Metabolomics analysis reveals global acetoin stress response of Bacillus licheniformis

Yuan, Honglun; Xu, Yong; Chen, Yaozhong; Zhan, Yangyang; Wei, Xuetuan; Li, Lü; Wang, Dong; He, Penghui; Li, Shengqing; Chen, Shouwen

doi:10.1007/s11306-019-1492-7

Cited by 48 publications

(30 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The occurrence of positive correlations between metal induced oxidative stress and high levels of bacteria-born phytohormones, ability to produce siderophores and other PGP traits of bacteria, and the high plant dry mass and high primary root length, is notable [ 72 , 73 ]. It was also earlier documented that, in the presence of metals, acetoin produced by bacteria improves the oxidative stress resistance, the ACC-deaminase activity is higher [ 74 , 75 , 76 ], IAA synthesis is higher and this can promote the formation of lateral and adventitious roots, and as a result can enhance mineral uptake by plants [ 18 , 68 , 69 , 77 ].…”

Section: Resultsmentioning

confidence: 99%

Trifolium repens-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps

Oleńska

Imperato

Małek

et al. 2020

Plants

View full text Add to dashboard Cite

Heavy metals in soil, as selective agents, can change the structure of plant-associated bacterial communities and their metabolic properties, leading to the selection of the most-adapted strains, which might be useful in phytoremediation. Trifolium repens, a heavy metal excluder, naturally occurs on metal mine waste heaps in southern Poland characterized by high total metal concentrations. The purpose of the present study was to assess the effects of toxic metals on the diversity and metabolic properties of the microbial communities in rhizospheric soil and vegetative tissues of T. repens growing on three 70–100-years old Zn–Pb mine waste heaps in comparison to Trifolium-associated bacteria from a non-polluted reference site. In total, 113 cultivable strains were isolated and used for 16S rRNA gene Sanger sequencing in order to determine their genetic affiliation and for in vitro testing of their plant growth promotion traits. Taxa richness and phenotypic diversity in communities of metalliferous origin were significantly lower (p < 0.0001) compared to those from the reference site. Two strains, Bacillus megaterium BolR EW3_A03 and Stenotrophomonas maltophilia BolN EW3_B03, isolated from a Zn–Pb mine waste heap which tested positive for all examined plant growth promoting traits and which showed co-tolerance to Zn, Cu, Cd, and Pb can be considered as potential facilitators of phytostabilization.

show abstract

Section: Resultsmentioning

confidence: 99%

Trifolium repens-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps

Oleńska

Imperato

Małek

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…Therefore, the toxicity of the very high acetoin concentrations will no doubt prevent glucose assimilation and acetoin production of CGS11, and even activate its underlying acetoin catabolism. Since acetoin metabolism is complex and still not clearly elucidated, several successful strategies, such as physical/ chemical mutagenesis [54], adaptive evolution [52,53] or omics focusing on global transcriptional/metabolism level responses to acetoin stress [55], could be adopted to deeply understand and improve the acetoin tolerance of C. glutamicum in future studies.…”

Section: Fed-batch Fermentation Of Cgs11 For Efficient (3r)-acetoin Pmentioning

confidence: 99%

Engineering central pathways for industrial-level (3R)-acetoin biosynthesis in Corynebacterium glutamicum

Mao

Kou

et al. 2020

Microb Cell Fact

View full text Add to dashboard Cite

Background: Acetoin, especially the optically pure (3S)-or (3R)-enantiomer, is a high-value-added bio-based platform chemical and important potential pharmaceutical intermediate. Over the past decades, intense efforts have been devoted to the production of acetoin through green biotechniques. However, efficient and economical methods for the production of optically pure acetoin enantiomers are rarely reported. Previously, we systematically engineered the GRAS microorganism Corynebacterium glutamicum to efficiently produce (3R)-acetoin from glucose. Nevertheless, its yield and average productivity were still unsatisfactory for industrial bioprocesses. Results: In this study, cellular carbon fluxes in the acetoin producer CGR6 were further redirected toward acetoin synthesis using several metabolic engineering strategies, including blocking anaplerotic pathways, attenuating key genes of the TCA cycle and integrating additional copies of the alsSD operon into the genome. Among them, the combination of attenuation of citrate synthase and inactivation of phosphoenolpyruvate carboxylase showed a significant synergistic effect on acetoin production. Finally, the optimal engineered strain CGS11 produced a titer of 102.45 g/L acetoin with a yield of 0.419 g/g glucose at a rate of 1.86 g/L/h in a 5 L fermenter. The optical purity of the resulting (3R)-acetoin surpassed 95%. Conclusion: To the best of our knowledge, this is the highest titer of highly enantiomerically enriched (3R)-acetoin, together with a competitive product yield and productivity, achieved in a simple, green processes without expensive additives or substrates. This process therefore opens the possibility to achieve easy, efficient, economical and environmentally-friendly production of (3R)-acetoin via microbial fermentation in the near future.

show abstract

“…Toxicity of acetoin can hinder its higher production by various microorganisms. It was reported that an approximate 30% cell growth of B. licheniformis will be inhibited with 40 g/L exogenous acetoin (Yuan et al, 2019). Thus, production of 2,3-butanediol may be due to induction of an unidentified acetoin reductase or meso-2,3-butanediol dehydrogenase to resistant the toxicity of acetoin at high concentrations.…”

Section: Fed-batch Fermentation Of Acetoin By B Licheniformis Mw3 ( mentioning

confidence: 99%

Metabolic Engineering of Bacillus licheniformis for Production of Acetoin

Lü

Cao

Guo

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Acetoin is a potential platform compound for a variety of chemicals. Bacillus licheniformis MW3, a thermophilic and generally regarded as safe (GRAS) microorganism, can produce 2,3-butanediol with a high concentration, yield, and productivity. In this study, B. licheniformis MW3 was metabolic engineered for acetoin production. After deleting two 2,3-butanediol dehydrogenases encoding genes budC and gdh, an engineered strain B. licheniformis MW3 (budC gdh) was constructed. Using fedbatch fermentation of B. licheniformis MW3 (budC gdh), 64.2 g/L acetoin was produced at a productivity of 2.378 g/[L h] and a yield of 0.412 g/g from 156 g/L glucose in 27 h. The fermentation process exhibited rather high productivity and yield of acetoin, indicating that B. licheniformis MW3 (budC gdh) might be a promising acetoin producer.

show abstract

Metabolomics analysis reveals global acetoin stress response of Bacillus licheniformis

Cited by 48 publications

References 37 publications

Trifolium repens-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps

Trifolium repens-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps

Engineering central pathways for industrial-level (3R)-acetoin biosynthesis in Corynebacterium glutamicum

Metabolic Engineering of Bacillus licheniformis for Production of Acetoin

Contact Info

Product

Resources

About