Metabolomic study of volatile compounds emitted by lavender grown under open-field conditions: a potential approach to investigate the yellow decline disease

Stierlin, Émilie; Nicolè, Florence; Costes, Thomas; Fernández, Xavier; Michel, Thomas

doi:10.1007/s11306-020-01654-6

Cited by 17 publications

(15 citation statements)

References 33 publications

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“…using conventional analyses were detected (Table 1). Differences, particularly in linalool/linalyl acetate ratios, were also observed between the profiles obtained using the NT device combined with the Torion T‐9 portable GC–MS device and those obtained via DHS followed by analysis using a bench‐top instrument 14 . These ratio changes may partly result from co‐elution between linalool and cis ‐limonene oxide in the portable GC–MS device.…”

Section: Resultsmentioning

confidence: 88%

“…Considering an extraction time of 5 min and an analysis time of 3 min, five extractions and analyses can be performed per hour in the field. However, only 14 of the 50 compounds identified by Stierlin et al 14 . using conventional analyses were detected (Table 1).…”

Section: Resultsmentioning

confidence: 97%

“…Linalool and linalyl acetate were identified as major compounds, but β‐phellandrene and 1,8‐cineole were not detected, contrary to our results on diva fine lavender. Recently, volatile compounds produced by fine lavender and lavandin were collected in‐field using the DHS approach 14 . Compounds trapped were further thermodesorbed and analysed in the laboratory with an ATD‐GC–MS device.…”

Section: Resultsmentioning

confidence: 99%

“…A previous study recently published by our group used a dynamic headspace (DHS) device to trap VOCs emitted by different varieties of lavender in the field. Volatile compounds were then analysed in the laboratory using an automated thermal desorption (ATD)‐GC–MS 14 . A total of 50 compounds were identified in lavender and lavandin.…”

Section: Introductionmentioning

confidence: 99%

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Field analyses of lavender volatile organic compounds: performance evaluation of a portable gas chromatography–mass spectrometry device

Stierlin

Michel

Fernández

2020

Phytochemical Analysis

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Introduction: In situ analysis of volatile organic compounds (VOCs) emitted by plants is an important challenge in chemical ecology. The traditional approach usually consists in trapping compounds using dynamic headspace extraction (DHS) in-field, followed by gas chromatography analysis coupled with mass spectrometry (GC-MS and/or GC-FID) in the laboratory. Objectives: In this study, we evaluated the use of the new portable Torion T-9 GC-MS system for rapid and in situ analysis of VOCs emitted by fine lavender and lavandin species. Material and methods: All field analyses were performed using a person-portable low-thermal mass GC system coupled with a miniature toroidal ion trap mass analyser (ppGC-ITMS): Torion T-9 portable GC-MS. Subsequently, multivariate statistical analyses were performed to determine chemical differences between species. Results: Thirty compounds were separated and detected in all lavender aboveground samples in only 3 min of analysis. Conclusions: The portable GC-MS device enabled a rapid in-field distinction of Lavandula species based on their detected volatile profiles. In this study, we evaluate the use of the new portable TORION T-9 GC-MS system for rapid and in situ analysis of volatile organic compounds (VOCs) emitted by fine lavender and lavandin species. Thirty compounds were separated and detected from all lavender aerial part samples in only three minutes of analysis. The ability to quickly characterize the VOCs emitted by plants in the field attests of this portable device's potential compared to long laboratory analyses.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Field analyses of lavender volatile organic compounds: performance evaluation of a portable gas chromatography–mass spectrometry device

Stierlin

Michel

Fernández

2020

Phytochemical Analysis

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“…We suggested that lavender and lavandin produce root exudates whose quality and quantity, specific to each species, could vary according to the plant health status thus impacting AM symbiosis. It is also possible that diseased lavender and lavandin could reduce the C supply necessary for AMF development due to the modification in carbohydrate synthesis and transport as shown in phytoplasma-infected plants 23,24 . Plant health status effect was evidenced in both types of agricultural practices, however the fact that it was significantly greater in lavender than in lavandin (30% vs 15%, Table S1) could suggest the influence of the host plant identity on root AMF colonization [25][26][27] .…”

