Metabolomics of laminae and midvein during leaf senescence and source–sink metabolite management in Brassica napus L. leaves

Clément, Gilles; Moison, Michaël; Soulay, Fabienne; Reisdorf-Cren, Michèle; Masclaux-Daubresse, Céline

doi:10.1093/jxb/erx253

Cited by 48 publications

(41 citation statements)

References 48 publications

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“…For example, in young tobacco leaves, 20% of the total amino acid content is present as proline, while marginal concentrations (2%) are found in mature and senescing leaves (Masclaux et al, 2000). Leaves of Arabidopsis and canola (Brassica napus) plants grown under high-nitrate conditions show higher free amino acid levels and different amino acid compositions than under low nitrate Cl ement et al, 2017). Further, environmental challenges such as drought and salt stress result in amino acid accumulation in leaves as a result of decreased usage for protein synthesis or release from protein degradation (Chaffei et al, 2004;Martinelli et al, 2007;Verbruggen & Hermans, 2008).…”

Section: Amino Acid Storagementioning

confidence: 99%

Source and sink mechanisms of nitrogen transport and use

2017

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Contents Summary35I.Introduction35II.Nitrogen acquisition and assimilation36III.Root‐to‐shoot transport of nitrogen38IV.Nitrogen storage pools in vegetative tissues39V.Nitrogen transport from source leaf to sink40VI.Nitrogen import into sinks42VII.Relationship between source and sink nitrogen transport processes and metabolism43VIII.Regulation of nitrogen transport43IX.Strategies for crop improvement44X.Conclusions46Acknowledgements47References47 Summary Nitrogen is an essential nutrient for plant growth. World‐wide, large quantities of nitrogenous fertilizer are applied to ensure maximum crop productivity. However, nitrogen fertilizer application is expensive and negatively affects the environment, and subsequently human health. A strategy to address this problem is the development of crops that are efficient in acquiring and using nitrogen and that can achieve high seed yields with reduced nitrogen input. This review integrates the current knowledge regarding inorganic and organic nitrogen management at the whole‐plant level, spanning from nitrogen uptake to remobilization and utilization in source and sink organs. Plant partitioning and transient storage of inorganic and organic nitrogen forms are evaluated, as is how they affect nitrogen availability, metabolism and mobilization. Essential functions of nitrogen transporters in source and sink organs and their importance in regulating nitrogen movement in support of metabolism, and vegetative and reproductive growth are assessed. Finally, we discuss recent advances in plant engineering, demonstrating that nitrogen transporters are effective targets to improve crop productivity and nitrogen use efficiency. While inorganic and organic nitrogen transporters were examined separately in these studies, they provide valuable clues about how to successfully combine approaches for future crop engineering.

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Section: Amino Acid Storagementioning

confidence: 99%

Source and sink mechanisms of nitrogen transport and use

2017

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“…The concentrations of free amino acids in leaves are closely related to leaf growth ( Masclaux et al , 2000 ; Havé et al , 2016 ). Increased N supply significantly increases the concentration of free amino acids in leaves ( Lemaître et al , 2008 ; Clément et al , 2017 ). Proline, glutamine, and arginine can be used as N storage sources, and their use varies by species ( Nordin and Näsholm, 1997 ; Lemaître et al , 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Storage nitrogen co-ordinates leaf expansion and photosynthetic capacity in winter oilseed rape

Liu

Ren

White

et al. 2018

Journal of Experimental Botany

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“…Developmental leaf senescence correlated with the decrease in Ser, Gly, Glu, Ala, Asp, Pro and Val contents in Arabidopsis [21] and in tobacco [53]. A recent analysis of the metabolic fingerprint associated with leaves having a different sink/source status in winter oilseed rape genotype Darmor bzh showed a correlation between the decrease in Glu and Asp contents in the acquisition of source status by leaves [54]. Since we performed our analysis on a winter oilseed rape genotype Aviso, our results suggested that metabolic fingerprint could be an interesting tool to separate different genotypes.…”

Section: Discussionmentioning

confidence: 92%

Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (Brassica napus L.)

et al. 2020

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Nitrogen remobilization processes from source to sink tissues in plants are determinant for seed yield and their implementation results in a complete reorganization of the primary metabolism during sink/source transition. Here, we decided to characterize the impact of the sink/source balance on amino acid metabolism in the leaves of winter oilseed rape grown at the vegetative stage. We combined a quantitative metabolomics approach with an instationary 15N-labeling experiment by using [15N]L-glycine as a metabolic probe on leaf ranks with a gradual increase in their source status. We showed that the acquisition of the source status by leaves was specifically accompanied by a decrease in asparagine, glutamine, proline and S-methyl-l-cysteine sulphoxide contents and an increase in valine and threonine contents. Dynamic analysis of 15N enrichment and concentration of amino acids revealed gradual changes in the dynamics of amino acid metabolism with respect to the sink/source status of leaf ranks. Notably, nitrogen assimilation into valine, threonine and proline were all decreased in source leaves compared to sink leaves. Overall, our results suggested a reduction in de novo amino acid biosynthesis during sink/source transition at the vegetative stage.

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Metabolomics of laminae and midvein during leaf senescence and source–sink metabolite management in Brassica napus L. leaves

Abstract: Metabolomics of leaf laminae and veins during ageing reveal tissue specificities and different senescence programmes.

Cited by 48 publications

References 48 publications

Source and sink mechanisms of nitrogen transport and use

Source and sink mechanisms of nitrogen transport and use

Storage nitrogen co-ordinates leaf expansion and photosynthetic capacity in winter oilseed rape

Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (Brassica napus L.)

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