Metabolomics approach of<i>Symphyotrichum squamatum</i>ethanol extract and its nano-Ag formulation protective effect on gastric ulcer<i>via</i>bio-chemical and pathological analyses

Hassan, Heba A.; Ayoub, Iriny M.; Ragab, Tamer I.M.; Afifi, Sherif M.; Gendy, Abd El‐Nasser G. El; Farrag, Abdel Razik H.; Abd‐ElGawad, Ahmed M.; Farag, Mohamed A.; Elshamy, Abdelsamed I.; Ammara, Naglaa M

doi:10.1080/1354750x.2022.2157488

Cited by 8 publications

(11 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After extraction, the UPLC–ESI-MS profiling of the plant extract was performed using an Acquity UPLC system (Waters, Germany) equipped with an HSS T3 column (100 × 1.0 mm, particle size 1.8 µm; Waters), applying the same parameters reported by Ayoub et al ( 2022 ), Hassan et al ( 2023 ), and Abib et al ( 2023 ).…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS

Ibrahim,

Sayed,

Abdelhameed

et al. 2024

Inflammopharmacol

Self Cite

View full text Add to dashboard Cite

Erigeron bonariensis is widely distributed throughout the world's tropics and subtropics. In folk medicine, E. bonariensis has historically been used to treat head and brain diseases. Alzheimer’s disease (AD) is the most widespread form of dementia initiated via disturbances in brain function. Herein, the neuroprotective effect of the chemically characterized E. bonariensis ethanolic extract is reported for the first time in an AD animal model. Chemical profiling was conducted using UPLC–ESI-MS analysis. Female rats underwent ovariectomy (OVX) followed by 42 days of D-galactose (D-Gal) administration (150 mg/kg/day, i.p) to induce AD. The OVX/D-Gal-subjected rats received either donepezil (5 mg/kg/day) or E. bonariensis at 50, 100, and 200 mg/kg/day, given 1 h prior to D-Gal. UPLC–ESI-MS analysis identified 42 chemicals, including flavonoids, phenolic acids, terpenes, and nitrogenous constituents. Several metabolites, such as isoschaftoside, casticin, velutin, pantothenic acid, xanthurenic acid, C18-sphingosine, linoleamide, and erucamide, were reported herein for the first time in Erigeron genus. Treatment with E. bonariensis extract mitigated the cognitive decline in the Morris Water Maze test and the histopathological alterations in cortical and hippocampal tissues of OVX/D-Gal-subjected rats. Moreover, E. bonariensis extract mitigated OVX/D-Gal-induced Aβ aggregation, Tau hyperphosphorylation, AChE activity, neuroinflammation (NF-κBp65, TNF-α, IL-1β), and apoptosis (Cytc, BAX). Additionally, E. bonariensis extract ameliorated AD by increasing α7-nAChRs expression, down-regulating GSK-3β and FOXO3a expression, and modulating Jak2/STAT3/NF-ĸB p65 and PI3K/AKT signaling cascades. These findings demonstrate the neuroprotective and memory-enhancing effects of E. bonariensis extract in the OVX/D-Gal rat model, highlighting its potential as a promising candidate for AD management. Graphical Abstract

show abstract

Section: Methodsmentioning

confidence: 99%

Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS

Ibrahim,

Sayed,

Abdelhameed

et al. 2024

Inflammopharmacol

Self Cite

View full text Add to dashboard Cite

show abstract

“…For the profiling of LME using UPLC-TOF-MS, 1 g was extracted using 70% EtOH in an ultrasonic bath (Branson ultrasonic corporation, Danbury, CT, USA) for 1 h. After filtering and centrifugation for 15 min at 12,000× g , the clear supernatant was used for UPLC-qTOF-MS analysis. The UPLC-qTOF-MS analysis of the extract was conducted under the exact conditions reported in [ 10 , 18 ]. The used HSS T3 column (100 × 1.0 mm, 1.8 m particle size; Waters) was installed on an ACQUITY UPLC system (Waters, Milford, MA, USA) equipped with a 6540 Ultra-High-Definition (UHD) Accurate-Mass Q-TOFLC/MS (Agilent, Palo Alto, CA, USA), coupled to an ESI interface, and was operated in a positive or negative ion mode.…”

Section: Methodsmentioning

confidence: 99%

“…A Shimadzu UV-2401 (PC) S, UV-Vis spectrophotometer was used and operated using Spectrum TM Version 6.87 (Shimadzu, Japan) scanning from 200–800 nm. The maximum absorption wavelength, λ max was then noted [ 18 ]. To assess the particle size and shape, a JEOL JEM 1011 (JEOL Ltd., Tokyo, Japan) transmission electron microscope was employed.…”

