Metabolomic studies of anthocyanins in fruits by means of a liquid chromatography coupled to mass spectrometry workflow

Oliveira, Joana Paula da Silva; Oliveira, Renatha Tavares de; Guedes, Amanda Lima; Oliveira, Matheus da Costa; Macedo, Andrea Furtado

doi:10.1016/j.cpb.2022.100260

Cited by 10 publications

(1 citation statement)

References 186 publications

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“…For example, in plant metabolomics experiments, biological materials are often dried to stabilize them and prevent enzymatic activities and metabolite decomposition [ 14 , 15 , 16 ]. This technique can also be applied to, e.g., dry fruits for the study of anthocyanins, which are used in food and pharmaceutical industries as natural pigments [ 17 ]; dry vegetable samples prior to NMR spectroscopy analysis [ 15 ]; prepare urine samples for headspace-SPME-GC-MS analysis of volatile organic compounds [ 18 ]; dry fecal samples to preserve microbial and chemical stability, as well as standardize samples by dry weight for gut microbiota studies [ 19 ]; and to dry tissue powder for biological and clinical research [ 20 ]. Furthermore, lyophilization can be used to reduce sample volume and to concentrate compounds in aqueous samples that would otherwise be below the limit of detection [ 21 ], e.g., plant root exudates [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Untargeted Plant Metabolomics: Evaluation of Lyophilization as a Sample Preparation Technique

Maisl,

Doppler,

Seidl

et al. 2023

Metabolites

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Lyophilization is a common method used for stabilizing biological samples prior to storage or to concentrate extracts. However, it is possible that this process may alter the metabolic composition or lead to the loss of metabolites. In this study, the performance of lyophilization is investigated in the example of wheat roots. To this end, native and 13C-labelled, fresh or already lyophilized root samples, and (diluted) extracts with dilution factors up to 32 and authentic reference standards were investigated. All samples were analyzed using RP-LC-HRMS. Results show that using lyophilization for the stabilization of plant material altered the metabolic sample composition. Overall, 7% of all wheat metabolites detected in non-lyophilized samples were not detected in dried samples anymore, and up to 43% of the remaining metabolites exhibited significantly increased or decreased abundances. With respect to extract concentration, less than 5% of the expected metabolites were completely lost by lyophilization and the recovery rates of the remaining metabolites were slightly reduced with increasing concentration factors to an average of 85% at an enrichment factor of 32. Compound annotation did not indicate specific classes of wheat metabolites to be affected.

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Section: Introductionmentioning

confidence: 99%

Untargeted Plant Metabolomics: Evaluation of Lyophilization as a Sample Preparation Technique

Maisl,

Doppler,

Seidl

et al. 2023

Metabolites

View full text Add to dashboard Cite

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Understanding LC/MS‐Based Metabolomics: A Detailed Reference for Natural Product Analysis

Sarkar,

Singh,

Chandel

2024

Proteomics Clinical Apps

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Liquid chromatography, when used in conjunction with mass spectrometry (LC/MS), is a powerful tool for conducting accurate and reproducible investigations of numerous metabolites in natural products (NPs). LC/MS has gained prominence in metabolomic research due to its high throughput, the availability of multiple ionization techniques and its ability to provide comprehensive metabolite coverage. This unique method can significantly influence various scientific domains. This review offers a comprehensive overview of the current state of LC/MS‐based metabolomics in the investigation of NPs. This review provides a thorough overview of the state of the art in LC/MS‐based metabolomics for the investigation of NPs. It covers the principles of LC/MS, various aspects of LC/MS‐based metabolomics such as sample preparation, LC modes, method development, ionization techniques and data pre‐processing. Moreover, it presents the applications of LC/MS‐based metabolomics in numerous fields of NPs research such as including biomarker discovery, the agricultural research, food analysis, the study of marine NPs and microbiological research. Additionally, this review discusses the challenges and limitations of LC/MS‐based metabolomics, as well as emerging trends and developments in this field.

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Fruits waste in packaging applications

Gupta,

Rout,

Guha

et al. 2024

Adding Value to Fruit Wastes

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Metabolomic studies of anthocyanins in fruits by means of a liquid chromatography coupled to mass spectrometry workflow

Cited by 10 publications

References 186 publications

Untargeted Plant Metabolomics: Evaluation of Lyophilization as a Sample Preparation Technique

Untargeted Plant Metabolomics: Evaluation of Lyophilization as a Sample Preparation Technique

Understanding LC/MS‐Based Metabolomics: A Detailed Reference for Natural Product Analysis

Fruits waste in packaging applications

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