Metabolomics-centered mining of plant metabolic diversity and function: Past decade and future perspectives

Shen, Shuangqian; Zhan, Chuansong; Yang, Chenkun; Fernie, Alisdair R.; Luo, Jie

doi:10.1016/j.molp.2022.09.007

Cited by 110 publications

(53 citation statements)

References 220 publications

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“…Establishing the molecular mechanisms underlying 5M2NP’s toxicity and function as a defense regulator is an exciting prospect of this work. More generally, knowledge of the full functional repertoire of plant secondary metabolites will help to understand their ecology and evolution and lead to better applications for sustainable agriculture (Shen et al, 2023; Weng et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

The benzoxazinoid breakdown product 5-Methoxy-2-Nitrophenol has a dual function in maize defense against herbivory

Schimmel,

Escobar-Bravo,

Mateo

et al. 2023

Preprint

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Catabolism of plant secondary metabolites can yield bioactive breakdown products. However, these compounds often remain poorly characterized. Here, we report on the discovery, biosynthesis, and biological relevance of 5 Methoxy-2-Nitrophenol (5M2NP), a secondary metabolite breakdown product which accumulates in damaged maize tissues. We used mutant plants, biochemical complementation, and metabolomic analyses to determine the biosynthetic origin of 5M2NP. Additionally, we assessed 5M2NP′s role in plant defense against herbivores. For this, we surveyed defense-associated responses (gene expression, phytohormones, volatile organic compounds) in wounded, 5M2NP-supplemented maize leaves, and performed bioassays with herbivorous insects. We found that 5M2NP accumulation upon tissue disruption is contingent upon a functional benzoxazinoid biosynthesis pathway. Labeling experiments demonstrated that 5M2NP is derived from DIMBOA. Physiological doses of exogenous 5M2NP increased the wound-induced expression of defense genes and emission of terpenoids. Additionally, 5M2NP exhibited antibiotic and antixenotic activities towards both generalist and specialist herbivores in nano- to micromolar quantities. We conclude that 5M2NP, which represents a novel class of plant-derived compounds, can act as a direct defense and a defense modulator. 5M2NP thus expands the functional repertoire of benzoxazinoids and likely contributes to their protective function against insect herbivores. The discovery of 5M2NP highlights the multifunctionality of plant secondary metabolites and their breakdown products.

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Section: Discussionmentioning

confidence: 99%

The benzoxazinoid breakdown product 5-Methoxy-2-Nitrophenol has a dual function in maize defense against herbivory

Schimmel,

Escobar-Bravo,

Mateo

et al. 2023

Preprint

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“…The important role of metabolites and the factors governing metabolic variations among plants provides an insight to understand plant physiology and biology for better crop improvement in terms of yield and quality (Hong et al, 2016; Nakabayashi & Saito, 2015; Shen et al, 2023). Proteomics and metabolomics are both based on three technologies: fractionation to simplify complex mixtures; mass spectrometry (MS) of individual peptides and metabolites for their identification and quantification and bioinformatics analyses of observed data to corelate with genomic or metabolite databases (Singh et al, 2016).…”

Section: Omics Perspective Of Potassium Use Efficiency In Plantsmentioning

confidence: 99%

Potassium transport and use efficiency for sustainable fertigation in protected cropping

Bhardwaj

Huda

Jayasena

et al. 2023

J of Sust Agri & Env

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The increasing demand for high‐quality horticultural produces in global markets has driven the growing crop production under protected cropping, which are usually more efficient in fertilizer use compared to field cultivation. As one of the key macronutrients, available potassium (K+) resources have decreased due to the expansion of intensive agriculture and excessive use of K fertilizers. Currently, limited strategies have been adopted to improve crop quality in protected cropping with sustainable use of K+ fertigation and its comprehensive understanding at physiological and molecular levels. Therefore, we highlight the importance of optimal use of K+ in fertigation in protected cultivation that may also enhance crop quality characteristics. We review different K+ channels and transporters from various protein families responsible for K+ absorption and distribution across different plant tissues. An analysis of the literature on transcriptome, ionome, proteome and metabolome profiles of crops suggests the crucial roles of K+ in maintaining ion homoeostasis and modulating stress responses. It reveals that optimal K+ fertigation levels in protected cropping not only aids in maintaining the overall crop growth and production but also participates in maintaining the fruit quality. This review can potentially guide crop production and resource use efficiency in protected cropping, contributing to global food security and a better sustainable agricultural and environmental future.

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“…Thousands of metabolites can be detected in plant tissues using mass spectrometry. Metabolomics is a research discipline that integrates the capabilities of several types of research including analytical chemistry, statistics and biochemistry [ 8 ]. Metabolomics analysis involves the detection and quantification of metabolites with mass spectrometry or nuclear magnetic resonance, and integrates the resulting data with multivariate statistical techniques [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Analysis of Different Tissues of Lonicera japonica Thunb. Based on Liquid Chromatography with Mass Spectrometry

Wang

et al. 2023

Metabolites

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Lonicera japonica Thunb. (LJT) has been widely used as medicines or food additives in Asian countries for thousands of years. The flower buds are often medicinally used, and the other tissues are ignored. However, flowers, leaves and stems have also been reported to have antimicrobial, anti-inflammatory and antioxidant effects. In the current study, un-targeted metabolomics analysis was performed to investigate the metabolic difference among different tissues (flowers, flower buds, stems and leaves) of LJT based on liquid chromatography with mass spectrometry. A total of 171 metabolites were identified, including 28 flavonoids, 35 phenolic acids, 43 iridoids, 9 amino acids, 6 nucleotides, 16 fatty acids, 22 lipids and 12 others. Four new secondary metabolites were discovered. Some flavonoids and iridoids were not detected in leaves and stems. Principal component analysis showed significant differences among four different tissues. Some 27, 81, 113 differential metabolites were found between flowers/flower buds, leaves/flower buds, stems/flower buds, respectively. Primary metabolites showed a higher content in the flowers and flower buds. For the flavonoids, flavones were mainly accumulated in the leaves, flavonols were mainly accumulated in the flower buds, and acylated flavonol glucosides were mainly accumulated in the flowers. Most phenolic acids showed a higher content in the flowers or flower buds, while phenolic acid-glucosides showed significantly higher content in the flower buds. The most abundant iridoids in the LJT also showed a higher content in the flowers and flower buds. These results can provide new insights into the understanding of the metabolites changes in different tissues, and lay a theoretical foundation for the comprehensive utilization of LJT.

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Metabolomics-centered mining of plant metabolic diversity and function: Past decade and future perspectives

Cited by 110 publications

References 220 publications

The benzoxazinoid breakdown product 5-Methoxy-2-Nitrophenol has a dual function in maize defense against herbivory

The benzoxazinoid breakdown product 5-Methoxy-2-Nitrophenol has a dual function in maize defense against herbivory

Potassium transport and use efficiency for sustainable fertigation in protected cropping

Metabolomics Analysis of Different Tissues of Lonicera japonica Thunb. Based on Liquid Chromatography with Mass Spectrometry

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