Metabolomics dataset of PPAR-pan treated rat liver

Abstract

This article contains mass spectrometry (MS) data investigating small molecule changes as an effect of a triple peroxisome proliferator-activated receptor (PPAR-pan) agonist GW625019 in the liver as described in the manuscript (Ament et al., 2016) [1]. Samples were measured using gas chromatography-mass spectrometry (GC–MS) for total fatty acid content, and liquid chromatography-mass spectrometry (LC–MS) to measure intact lipids, carnitines and selected aqueous metabolites and eicosanoids. Data files comprise of Excel (Microsoft, WA, USA) spreadsheets of identified metabolites and their area ratio values for total fatty acids, carnitines, aqueous metabolites, and eicosanoids where the intensity of the analytes were normalised to the intensity of the internal standard. In the case of open profiling intact lipid data, the Excel file contains area ratio values of retention time and mass to charge ratio pairs; again, the area ratio values were calculated by normalising to the intensity of the internal standard. It should be noted that several metabolic changes are potentially indirect (secondary, tertiary and ensuing changes).

PPAR-pan activation induces hepatic oxidative stress and lipidomic remodelling

“…Hepatic total fatty acids were measured by GC–MS ( Supplementary Data 1 [24] ). For an overview of total fatty acid changes multivariate data analysis was performed and a PLS-DA model was constructed to assess the dose response ( R 2 =73%, Q 2 =45%).…”

“…The effects of liver enlargement and fat mobilisation as detected by microscopy were further investigated using an LC–MS/MS-based open profiling approach to detect phospholipids (PLs) and triglycerides (TGs) ( Supplementary Data 2 [24] ). First PLS-DA was used to gain an overview of the intact lipid changes ( Fig.…”

“…Carnitine and acyl-carnitines were measured by LC–MS/MS to investigate whether the decreased TG concentrations are reflected by increased fatty acid β-oxidation ( Supplementary Data 3 [24] ). Multivariate data analysis was used to generate a PLS-DA model ( R 2 =87%, Q 2 =58%) producing an overview of the changes in carnitine species ( Fig.…”

“…Excessive generation of these metabolites can lead to oxidative stress, DNA damage and eicosanoid production. To study the effects of a discontinued PPAR-pan agonist on oxidative metabolite production the aqueous fractions of liver cell extracts were analysed ( Supplementary Data 4 [24] ). The highest dose group separated strongly form controls ( Fig.…”

“…To determine the inflammatory state of the liver caused by this disruption of lipid homeostasis, a range of eicosanoids were measured in the control and the two highest dose level groups ( Supplementary Data 5 [24] ). A targeted LC–MS/MS approach was used and detected decreases in the concentrations of prostaglandins (PG) PGE2, PGD2, and PGF2 and increases in the concentrations of PGB2 and 15-deoxy PGJ2.…”