Metabolomics and Dereplication Study of the Endophytic Fungus Aspergillus chevelieri in Search of Bioactive Natural Compounds

Abstract

Objectives: This study aimed to point the significant rule of metabolomics tools to assess the chemistry of the bioactive metabolites produced by endophytic fungus Aspergillus chevalieri isolated from Lagerostromia tomentosa C. presl. The anticancer of crude extracts, fractions and pure compounds and antimicrobial of pure compounds were investigated as part of this study. Methods: An endophytic fungus (Aspergillus chevalieri) was isolated from the tissues of the stem of Lagerostromia tomentosa C. Presl and identified through molecular biological procedure by DNA isolation, PCR, DNA sequencing and through searching the Gene Bank. Metabolomics profiling and dereplication studies were employed to choose the optimum growth medium and conditions that yield the most significant metabolites. The crude extract of the 30-days rice culture of Aspergillus chevalieri was subjected to bioactivity and metabolomics guided isolation approach. The structure of the isolated compounds was determined on the basis of 1D, 2D NMR and mass spectrometry (HR-ESIMS) analysis. Results: four fractions were further purified to produce five pure compounds, which are Ergosterol (1), Ergosterol peroxide (2), Campesterol (3), Flavoglaucin (4) and 3-O-methyl caffeic acid (5). Multivariate data analysis highlighted the most significant metabolites contributed to the bioactivity. The pure compounds were tested for the anticancer and antimicrobial activity, compound (1) exhibited significant antitrypanosomal activity, while compounds (2, 3, 4 and 5) effectively inhibited the growth of Escherichia coli, Staphylococcus aureus and Candida albicans. Conclusion: A combination of metabolomic-and bioassay-guided approaches gives an access to a shorter and faster route to highlight the active metabolites, which are highly correlated to the bioactivity during the first stage of fractionation.

The synergistic effect of biosynthesized silver nanoparticles from a combined extract of parsley, corn silk, and gum arabic: in vivo antioxidant, anti-inflammatory and antimicrobial activities

“…Metabolomic profiling of P. crispum, S. maydis and a combination of the three crude extracts Metabolomic profiling was performed on the crude extracts of P. crispum, S. maydis and a combination of the three crude extracts to deliver general qualitative and quantitative profiles of metabolites that may be involved in the activity of the extracts [38]. Dereplication refers to the rapid identification of the known secondary metabolites and their quantification in crude unfractionated extracts [39,40]. Identification of metabolome was done on an Acquity Liquid Chromatography (LC) system coupled to a Synapt G2 HDMS quadrupole time-offlight hybrid mass spectrometer (Waters, Milford, USA).…”

Increment of Lysosomal Biogenesis by Combined Extracts of Gum Arabic, Parsley, and Corn Silk: A Reparative Mechanism in Mice Renal Cells

“…Metabolomic profiling was performed on crude extracts of P. crispum and S. maydis and a combination of the three extracts to deliver general qualitative and quantitative profiles of metabolites that may be involved in the activity of the extracts [14,15]. Dereplication refers to the rapid identification of known secondary metabolites and their quantification in crude unfractionated extracts [16,17]. LC-MS measurement was done on an Acquity Liquid Chromatography (LC) system coupled to a Synapt G2 HDMS quadrupole time-of-flight hybrid mass spectrometer (Waters, Milford, USA).…”

Comparison of Aspergillus chevalieri and related species in dark tea at different aspects: Morphology, enzyme activity and mitochondrial genome

“…Additionally, researchers believed that A. chevalieri had a positive effect on the quality of dark tea (Chen, Hubka, et al., 2017; Huang et al., 2017). A. chevalieri metabolites have a variety of activities, such as the inhibition of bacteria and plant pathogens’ growth (Bovio et al., 2019; Zin et al., 2017), protection of human fibroblasts under oxidative stress (Letsiou et al., 2020), inhibition of hepatitis C virus (Hawas et al., 2015), anti‐tumor effect (Tawfik et al., 2017), and fermentation of mycophenolic acid (Mouhamadou et al., 2017). It is a resource strain that could use more food technology (Liu, Zhou, Wang, Tan, et al., 2021; Liu, Zhou, Wang, Ye, et al., 2021) to develop active ingredients.…”

“…, anti-tumor effect (Tawfik et al, 2017), and fermentation of mycophenolic acid (Mouhamadou et al, 2017). It is a resource strain that could use more food technology (Liu, Zhou, Wang, Tan, et al, 2021;Liu, Zhou, Wang, Ye, et al, 2021) to develop active ingredients.…”