Section: Methodsmentioning

confidence: 99%

“…The creation of nano-formulations of plant extracts has an enormous potential in the field of nanomedicine, including boosting their biological effects through augmentation of the active biomolecule’s concentration [ 16 ]. Due to their distinctive applications in pharmaceutics, agriculture, water detoxification, air filtration, textile industries, and as a catalyst, silver nanoparticles have received increasing attention among these nano-formulations [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protective Action Mechanisms of Launaea mucronata Extract and Its Nano-Formulation against Nephrotoxicity in Rats as Revealed via Biochemical, Histopathological, and UPLC-QTOF–MS/MS Analyses

et al. 2023

Self Cite

View full text Add to dashboard Cite

Plants belonging to the Launaea genus have been extensively utilized ethnopharmacologically to treat a variety of diseases, including kidney disorders. Chromium is a common industrial pollutant that has been linked to kidney disease. The present work was designed for the investigation of the UPLC-QTOF–MS/MS metabolite profile of the L. mucronate ethanolic extract (LME), along with assessing the mechanistic protective actions of LME and its nano-silver formulation (LMNS) against K2Cr2O7-induced nephrotoxicity in rats. LMNE was successfully biosynthesized and confirmed using UV–Visible (UV–Vis) spectroscopy and transmission electron microscopy (TEM). The nephroprotective effects of LME and LMNE was assessed in rats exposed to potassium dichromate (K2Cr2O7, 15 mg/kg BW) to cause nephrotoxicity. LME and LMNS, separately, were administered twice daily for 14 days at doses of 200 and 400 mg/kg BW, respectively. The kidney function, catalase, UGT, Nrf2, PGE2, Cox-2, ERK, and MAPK levels in renal tissue were all assessed, along with histopathological examinations for exploring their ameliorative effects. Forty-five bioactive metabolites were annotated belonging to flavonoids, phenolic and organic acids, coumarins, and fatty acids. Metabolite profiling revealed that chlorogenic acid, apigenin, and luteolin glycosides were the main phenolics, with chlorogenic acid-O-hexoside reported for the first time in LME. The findings revealed that the serum kidney function indicators (urea and creatinine) were markedly elevated in K2Cr2O7-intoxicated rats. Furthermore, inflammatory indicators (COX-2 and PGE2), MAPK, and ERK were all markedly elevated in kidney tissue, whereas catalase, UGT, and Nrf2 levels were downregulated. Histological and immunohistochemical assays confirmed the toxic effects of K2Cr2O7 in the kidneys. In contrast, the administration of LME and LMNS prior to K2Cr2O7 considerably improved the architecture of the renal tissue, while also restoring levels of most biochemical markers. Functioning via the inhibition of the MAPK/ERK pathway, activating Nrf2, and modifying the antioxidant and metabolic enzymes, LME and LMNS exerted their nephroprotective effects against K2Cr2O7-induced toxicity.

show abstract

“…Secondary metabolites produced by plants have been known for their significant therapeutic effects. [49][50][51][52][53] Several medicinal plants have been reported to treat cancer [54][55][56][57] or aid in decreasing the side effects of chemotherapeutic drugs. [58] Several species in family Proteaceae have been reported to exert potent hepatoprotective, antioxidant, and anti-inflammatory activities.…”

Section: Effect Of S Sinuatus Methanol Leaf Extract Treatment On Infl...mentioning

confidence: 99%

In vivo hepatoprotective and nephroprotective effects ofStenocarpus sinuatusleaf extract against ifosfamide‐induced toxicity in rats

Younis,

Ayoub,

George

et al. 2023

Archiv der Pharmazie

Self Cite

View full text Add to dashboard Cite

Ifosfamide (IFOS) is a broad‐spectrum chemotherapeutic agent that has been extensively used for breast cancer and other solid tumors. Unfortunately, its use is associated with toxicities of several organs. Stenocarpus sinuatus is an Australian tree belonging to the Proteaceae family. In the current study, the phytochemical constituents of S. sinuatus methanol leaf extract (SSLE) were assessed. In addition, the protective effect of SSLE against IFOS‐induced nephrotoxicity and hepatotoxicity was evaluated. Rats were randomly divided into six groups: control, IFOS (50 mg/kg), IFOS + SSLE (100 mg/kg), IFOS + SSLE (200 mg/kg), IFOS + SSLE (400 mg/kg), and SSLE (400 mg/kg). Hepatoprotective and nephroprotective potency of SSLE was assessed using different biochemical parameters. The phytochemical investigation resulted in the isolation of four flavonoid glycosides (kaempferol 3‐O‐β‐ d‐glucopyranosyl‐(1→2)‐α‐ l‐rhamnopyranoside, kaempferol 3‐O‐α‐rhamnopyranoside, isorhamnetin 3‐O‐β‐ d‐glucopyranosyl‐(1→2)‐α‐ l‐rhamnopyranoside, and quercetin 3‐O‐β‐ d‐glucopyranosyl‐(1→2)‐α‐ l‐rhamnopyranoside) and a coumarin (scopoletin). This is the first report on the isolated compounds from the genus Stenocarpus. SSLE showed enhancement of kidney and liver functions and reduction of oxidative stress and inflammation. The histopathology of the investigated organs confirmed the protective effect of SSLE. In conclusion, SSLE is considered as a promising candidate that can be used in defense against the toxic effects of IFOS after further clinical trials.

show abstract

Metabolomics approach ofSymphyotrichum squamatumethanol extract and its nano-Ag formulation protective effect on gastric ulcerviabio-chemical and pathological analyses

Cited by 8 publications

References 92 publications

Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS

Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS

Protective Action Mechanisms of Launaea mucronata Extract and Its Nano-Formulation against Nephrotoxicity in Rats as Revealed via Biochemical, Histopathological, and UPLC-QTOF–MS/MS Analyses

In vivo hepatoprotective and nephroprotective effects ofStenocarpus sinuatusleaf extract against ifosfamide‐induced toxicity in rats

Contact Info

Product

Resources

About