Metabolomics combined with serum pharmacochemistry discovering the potential effective compounds of Fangji Huangqi Tang against nephrotic syndrome

Guo, Songjia; Yang, Liu; Zhang, Lichao; Li, Aiping

doi:10.1016/j.jchromb.2022.123532

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…The supernatant liquid was then collected for analysis using a high-performance liquid chromatography-triple quadrupole-mass spectrometer (HPLC-QQQ/MS) with a TSQ Endura (Thermo Fisher Scientific, Inc., Waltham, MA, USA) [17,18,19]. The analysis was performed in positive and negative electrospray ionization modes.…”

Section: Serum Pharmacochemistry Analysismentioning

confidence: 99%

Hypolipidemic activity and mechanism of active components of Dracocephalum moldavica L. tea

Li,

Zhang

et al. 2024

Food Science and Human Wellness

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Section: Serum Pharmacochemistry Analysismentioning

confidence: 99%

Hypolipidemic activity and mechanism of active components of Dracocephalum moldavica L. tea

Li,

Zhang

et al. 2024

Food Science and Human Wellness

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“…This formula is known for its effectiveness in augmenting Qi, raising Yang, promoting urination, and reducing swelling . In clinical practice, FHD is often used to treat conditions such as rheumatism, joint pain, edema, beriberi, frequent urination, and eczema . As documented, FHD consists of four herbs, namely, Stephaniae tetrandrae Radix (STR for short, Fangji in Chinese), Astragali Radix (AR for short, Huangqi in Chinese), Atractylodis macrocephalae Rhizoma (AMR for short, Baizhu in Chinese), and Glycyrrhizae Radix (GR for short, Gancao in Chinese) .…”

Section: Introductionmentioning

confidence: 99%

“… 12 In clinical practice, FHD is often used to treat conditions such as rheumatism, joint pain, edema, beriberi, frequent urination, and eczema. 13 As documented, FHD consists of four herbs, namely, Stephaniae tetrandrae Radix (STR for short, Fangji in Chinese), Astragali Radix (AR for short, Huangqi in Chinese), Atractylodis macrocephalae Rhizoma (AMR for short, Baizhu in Chinese), and Glycyrrhizae Radix (GR for short, Gancao in Chinese). 14 The main components of FHD include alkaloids (e.g., tetrandrine and fangchinoline) and flavonoids (e.g., astragaloside and glycyrrhizic acid).…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacology-Based Strategy Integrated with Molecular Docking and In Vitro Experimental Validation to Explore the Underlying Mechanism of Fangji Huangqi Decoction in Treating Rheumatoid Arthritis

Zhang,

Guo,

et al. 2024

ACS Omega

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Fangji Huangqi decoction (FHD), as a classic traditional Chinese medicine formula, has been clinically proven effective against rheumatoid arthritis (RA), yet its therapeutic mechanism remains unclear. This study employed network pharmacology and molecular docking methods to explore the major active components, biological targets, and signaling pathways of FHD. Subsequently, lipopolysaccharide (LPS)-stimulated RAW264.7 cells were used as the in vitro model to validate the modulating effects of FHD on molecules/inflammatory mediators using various biomedical techniques/kits such as MTT assay, Griess reagents, flow cytometry, RT-qPCR, and immunoblotting. Network pharmacology analyses indicated a total of 20 major active components and 30 core biological targets of FHD against RA. Pathway enrichment analyses demonstrated the involvement of mitogen-activated protein kinase (MAPK) signaling pathways in the efficacy of the formula. Furthermore, experimental evidence demonstrated that FHD dose-dependently and significantly inhibited the productions of nitric oxide (NO) and reactive oxygen species; lowered the mRNA expression levels of proinflammatory mediators including iNOS, COX-2, TNF-α, ΙL-1β, and IL-6; decreased protein levels of the phosphorylated forms of p38, ERK, JNK, and NF-κB p65. Additionally, the results of molecular docking showed that tetrandrine, licochalcone A, oxonantenine, isorhamnetin, and kaempferol in FHD exerted the potent capability of binding to target molecules in the focused signaling pathway, probably being the potential effective substances for FHD. Our network pharmacology study integrated with cellular validation has elucidated that FHD exerts downregulating effects of the MAPK and NF-κB signaling pathway, ultimately leading to inhibitory effects on the productions of proinflammatory mediators in LPS-stimulated RAW264.7 cells. This work comprehensively demonstrated the effective substances, key targets, and signaling pathways involved in the anti-RA effects of the formula, and these findings provide a further understanding of the underlying mechanism of FHD in managing RA.

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Molecular signaling pathways in doxorubicin-induced nephrotoxicity and potential therapeutic agents

Lu,

Wei,

Gao

et al. 2025

International Immunopharmacology

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Metabolomics combined with serum pharmacochemistry discovering the potential effective compounds of Fangji Huangqi Tang against nephrotic syndrome

Cited by 5 publications

References 45 publications

Hypolipidemic activity and mechanism of active components of Dracocephalum moldavica L. tea

Hypolipidemic activity and mechanism of active components of Dracocephalum moldavica L. tea

Network Pharmacology-Based Strategy Integrated with Molecular Docking and In Vitro Experimental Validation to Explore the Underlying Mechanism of Fangji Huangqi Decoction in Treating Rheumatoid Arthritis

Molecular signaling pathways in doxorubicin-induced nephrotoxicity and potential therapeutic agents

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