Metabolomics of A rabidopsis Thaliana

Abstract

Since the publication of the complete genome sequence of the model plant Arabidopsis thaliana, the precision annotation of the exact function of each gene has become a major research activity. Metabolomic analysis has much to offer in this endeavour and the small physical size and generation time of Arabidopsis make it a particularly attractive system for large‐scale metabolomic data collection and analysis. In this review, we summarize the known phytochemistry of Arabidopsis, with particular emphasis on recent discoveries that indicate that the complexity of this plant's secondary metabolome is much greater than originally realized. We survey how the different analytical technologies, used in metabolomic analysis, are applied to quantify both primary and secondary metabolites of Arabidopsis. The use of isotope labelling to derive empirical formulae for unknown metabolites is highlighted, as is co‐expression analysis of transcriptome and metabolome data, as both of these technologies are well suited to this species. The application of the various techniques to study the molecular plant physiology, genetics and functional genomics of Arabidopsis is reviewed, with particular reference to the use of mutants and other genetic resources, such as recombinant inbred lines.

Exogenously Applied Rohitukine Inhibits Photosynthetic Processes, Growth and Induces Antioxidant Defense System in Arabidopsis thaliana

“…The metabolome offers better visualization of the changes in the levels of many different metabolites. In recent years, metabolomics has gained considerable attention as a tool for acquiring better insight into the biological processes of organisms [ 82 , 83 , 84 , 85 ]. In an attempt to analyse the changes in metabolite levels caused by rohitukine in A. thaliana , GC–MS-based metabolomics was performed, in which amino acids, carbohydrates, other organic acids, etc., were identified.…”

Quantitative trait loci analysis of glucosinolate, sugar, and organic acid concentrations in Eruca vesicaria subsp. sativa

“…The TCA cycle also produces precursors to amino acids like methionine, which is an important component of aliphatic GSL biosynthesis (Malitsky et al 2008 ). Like A. thaliana , rocket is known to accumulate high organic acid concentrations (Beale and Sussman 2018 ; Bell et al 2017 ) and increased abundances within leaves may be an indicator of metabolic stress (Shi et al 2015 ).…”