Metabolomics reveals impact of seven functional foods on metabolic pathways in a gut microbiota model

Farag, Mohamed A.; Abdelwareth, Amr; Sallam, Ibrahim E.; Shorbagi, Mohamed; Jehmlich, Nico; Fritz-Wallace, Katarina; Schäpe, Stephanie Serena; Rolle-Kampczyk, Ulrike; Ehrlich, Anja; Wessjohann, Ludger A.; Bergen, Martin von

doi:10.1016/j.jare.2020.01.001

Cited by 86 publications

(53 citation statements)

References 77 publications

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“…High levels of glucose have been reported in extracts of macroscopically uninvolved colonic mucosa of IBD patients [ 72 ], and also in other biological specimens including fecal extracts in patients with ulcerative colitis [ 73 ]. In support of such a hypothesis, energy-related metabolites in colon appear to be crucial for homeostasis of the gut microbiome and intestinal cells [ 74 , 75 ], and to suggest that increased glucose level in serum samples of the UC rats may be resulting from the disturbance in the host-microbiome system [ 76 ].…”

Section: Resultsmentioning

confidence: 99%

Protective Mechanism of Acacia saligna Butanol Extract and Its Nano-Formulations against Ulcerative Colitis in Rats as Revealed via Biochemical and Metabolomic Assays

Abdallah

Ammar

Abdelhameed

et al. 2020

Biology

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Ulcerative colitis (UC) is a relapsing inflammatory disease of unknown etiology. The increased risk of cancer in UC patients warrants for the development of novel drug treatments. Herein, this work concerns with the investigation of the protective effects of Acacia saligna butanol extract (ASBE) and its nanoformulations on UC in a rat model and its underlying mechanism. Colitis was induced by slow intrarectal infusion of 2 mL of 4% (v/v in 0.9% saline) acetic acid. Colon samples were evaluated macroscopically, microscopically, and assayed for pro-inflammatory cytokine levels. To monitor associated metabolic changes in acetic acid-induced UC model, serum samples were analyzed for primary metabolites using GC–MS followed by multivariate data analyses. Treatment with ASBE attenuated acetic acid-induced UC as revealed by reduction of colon weight, ulcer area, and ulcer index. ASBE treatment also reduced Cyclooxygenase-2 (COX-2), Prostaglandin E2 (PGE2) & Interleukin-1β (IL-1β) levels in the inflamed colon. The nano-formulation of ASBE showed better protection than the crude extract against ulcer indices, increased PGE2 production, and histopathological alterations such as intestinal mucosal lesions and inflammatory infiltration. Distinct metabolite changes were recorded in colitis rats including a decrease in oleamide and arachidonic acid along with increased levels of lactic acid, fructose, and pyroglutamic acid. Treatment with nano extract restored metabolite levels to normal and suggests that cytokine levels were regulated by nano extract in UC. Conclusion: ASBE nano extract mitigated against acetic acid-induced colitis in rats, and the underlying mechanism could be attributed to the modulatory effects of ASBE on the inflammatory cascades. The applicability of metabolomics developed in this rat model seems to be crucial for evaluating the anti-inflammatory mechanisms of new therapeutics for acute colitis.

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Section: Resultsmentioning

confidence: 99%

Protective Mechanism of Acacia saligna Butanol Extract and Its Nano-Formulations against Ulcerative Colitis in Rats as Revealed via Biochemical and Metabolomic Assays

Abdallah

Ammar

Abdelhameed

et al. 2020

Biology

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“…Currently, approaches based on omics sciences provide more information on the potential of bioactive compounds, their association, interactions and their possible genetic role. 113 In this sense, omics sciences are showing a plethora of information related to the interaction of tea with the gut microbiota. There is a strong evidence that the gut microbiota provides information about different characteristics, such as geographical location, nutrition or host genetics.…”

Section: New Approaches and Developmentsmentioning

confidence: 99%

“…114 Gut microbiota can regulate the immune system, prevent chronic diseases and affect the metabolism and bioavailability of different compounds. 84,113,114 Meta-analytical methods showed that the consumption of tea, specifically black tea, 114 oolong tea 115 and green tea 116 affected the abundance of gut microbiota in a dose-dependent manner. 116 Green tea polyphenols can modulate the diversity and composition of the gut microbiota.…”

Section: New Approaches and Developmentsmentioning

confidence: 99%

“…116 Green tea polyphenols can modulate the diversity and composition of the gut microbiota. 84,86,97,[113][114][115][116][117][118][119][120][121][122][123] Studies suggest that they promote the growth of specific bacteria, mainly beneficial bacterial species such as Bifidobacterium spp., Lactobacillus spp., Clostridium coccoides, Actinobacteria and Firmicutes, while inhibiting the proliferation of pathogenic bacterial species such as Clostridium perfringens, Clostridium difficile, Bacteroides spp., E. coli O157 and Helicobacter pylori. 84,114,115,117,124 These changes in turn affect the host health.…”

Section: New Approaches and Developmentsmentioning

confidence: 99%

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Green and white teas as health-promoting foods

et al. 2021

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“…Diet can change the composition of feces, which directly alter metabolite profiles [ 65 ]. Moreover, consumed food impacts metabolic pathways of gut microbiota and volatiles produced by them [ 66 ]. Factors related to the collection and handling of fecal samples like post-collection metabolite deterioration, due to exposure to aerobic conditions and ongoing microbial fermentation, also take place [ 67 ].…”

Section: Studies On Colorectal Cancer Metabolic Biomarkersmentioning

confidence: 99%

A Review of GC-Based Analysis of Non-Invasive Biomarkers of Colorectal Cancer and Related Pathways

Monedeiro

Monedeiro-Milanowski

Ligor

et al. 2020

JCM

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Colorectal cancer (CRC) is the third most commonly diagnosed cancer in the world. In Europe, it is the second most common cause of cancer-related deaths. With the advent of metabolomics approaches, studies regarding the investigation of metabolite profiles related to CRC have been conducted, aiming to serve as a tool for early diagnosis. In order to provide further information about the current status of this field of research, 21 studies were systematically reviewed, regarding their main findings and analytical aspects. A special focus was given to the employment of matrices obtained non-invasively and the use of gas chromatography as the analytical platform. The relationship between the reported volatile and non-volatile biomarkers and CRC-related metabolic alterations was also explored, demonstrating that many of these metabolites are connected with biochemical pathways proven to be involved in carcinogenesis. The most commonly reported CRC indicators were hydrocarbons, aldehydes, amino acids and short-chain fatty acids. These potential biomarkers can be associated with both human and bacterial pathways and the analysis based on such species has the potential to be applied in the clinical practice as a low-cost screening method.

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Metabolomics reveals impact of seven functional foods on metabolic pathways in a gut microbiota model

Cited by 86 publications

References 77 publications

Protective Mechanism of Acacia saligna Butanol Extract and Its Nano-Formulations against Ulcerative Colitis in Rats as Revealed via Biochemical and Metabolomic Assays

Protective Mechanism of Acacia saligna Butanol Extract and Its Nano-Formulations against Ulcerative Colitis in Rats as Revealed via Biochemical and Metabolomic Assays

Green and white teas as health-promoting foods

A Review of GC-Based Analysis of Non-Invasive Biomarkers of Colorectal Cancer and Related Pathways

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