Metabolomics of Ulva lactuca Linnaeus (Chlorophyta) exposed to oil fuels: Fourier transform infrared spectroscopy and multivariate analysis as tools for metabolic fingerprint

Pilatti, Fernanda Kokowicz; Ramlov, Fernanda; Schmidt, Éder C.; Costa, Christopher; Oliveira, Eva Regina; Bauer, Cláudia Marlene; Rocha, Miguel; Bouzon, Zenilda L.; Maraschin, Marcelo

doi:10.1016/j.marpolbul.2016.11.006

Cited by 18 publications

(11 citation statements)

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“…We use FTIR and multivariate analysis to discriminate metabolic profiles of Ulva lactuca after in vitro exposure to diesel oil and gasoline. As a result, Pilatti et al (2017) observed that both PCA and HCA performed on entire mid-infrared spectral window were able to discriminate diesel oil-exposed thalli from the gasoline-exposed ones. In addition to this, HCA performed in the spectral window related to protein absorbance (1700-1500 cm -1 ) allowed the best discrimination between samples exposed to gasoline and diesel oil.…”

Section: Discussionmentioning

confidence: 95%

“…Combination of FTIR and multivariate analysis presents an important tool in metabolic profiles discrimination from Ulva lactuca exposed to diesel oil and gasoline. Pilatti et al (2017) observed that spectral window related to protein absorbance (1700-1500 cm−1) enabled the best discrimination between gasoline-exposed samples regarding exposure time, and between diesel oil-exposed samples.…”

Section: Introductionmentioning

confidence: 93%

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Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta) responses to gasoline short-term exposure: biochemical and cellular alterations

et al. 2019

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Presence of toxic compounds in marine coastal waters has increased exponentially since Industrial Revolution. In this way, we aimed to evaluate biochemical and physiological changes occurring within Hypnea musciformis after short-term exposure to gasoline. Hypnea musciformis was cultivated without gasoline and then exposed to various concentrations of it (0.001 %-1.0 %, v/v) for periods of 30 min, 1 h, 12 h and 24 h. A Pricncipal Compound Analysis of UV-vis spectral window (200-700 nm) was able to discriminate gasoline-exposed samples according to both exposure time and gasoline concentration. Changes in carotenoid profile composition were observed. Decreased carotenoid content was associated to gasoline exposure time, being lutein and trans-β-carotene the major compounds found. Higher gasoline concentrations negatively interfered with phenolic compounds accumulation. In addition, increased gasoline concentrations corresponded to decreased intracellular starch grains content as well as increased its deposition on cell wall external surface. Data obtained allow us to conclude that gasoline can damage Hypnea musciformis physiology and cell morphology. This is important, considering Hypnea musciformis carotenoids and phenolics are potential biomarkers of environmental stress investigated, as well as its increased cell wall thickness to avoid gasoline diffusion.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 93%

Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta) responses to gasoline short-term exposure: biochemical and cellular alterations

et al. 2019

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“…The presence of phenol in wastewater might influence the chemical composition of activated sludge. Other studies concerning investigation of marine plant exposed to petrol and diesel changes in metabolic profile revealed that there were visible changes in FT-IR spectra of plants exposed and non-exposed to xenobiotics (Pilatti et al 2017 ).…”

Section: Resultsmentioning

confidence: 99%

“…Performing FT-IR analysis of samples provides useful indicators to characterize changes in organic matters, without the need of extraction procedures (Bernier et al 2013 ). This analytical approach is simple, cheap and efficient in applications aimed to determine changes in investigated media (Pilatti et al 2017 ).…”

Section: Resultsmentioning

confidence: 99%

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Qualitative analysis of activated sludge using FT-IR technique

et al. 2018

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The ability to measure and control the composition of activated sludge is an important issue, aiming at evaluating the effectiveness of changes occurring in the sludge, what determines its usefulness to treat wastewater. In this research, diffuse reflectance infrared Fourier transform (FTIR–DRIFT) technique was used, which relies on measuring the reflectance of the powdered substance’s surface layer and capturing spectra in range of infrared wave. First, spectra correlation table of the substances mostly occurring in wastewater was developed to assess the main components of the tested samples of activated sludge. The simplest compounds containing functional groups characteristic for particular chemical classes were chosen: peptides (peptone and albumin), fats (glycerin and fatty acids), carbohydrates (glucose and sucrose), nitrogen compounds (NaNO3 and NH4SO4), sulfur compounds (Na2SO4 and Na2S2O3), silicate, etc. The spectra of those substances were captured and characteristic absorption bands for respective bonds in the function groups were assigned. Second, samples of activated sludge from lab-scale membrane bioreactors (MBRs), which purifies petroleum wastewater, were taken. Samples were properly prepared (lyophilization and homogenization) and their spectra were captured. During spectra analysis, previously developed correlation table was used. In obtained spectra of activated sludge, absorption bonds characteristic for amides, peptides, carbohydrates, fats, and aliphatic was identified. The spectra profile of the sludge sample from MBR feed with petroleum wastewater was slightly different from the control MBR sample’s spectra. Intensity of bands in the area characteristic for aliphatic compounds and phenols was clearly higher. This study proves the usefulness of FT-IR technique to observe changes in the chemical composition of activated sludge.

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