Metabolomics of forage plants: a review

Rasmussen, Susanne; Parsons, A. J.; Jones, Chris S.

doi:10.1093/aob/mcs023

Cited by 52 publications

(38 citation statements)

References 146 publications

(170 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are important trade-offs between stress adaptation and yield in crops depending on how well cultivars cope with changing environment. In drought tolerance, the most typical traits are overaccumulation of carbohydrates and aminoacids but also changes in phenylpropanoids leading to differential flavonoid profiles [139]. This is agreement with previous findings where higher concentrations of phenylpropanoids such as caffeoylquinic acid and phenylalanine were found in tolerant model plant genotypes whereas sinapic acid and flavonoids such as quercetin were higher in sensitive species [140].…”

Section: Effect Of Abiotic Stress On Plant Biochemistry: Metabolitsupporting

confidence: 90%

Metabolomics as a Tool to Investigate Abiotic Stress Tolerance in Plants

Arbona

Manzi²,

Ollas

2013

IJMS

542

316

View full text Add to dashboard Cite

Metabolites reflect the integration of gene expression, protein interaction and other different regulatory processes and are therefore closer to the phenotype than mRNA transcripts or proteins alone. Amongst all –omics technologies, metabolomics is the most transversal and can be applied to different organisms with little or no modifications. It has been successfully applied to the study of molecular phenotypes of plants in response to abiotic stress in order to find particular patterns associated to stress tolerance. These studies have highlighted the essential involvement of primary metabolites: sugars, amino acids and Krebs cycle intermediates as direct markers of photosynthetic dysfunction as well as effectors of osmotic readjustment. On the contrary, secondary metabolites are more specific of genera and species and respond to particular stress conditions as antioxidants, Reactive Oxygen Species (ROS) scavengers, coenzymes, UV and excess radiation screen and also as regulatory molecules. In addition, the induction of secondary metabolites by several abiotic stress conditions could also be an effective mechanism of cross-protection against biotic threats, providing a link between abiotic and biotic stress responses. Moreover, the presence/absence and relative accumulation of certain metabolites along with gene expression data provides accurate markers (mQTL or MWAS) for tolerant crop selection in breeding programs.

show abstract

Section: Effect Of Abiotic Stress On Plant Biochemistry: Metabolitsupporting

confidence: 90%

Metabolomics as a Tool to Investigate Abiotic Stress Tolerance in Plants

Arbona

Manzi²,

Ollas

2013

IJMS

542

316

View full text Add to dashboard Cite

show abstract

“…The need for more research to identify robust metabolic traits and pathways relating to drought tolerance in forage grasses through integration of metabolomic and transcriptomic data have been emphasized in reviews [85]. From our study it was evident that endophyte can affect tall fescue plant metabolism, in response to water deficit stress.…”

Section: Discussionmentioning

confidence: 72%

Tall fescue endophyte effects on tolerance to water-deficit stress

et al. 2013

View full text Add to dashboard Cite

BackgroundThe endophytic fungus, Neotyphodium coenophialum, can enhance drought tolerance of its host grass, tall fescue. To investigate endophyte effects on plant responses to acute water deficit stress, we did comprehensive profiling of plant metabolite levels in both shoot and root tissues of genetically identical clone pairs of tall fescue with endophyte (E+) and without endophyte (E-) in response to direct water deficit stress. The E- clones were generated by treating E+ plants with fungicide and selectively propagating single tillers. In time course studies on the E+ and E- clones, water was withheld from 0 to 5 days, during which levels of free sugars, sugar alcohols, and amino acids were determined, as were levels of some major fungal metabolites.ResultsAfter 2–3 days of withholding water, survival and tillering of re-watered plants was significantly greater for E+ than E- clones. Within two to three days of withholding water, significant endophyte effects on metabolites manifested as higher levels of free glucose, fructose, trehalose, sugar alcohols, proline and glutamic acid in shoots and roots. The fungal metabolites, mannitol and loline alkaloids, also significantly increased with water deficit.ConclusionsOur results suggest that symbiotic N. coenophialum aids in survival and recovery of tall fescue plants from water deficit, and acts in part by inducing rapid accumulation of these compatible solutes soon after imposition of stress.

show abstract

“…This enzyme acts in the phenylpropanoid biosynthesis, pathway associated with drought tolerance mechanisms. The absence of this protein could reduce the level of flavonoids, secondary metabolites involved in the response to different types of stress, which are synthesized from phenylpropanoids (Rasmussen et al, 2012). The gene LOC_Os01g74470.1 encodes a protein of the ABC (ATP-binding cassette) family, which is related to membrane transport, and is involved in secreting pheromones, regulating mitochondrial function, vacuolar detoxification, drug resistance, and stress adaptation (Rea, 2007).…”

Section: Rt-qpcr Gene Expression Validationmentioning

confidence: 99%

Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa)

et al. 2015

View full text Add to dashboard Cite

ABSTRACT. Gene expression related to drought response in the leaf tissues of two Brazilian upland cultivars, the drought-tolerant Douradão and the drought-sensitive Primavera, was analyzed. RNAseq identified 27,618 transcripts in the Douradão cultivar, with 24,090 (87.2%) homologous to the rice database, and 27,221 transcripts in the Primavera cultivar, with 23,663 (86.9%) homologous to the rice database. Gene-expression analysis between control and water-deficient treatments revealed 493 and 1154 differentially expressed genes in Douradão and Primavera cultivars, respectively. Genes exclusively expressed under drought were identified for Douradão, including two genes of particular interest coding for the protein peroxidase precursor, which is involved in three distinct metabolic pathways. Comparisons between the two drought-exposed cultivars revealed 2314 genes were R.D.D. Silveira et al. 8182©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8181-8200 (2015) differentially expressed (978 upregulated, 1336 downregulated in Douradão). Six genes distributed across 4 different transcription factor families (bHLH, MYB, NAC, and WRKY) were identified, all of which were upregulated in Douradão compared to Primavera during drought. Most of the genes identified in Douradão activate metabolic pathways responsible for production of secondary metabolites and genes coding for enzymatically active signaling receptors. Quantitative PCR validation showed that most gene expression was in agreement with computational prediction of these transcripts. The transcripts identified here will define molecular markers for identification of Cisacting elements to search for allelic variants of these genes through analysis of polymorphic SNPs in GenBank accessions of upland rice, aiming to develop cultivars with the best combination of these alleles, resulting in materials with high yield potential in the event of drought during the reproductive phase.

show abstract

Metabolomics of forage plants: a review

Cited by 52 publications

References 146 publications

Metabolomics as a Tool to Investigate Abiotic Stress Tolerance in Plants

Metabolomics as a Tool to Investigate Abiotic Stress Tolerance in Plants

Tall fescue endophyte effects on tolerance to water-deficit stress

Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa)

Contact Info

Product

Resources

About