Metabolomics evaluation of repeated administration of potassium iodide on adult male rats

Rosique, Clément; Lebsir, Dalila; Benatia, Sheherazade; Guigon, Pierre; Caire-Maurisier, François; Benderitter, Marc; Souidi, Maâmar; Martin, Jean‐Charles

doi:10.1007/s00204-020-02666-w

Cited by 8 publications

(10 citation statements)

References 40 publications

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“…Plasma and urine samples were analyzed as described [ 9 ] on two different columns in LC-HRMS: C18 and HILIC. In order to broaden metabolite coverage, the acquisition was operated both in positive and negative ionization mode for each chromatographic condition.…”

Section: Methodsmentioning

confidence: 99%

Plasma and Urinary Amino Acid-Derived Catabolites as Potential Biomarkers of Protein and Amino Acid Deficiency in Rats

et al. 2021

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Objective: Dietary intakes must cover protein and essential amino acid (EAA) requirements. For this purpose, different methods have been developed such as the nitrogen balance method, factorial method, or AA tracer studies. However, these methods are either invasive or imprecise, and the Food and Agriculture Organization of the United Nations (FAO, 2013) recommends new methods and, in particular, metabolomics. The aim of this study is to determine total protein/EAA requirement in the plasma and urine of growing rats. Methods: 36 weanling rats were fed with diets containing 3, 5, 8, 12, 15, and 20% protein for 3 weeks. During experimentation, urine was collected using metabolic cages, and blood from the portal vein and vena was taken at the end of the experiment. Metabolomics analyses were performed using LC-MS, and the data were analyzed with a multivariate analysis model, partial least Squares (PLS) regression, and independent component-discriminant analysis (ICDA). Each discriminant metabolite identified by PLS or ICDA was tested by one-way ANOVA to evaluate the effect of diet. Results: PLS and ICDA allowed us to identify discriminating metabolites between different diet groups. Protein deficiency led to an increase in the AA catabolism enzyme systems inducing the production of breakdown metabolites in the plasma and urine. Conclusion: These results indicate that metabolites are specific for the state of EAA deficiency and sufficiency. Some types of biomarkers such as AA degradation metabolites appear to be specific candidates for protein/EAA requirement.

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Section: Methodsmentioning

confidence: 99%

Plasma and Urinary Amino Acid-Derived Catabolites as Potential Biomarkers of Protein and Amino Acid Deficiency in Rats

et al. 2021

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“…In this sense, the connection between gut microbiota and metal(-loid)s has been explored in mice after arsenic [31][32][33][34][35][36], mercury [37][38][39], copper [38,40], cadmium [41][42][43][44][45], lead [40,41,46,47], iodine [48] and selenium [17,18,49] exposure. Rats have been used as model organisms to assess the interaction of gut microbiota with arsenic [50,51], mercury [52], copper [53,54], cadmium [50,52], iodine [55], manganese [56], lead [57,58], and selenium [59]. Human gut microbiota have been linked to arsenic [60][61][62], mercury [63,64], heavy metals [57,58] and lead [65].…”

Section: Interactionsmentioning

confidence: 99%

“…while lead has been measured in urine [65] serum and the liver [72] and cadmium has been measured in serum [42,73], plasma [74], the liver [43,44,[73][74][75][76] whole blood [44,76] and the kidney [44,75,76]. Remarkably, gut microbiota have been linked to the manganese concentration in brain tissue and plasma [56] and to iodine in thyroid tissue, urine and plasma [48,55]. Different selenium supplementation experiments have been carried out to associate gut microbiota and selenium concentrations in serum [19,59],…”

Section: Metals and Metalloproteinsmentioning

confidence: 99%

Omic methodologies for assessing metal(-loid)s-host-microbiota interplay: A review

Ramírez-Acosta

Arias-Borrego

Navarro-Roldán

et al. 2021

Analytica Chimica Acta

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“…These disadvantages can be avoided with the use of metabolomics. Furthermore, metabolism plays a powerful role in a gaining holistic understanding of various biological matrices by comprehensively measuring small-molecule metabolite levels and is used to identify novel and potential metabolite markers, as well as to explore molecular mechanisms and the response of metabolic pathways to different perturbations [20,21].…”

Section: Introductionmentioning

confidence: 99%

Study on the Effect of Different Iodine Intake on Hippocampal Metabolism in Offspring Rats

Zhang

Fan

et al. 2021

Biol Trace Elem Res

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Iodine is an essential trace element in the human body. Severe maternal iodine deficiency during pregnancy leads to obvious intellectual disability in the offspring. The effects of iodine deficiency on brain development have been demonstrated, but there is no clear evidence of the effects of iodine excess on brain development. To clarify the effects of iodine excess on the brain development of offspring and to provide clues to the mechanisms underlying the effects of iodine deficiency and iodine excess on the brain development of offspring. In this study, animal models with different iodine intakes were constructed using potassium iodate (KIO 3 ). The models included four experimental groups (low-iodine group one (LI, 0μg/L iodine), low-iodine group two (LII, 5μg/L iodine), high-iodine group one (HI, 3000μg/L iodine), and high-iodine group two (HII, 10000μg/L iodine)) and one control group (NI, 100μg/L iodine). There were 20 female rats in each group, and 8 offspring were chosen from each group following birth to assess metabolic alterations. The metabolites of subsets of brain hippocampal tissue were profiled by ultra-performance liquid chromatography-linked electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOFMS) and the results were subjected to multivariate data analysis. Differential substances were screened by t test (p<0.05), principal component analysis (PCA), and partial least squares analysis (PLS-DA, VIP>1). The thyroid function of the female rats in the experimental group was abnormally changed. Metabolic analysis showed that the five groups were separated which revealed significant differences in hippocampal tissue metabolism among the five groups of offspring. A total of 12 potential metabolites were identified, with the majority of them being related to amino acid and energy metabolism. These metabolites are involved in various metabolic pathways, are interrelated, and may play a function in brain development. Our study highlights changes in metabolites and metabolic pathways in the brain hippocampus of offspring rats with different iodine intakes compared to controls, revealing new insights into hippocampal metabolism in offspring rats and new relevant targets. Highlights• Findings aid in the understanding of the damaging mechanisms of iodine deficiency and excess.• Both iodine deficiency and iodine excess caused metabolic disorders in the brain hippocampus of the offspring rats. • Discovery of differential metabolites of the effects of different iodine intakes on brain development in offspring.• Metabolic pathways affected by iodine intake include amino acid metabolism, energy metabolism, and purine metabolism.

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Metabolomics evaluation of repeated administration of potassium iodide on adult male rats

Cited by 8 publications

References 40 publications

Plasma and Urinary Amino Acid-Derived Catabolites as Potential Biomarkers of Protein and Amino Acid Deficiency in Rats

Plasma and Urinary Amino Acid-Derived Catabolites as Potential Biomarkers of Protein and Amino Acid Deficiency in Rats

Omic methodologies for assessing metal(-loid)s-host-microbiota interplay: A review

Study on the Effect of Different Iodine Intake on Hippocampal Metabolism in Offspring Rats

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