Metabolomics in Herbal Medicine Research

Shyur, Lie‐Fen; Liu, Chiu-Ping; Chien, Shih‐Chang

doi:10.1002/9783527669882.ch8

Cited by 6 publications

(2 citation statements)

References 77 publications

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“…The whole-pitcher samples excluding the tendril were ground until fine homogenous powders using pestle and mortar prechilled with liquid nitrogen, collected in a Falcon tube, and freeze-dried for 48 h. Dried powder samples (100 mg) were extracted using 200 µL of methanol:chloroform:water (3:1:1) via the sonication-assisted method (Shyur et al, 2013;Rosli et al, 2017). Samples were vortexed, sonicated in a water bath sonicator at room temperature for 15 min with maximum frequency, vortexed again, and then centrifuged at 10,000 g for 10 min.…”

Section: Phytochemical Extractionmentioning

confidence: 99%

UPLC-TOF-MS/MS-Based Metabolomics Analysis Reveals Species-Specific Metabolite Compositions in Pitchers of Nepenthes ampullaria, Nepenthes rafflesiana, and Their Hybrid Nepenthes × hookeriana

et al. 2021

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Hybridization is key to the evolution and diversity of plants in nature. Nepenthaceae comprises a family of diverse tropical carnivorous pitcher plant species with extensive hybridization. However, there is no study to date on the metabolite expression of hybrids in this family. We performed a non-targeted metabolomics analysis of the pitchers of two Nepenthes species with different dietary habits, namely, the semi-detritivorous N. ampullaria and carnivorous N. rafflesiana with their hybrid (N. × hookeriana) for a comparative study. The whole-pitcher samples were extracted in methanol:chloroform:water (3:1:1) via sonication-assisted extraction and analyzed using ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOF-MS) followed by data analysis to profile chemical compositions. A total of 1,441 metabolite features were profiled from the three species in which 43.3% of features in the hybrid samples were not found in either of its parents. The partial least squares discriminant analysis (PLS-DA) found 324 metabolite features with variable in projection (VIP) values greater than one in which 55 features were statistically significant. This showed that the hybrid is closer to N. rafflesiana, which is consistent to the previous study on gene and protein expressions. A total of 105 metabolites were putatively identified with manual searches using public metabolite databases. Phenols were detected to be the most abundant secondary metabolites due to a high flavonoid content, especially in N. rafflesiana. The most abundant feature 476.3s:449.102 was found to be the most significant VIP for distinguishing between the three species as a chemical marker. This is the first study comparing metabolites in the carnivory organs of different Nepenthes species with comprehensive profiling and putative identification. The differential metabolite compositions in the pitchers of different species might have ecological implications with the hybrid showing intermediate phenotype between the parents as well as manifesting unique metabolites. However, there is no clear evidence of metabolites related to the differences in dietary habits between the hybrid and the two parent species.

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Section: Phytochemical Extractionmentioning

confidence: 99%

UPLC-TOF-MS/MS-Based Metabolomics Analysis Reveals Species-Specific Metabolite Compositions in Pitchers of Nepenthes ampullaria, Nepenthes rafflesiana, and Their Hybrid Nepenthes × hookeriana

et al. 2021

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“…Quality control and the discovery of active components are considered critical for modernizing traditional herbal medicines [149]. Pertinent quality control systems in the phytomedicine industries are necessary to distinguish any potential toxic adulterants from the raw plant materials and achieve consistency in the desired doses of active components in different batches of the product [150].…”

Section: Metabolomics Approach In the Quality Control And Safety Evaluation Of Herbal Exposurementioning

confidence: 99%

Unravelling the Anticancer Mechanisms of Traditional Herbal Medicines with Metabolomics

Oyenihi

Erhabor

et al. 2021

Molecules

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Metabolite profiling of cancer cells presents many opportunities for anticancer drug discovery. The Chinese, Indian, and African flora, in particular, offers a diverse source of anticancer therapeutics as documented in traditional folklores. In-depth scientific information relating to mechanisms of action, quality control, and safety profile will promote their extensive usage in cancer therapy. Metabolomics may be a more holistic strategy to gain valuable insights into the anticancer mechanisms of action of plants but this has remained largely unexplored. This review, therefore, presents the available metabolomics studies on the anticancer effects of herbal medicines commonly used in Africa and Asia. In addition, we present some scientifically understudied ‘candidate plants’ for cancer metabolomics studies and highlight the relevance of metabolomics in addressing other challenges facing the drug development of anticancer herbs. Finally, we discussed the challenges of using metabolomics to uncover the underlying mechanisms of potential anticancer herbs and the progress made in this regard.

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Role of Mass Spectrometry in Modern Herbal Drug Research

Kumar

Madhusudanan

2020

Biophysical and Computational Tools in Drug Discovery

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Metabolomics in Herbal Medicine Research

Cited by 6 publications

References 77 publications

UPLC-TOF-MS/MS-Based Metabolomics Analysis Reveals Species-Specific Metabolite Compositions in Pitchers of Nepenthes ampullaria, Nepenthes rafflesiana, and Their Hybrid Nepenthes × hookeriana

UPLC-TOF-MS/MS-Based Metabolomics Analysis Reveals Species-Specific Metabolite Compositions in Pitchers of Nepenthes ampullaria, Nepenthes rafflesiana, and Their Hybrid Nepenthes × hookeriana

Unravelling the Anticancer Mechanisms of Traditional Herbal Medicines with Metabolomics

Role of Mass Spectrometry in Modern Herbal Drug Research

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