mutant, and favored S. mutans establishment compared to S. gordonii. Collectively, these results reinforce the importance of the oral microbiota and the metabolic environment in the oral cavity battleground, and highlight that pyruvate uptake through LrgAB may be crucial for interspecies competition that drives niche occupancy.

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Section: Discussionmentioning

confidence: 99%

Understanding LrgAB Regulation of Streptococcus mutans Metabolism

et al. 2020

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“…Such roles of pyruvate may be especially important to S. mutans, given its ability to withstand the limited resources and environmental fluctuations experienced in the oral cavity, and during pathogenic biofilm development on the tooth surface. Pyruvate is excreted as an overflow metabolite (Paczia et al, 2012), thus a common nutrient in microbiome environments such as the oral cavity (Takahashi et al, 2010;Gawron et al, 2019). When bacterial cells experience nutrient limitation (during the transition to stationary phase), they rapidly initiate re-uptake of previously excreted pyruvate through LrgAB (Paczia et al, 2012;Ahn et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Acetate and Potassium Modulate the Stationary-Phase Activation of lrgAB in Streptococcus mutans

et al. 2020

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Frontiers in Microbiology | www.frontiersin.org 1 March 2020 | Volume 11 | Article 401 Ahn et al. Environmental Triggers of lrgAB Expression 600 ) was measured at 37 • C at 30 min-intervals using a Bioscreen C growth curve analysis system. At least three independent experiments, each in quadruplicate, wereFrontiers in Microbiology | www.frontiersin.org absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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“…For this reason, multivariate statistical analysis (MVSA) became very popular to identify set of analytes that can be considered biomarkers of different pathologies [126][127][128]135]. The MVSA can be divided into two distinct categories: the unsupervised analysis, typically performed after the normalization of the data obtained; and supervised analysis, involving a deeper characterization of the relationship between variables (analytes) and cases (sample) [36,49,128,136,137].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, the authors also detected low pyruvate and n-acetyl groups concentrations in same study cohort. Several other studies using the same approach and clinical cohorts of periodontitis of various strengths also identified augmented levels of amino acids, alcohols, short chain fatty acids and sugars in the disease group [36][37][38][39]. Apart from 1 H-NMR, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) were also employed to detect metabolic signatures associated with the periodontitis.…”

Section: Gingivitis and Periodontal Diseasementioning

confidence: 99%

Unravelling the Potential of Salivary Volatile Metabolites in Oral Diseases. A Review

et al. 2020

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Fostered by the advances in the instrumental and analytical fields, in recent years the analysis of volatile organic compounds (VOCs) has emerged as a new frontier in medical diagnostics. VOCs analysis is a non-invasive, rapid and inexpensive strategy with promising potential in clinical diagnostic procedures. Since cellular metabolism is altered by diseases, the resulting metabolic effects on VOCs may serve as biomarkers for any given pathophysiologic condition. Human VOCs are released from biomatrices such as saliva, urine, skin emanations and exhaled breath and are derived from many metabolic pathways. In this review, the potential of VOCs present in saliva will be explored as a monitoring tool for several oral diseases, including gingivitis and periodontal disease, dental caries, and oral cancer. Moreover, the analytical state-of-the-art for salivary volatomics, e.g., the most common extraction techniques along with the current challenges and future perspectives will be addressed unequivocally.

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Metabolomic Status of The Oral Cavity in Chronic Periodontitis

Cited by 34 publications

References 59 publications

Understanding LrgAB Regulation of Streptococcus mutans Metabolism

Understanding LrgAB Regulation of Streptococcus mutans Metabolism

Acetate and Potassium Modulate the Stationary-Phase Activation of lrgAB in Streptococcus mutans

Unravelling the Potential of Salivary Volatile Metabolites in Oral Diseases. A Review

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