Metabolomics Analysis of C. lanceolatum Roots in the Presence of Its Endophytic Fungi

Abstract

The aim of this work was to identify and quantify the metabolites present in the roots of Combretum lanceolatum inoculated with its endophytic fungi. The metabolomics was accomplished using the 1H nuclear magnetic resonance (NMR) spectral data and evaluated via rNMR software and Madison Metabolomics Consortium Database (MMCD). The principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) showed that plants inoculated with Trichoderma spirale (Ts) present differentiation and discrimination over the time as compared to control. Seven days after Ts fungal inoculation, 15 metabolites were identified at different concentrations comparing to the control plants. The plants inoculated with Ts fungus present the metabolites spermidine and pantothenate in higher concentrations and 3-hydroxybutyric acid and β-alanine in lower concentrations as compared to control plants, indicating any response to biotic stress. These metabolites are involved in various plant processes, including secondary metabolites biosynthesis, energy metabolism and self-defense. Therefore, this work demonstrates the diversification of primary metabolites composition influenced by endophytes inoculation.

Discovery of Potential Anti-Microbial Molecules and Spectrum Correlation Effect of Ardisia crenata Sims via High-Performance Liquid Chromatography Fingerprints and Molecular Docking

“…Modern analytical techniques have played an important role in the quality identification of TCM, including the detection of hydrazine in real water and soil samples from the growing areas of TCM [ 25 ]. In particular, integrated metagenomics and meta-transcriptomics sequencing can also be used to examine the abundance of microbial consortiums and their metabolites [ 26 , 27 , 28 ]. Macromolecular phase separation was also used to deliver bioactive compounds [ 29 ].…”

Potencial inseticida de >i<Trichoderma>/i< spp. produzidos por fermentação líquida

“…A mortalidade de adultos do percevejo-marrom variou entre os isolados (F = 2. LUCON et al, 2009;SERRANO et al, 2023;SUMIDA et al, 2018;RIBEIRO et al, 2023). Ainda neste contexto, dois isolados foram identificados como T. endophyticum, sendo que essa espécie possui um produto registrado no MAPA e dispõe de diversos estudos no Brasil (BARROS et al, 2019;dos SANTOS et al, 2022).…”