Section: Discussionmentioning

confidence: 99%

Plant health status effects on arbuscular mycorrhizal fungi associated with Lavandula angustifolia and Lavandula intermedia infected by Phytoplasma in France

Binet

Marchal

Lipuma³

et al. 2020

Sci Rep

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We investigated root communities of arbuscular mycorrhizal fungi (AMF) in relation to lavender (Lavandula angustifolia) and lavandin (Lavandula intermedia) health status from organic and conventional fields affected by Phytoplasma infection. The intensity of root mycorrhizal colonization was significantly different between diseased and healthy plants and was higher in the latter regardless of agricultural practice. This difference was more pronounced in lavender. The root AMF diversity was influenced by the plant health status solely in lavender and only under the conventional practice resulting in an increase in the AMF abundance and richness. The plant health status did not influence the distribution of root AMF communities in lavandin unlike its strong impact in lavender in both agricultural practices. Finally, among the most abundant molecular operational taxonomic units (MOTUs), four different MOTUs for each plant species were significantly abundant in the roots of healthy lavender and lavandin in either agricultural practice. Our study demonstrated that the plant health status influences root colonization and can influence the diversity and distribution of root AMF communities. Its effects vary according to plant species, can be modified by agricultural practices and allow plants to establish symbiosis with specific AMF species.

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A comparison between mobile and stationary gas chromatography–mass spectrometry devices for analysis of complex volatile profiles

et al. 2022

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On-site analysis of volatile organic compounds (VOCs) with miniaturized gas chromatography–mass spectrometry (GC–MS) systems is a very rapidly developing field of application. While, on the one hand, major technological advances are improving the availability of these systems on the market, on the other hand, systematic studies to assess the performance of such instruments are still lacking. To fill this gap, we compared three portable GC–MS devices to a state-of-the-art benchtop (stationary) system for analysis of a standard mixture of 18 VOCs. We systematically compared analytical parameters such as the sensitivity and similarity of the signal response pattern and the quality of the obtained mass spectra. We found that the investigated mobile instruments (i) showed different response profiles with a generally lower number of identified analytes. Also, (ii) mass spectral reproducibility (% relative standard deviation (RSD) of the relative abundance of selective fragments) was generally worse in the mobile devices (mean RSD for all targeted fragments ~9.7% vs. ~3.5% in the stationary system). Furthermore, mobile devices (iii) showed a poorer mass spectral similarity to commercial reference library spectra (>20% deviation of fragment ion relative intensity vs. ~10% in the stationary GC–MS), suggesting a less reliable identification of analytes by library search. Indeed, (iv) the performance was better with higher-mass and/or more abundant fragments, which should be considered to improve the results of library searches for substance identification. Finally, (v) the estimation of the signal-to-noise ratio (S/N) in mobile instruments as a measure of sensitivity revealed a significantly lower performance compared to the benchtop lab equipment (with a ratio among medians of ~8 times lower). Overall, our study reveals not only a poor signal-to-noise ratio and poor reproducibility of the data obtained from mobile instruments, but also unfavorable results with respect to a reliable identification of substances when they are applied for complex mixtures of volatiles. Graphical Abstract

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Metabolomic study of volatile compounds emitted by lavender grown under open-field conditions: a potential approach to investigate the yellow decline disease

Cited by 17 publications

References 33 publications

Field analyses of lavender volatile organic compounds: performance evaluation of a portable gas chromatography–mass spectrometry device

Field analyses of lavender volatile organic compounds: performance evaluation of a portable gas chromatography–mass spectrometry device

Plant health status effects on arbuscular mycorrhizal fungi associated with Lavandula angustifolia and Lavandula intermedia infected by Phytoplasma in France

A comparison between mobile and stationary gas chromatography–mass spectrometry devices for analysis of complex volatile profiles

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