Metabolomics Based UHPLC-QToF-MS Approach for the Authentication of Various Botanicals and Dietary Supplements

Avula, Bharathi; Wang, YH; Isaac, Giorgis; Yuk, J; Wrona, Mark; Yu, Kwang Wan; Khan, IA

doi:10.1055/s-0036-1578583

Cited by 4 publications

(2 citation statements)

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“…Metabolomics has been employed to characterize the relationships between the metabolome of a given natural product and corresponding genotype, origin, quality, or other biotic or abiotic attributes. 28,[69][70][71] Two main analytical techniques are currently employed for metabolomic studies -MS and NMR. The relative advantages of these approaches have been discussed elsewhere.…”

Section: Authentication By Untargeted Metabolomicsmentioning

confidence: 99%

Selection and characterization of botanical natural products for research studies: a NaPDI center recommended approach

et al. 2019

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Section: Authentication By Untargeted Metabolomicsmentioning

confidence: 99%

Selection and characterization of botanical natural products for research studies: a NaPDI center recommended approach

et al. 2019

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“…Candidate structures were obtained and interpreted with available databases, including the Human Metabolome Database 29 , METLIN 30 , LIPID MAPS 31 . Additionally, acquired raw data were analyzed using Progenesis QI 32 , a small molecule discovery analysis software for LC-MS data.…”

Section: Methodsmentioning

confidence: 99%

In Utero Exposure to Histological Chorioamnionitis Primes the Exometabolomic Profiles of Preterm CD4+ T Lymphocytes

Matta

Sherrod

Marasco

et al. 2017

The Journal of Immunology

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Histological chorioamnionitis (HCA) is an intrauterine inflammatory condition increasing the risk for preterm birth, death, and disability due to persistent systemic and localized inflammation. The immunologic mechanisms sustaining this response in the preterm newborn remain unclear. We sought to determine the consequences of HCA exposure on the fetal CD4+ T lymphocyte exo-metabolome. We cultured naïve CD4+ T lymphocytes from HCA-positive and HCA-negative preterm infants matched for gestational age, sex, race, prenatal steroid exposure, and delivery mode. We collected conditioned media samples before and after a 6 hour in vitro activation of naïve CD4+ T lymphocytes with soluble Staphylococcal enterotoxin B (SEB) and anti-CD28. We analyzed samples by ultra performance liquid chromatography ion mobility-mass spectrometry (UPLC-IM-MS). We determined the impact of HCA on the CD4+ T lymphocyte exo-metabolome and identified potential biomarker metabolites by multivariate statistical analyses. We discovered that: (1) CD4+ T lymphocytes exposed to HCA exhibit divergent exo-metabolomic profiles in both naïve and activated states; (2) ~30% of detected metabolites differentially expressed in response to activation were unique to HCA-positive CD4+ T lymphocytes; and (3) metabolic pathways associated with glutathione detoxification and tryptophan degradation were altered in HCA-positive CD4+ T lymphocytes; and (4) flow cytometry and cytokine analyses suggested a bias towards a TH1-biased immune response in HCA-positive samples. HCA exposure primes the neonatal adaptive immune processes by inducing changes to the exo-metabolomic profiles of fetal CD4+ T lymphocytes. These exo-metabolomic changes may link HCA exposure to TH1 polarization of the neonatal adaptive immune response.

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Quality Consistency of Herbal Products: Chemical Evaluation

Osman

Chittiboyina

Avula

et al. 2023

Progress in the Chemistry of Organic Natural Products

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Metabolomics Based UHPLC-QToF-MS Approach for the Authentication of Various Botanicals and Dietary Supplements

Cited by 4 publications

References 0 publications

Selection and characterization of botanical natural products for research studies: a NaPDI center recommended approach

Selection and characterization of botanical natural products for research studies: a NaPDI center recommended approach

In Utero Exposure to Histological Chorioamnionitis Primes the Exometabolomic Profiles of Preterm CD4+ T Lymphocytes

Quality Consistency of Herbal Products: Chemical Evaluation

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