Metabolomics profiling of plasma, urine and saliva after short term training in young professional football players in Saudi Arabia

Alzharani, Mansour A.; Alshuwaier, Ghareeb O.; Aljaloud, Khalid Saad; Al‐Tannak, Naser F.; Watson, David

doi:10.1038/s41598-020-75755-6

Cited by 14 publications

(8 citation statements)

References 31 publications

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“…Exercise, however, is known to accelerate fat metabolism, thereby increasing acylcarnitine levels in plasma 69 , 80 . Thus, the decrease of these acylcarnitines in urine and stool in this study might imply that they were used in circulation or as metabolic substrates for muscle activity 81 . This was further confirmed by the nominal increase of acylcarnitines in plasma.…”

Section: Discussionmentioning

confidence: 77%

Effects of exercise on NAFLD using non-targeted metabolomics in adipose tissue, plasma, urine, and stool

Babu

Csader

Männistö

et al. 2022

Sci Rep

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The mechanisms by which exercise benefits patients with non-alcoholic fatty liver disease (NAFLD), the most common liver disease worldwide, remain poorly understood. A non-targeted liquid chromatography-mass spectrometry (LC–MS)-based metabolomics analysis was used to identify metabolic changes associated with NAFLD in humans upon exercise intervention (without diet change) across four different sample types—adipose tissue (AT), plasma, urine, and stool. Altogether, 46 subjects with NAFLD participated in this randomized controlled intervention study. The intervention group (n = 21) performed high-intensity interval training (HIIT) for 12 weeks while the control group (n = 25) kept their sedentary lifestyle. The participants' clinical parameters and metabolic profiles were compared between baseline and endpoint. HIIT significantly decreased fasting plasma glucose concentration (p = 0.027) and waist circumference (p = 0.028); and increased maximum oxygen consumption rate and maximum achieved workload (p < 0.001). HIIT resulted in sample-type-specific metabolite changes, including accumulation of amino acids and their derivatives in AT and plasma, while decreasing in urine and stool. Moreover, many of the metabolite level changes especially in the AT were correlated with the clinical parameters monitored during the intervention. In addition, certain lipids increased in plasma and decreased in the stool. Glyco-conjugated bile acids decreased in AT and urine. The 12-week HIIT exercise intervention has beneficial ameliorating effects in NAFLD subjects on a whole-body level, even without dietary changes and weight loss. The metabolomics analysis applied to the four different sample matrices provided an overall view on several metabolic pathways that had tissue-type specific changes after HIIT intervention in subjects with NAFLD. The results highlight especially the role of AT in responding to the HIIT challenge, and suggest that altered amino acid metabolism in AT might play a critical role in e.g. improving fasting plasma glucose concentration.Trial registration ClinicalTrials.gov (NCT03995056).

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Section: Discussionmentioning

confidence: 77%

Effects of exercise on NAFLD using non-targeted metabolomics in adipose tissue, plasma, urine, and stool

Babu

Csader

Männistö

et al. 2022

Sci Rep

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“…Physical activity plays a key role in well-being and keeps the body fit ( Alzharani et al, 2020 ). Inducing metabolic changes in the whole organism, physical activity, and more precisely training in sports practice, leads to the activation of adaptive responses to establish a new equilibrium with beneficial effects for the entire body and performance ( Magherini et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Modulation of Plasma Proteomic Profile by Regular Training in Male and Female Basketball Players: A Preliminary Study

et al. 2022

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Monitoring fatigue and recovery during training periods contributes to identifying the best training methods to achieve sports performance. To date, little is known about sex-related differences in sports adaptations. The aim of the present study is to identify sex-related sports adaptation proteins in female basketball players and male basketball players using proteomics approach on plasma samples withdrawn from athletes during in-season training period but far from a competition. A cohort of 20 professional basketball players, 10 female (BF) and 10 male (BM), and 20 sedentary male (10 CM) and female (10 CF) as control, of comparable age and BMI, were involved in this study. Protein profiles of plasma samples obtained from BM, BF, CM, and CF were analyzed by two-dimensional electrophoresis (2-DE). Differentially expressed proteins were identified by mass spectrometry. The computational 2-DE gel image analysis pointed out 33 differentially expressed protein spots (ANOVA p-value < 0.05) and differences between male and female basketball players are more evident among the players than controls. The expression profile of 54.5% of the total proteins is affected by sports activity. Furthermore, 14 proteins are differentially expressed in basket female players in comparison with their relative controls while seven are differentially expressed in basket male players in comparison with their controls. In conclusion, we identify in female athletes a reduction in proteins related to transcription regulation, most of these modulate chronic inflammation confirming the anti-inflammatory effect of regular training in female muscle metabolism. In male and female athletes, we found a decrease in Transthyretin involved in muscle homeostasis and regeneration and Dermcidin a stress-induced myokine linked to inflammatory and it will be interesting to fully understand the role of its different isoforms in male and female skeletal muscle contraction.

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“…Nevertheless, saliva and urinary metabolome analyses have been employed to investigate performance testing in soccer players [ 15 ], pre- and post-match play [ 16 ], and during a season to examine fatigue [ 17 ] or physical load [ 18 ] as well as in basketball throughout match play [ 19 ]. Recent work in youth soccer analyzed blood plasma, urine, and saliva to observe the effect of short-term physical activity upon the metabolome [ 20 ]. In addition to the synchronous analysis of multiple biofluids, it is also crucial to investigate the recovery period beyond 24 h as no exercise metabolomics research has examined this to date [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“Fuel for the Damage Induced”: Untargeted Metabolomics in Elite Rugby Union Match Play

et al. 2021

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The metabolic perturbations caused by competitive rugby are not well characterized. Our aim is to utilize untargeted metabolomics to develop appropriate interventions, based on the metabolic fluctuations that occur in response to this collision-based team sport. Seven members of an English Premiership rugby squad consented to provide blood, urine, and saliva samples daily, over a competitive week including gameday (GD), with physical demands and dietary intake also recorded. Sample collection, processing and statistical analysis were performed in accordance with best practice set out by the metabolomics standards initiative employing 700 MHz NMR spectroscopy. Univariate and multivariate statistical analysis were employed to reveal the acute energy needs of this high intensity sport are met via glycolysis, the TCA cycle and gluconeogenesis. The recovery period after cessation of match play and prior to training recommencing sees a re-entry to gluconeogenesis, coupled with markers of oxidative stress, structural protein degradation, and reduced fatty acid metabolism. This novel insight leads us to propose that effective recovery from muscle damaging collisions is dependent upon the availability of glucose. An adjustment in the periodisation of carbohydrate to increase GD+1 provision may prevent the oxidation of amino acids which may also be crucial to allay markers of structural tissue degradation. Should we expand the ‘Fuel for the work required’ paradigm in collision-based team sports to include ‘Fuel for the damage induced’?

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Metabolomics profiling of plasma, urine and saliva after short term training in young professional football players in Saudi Arabia

Cited by 14 publications

References 31 publications

Effects of exercise on NAFLD using non-targeted metabolomics in adipose tissue, plasma, urine, and stool

Effects of exercise on NAFLD using non-targeted metabolomics in adipose tissue, plasma, urine, and stool

Modulation of Plasma Proteomic Profile by Regular Training in Male and Female Basketball Players: A Preliminary Study

“Fuel for the Damage Induced”: Untargeted Metabolomics in Elite Rugby Union Match Play

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