Metabolomics study of Buyang Huanwu Tang Decoction in ischemic stroke mice by 1H NMR

Chen, Hong-Jhang; Shen, Yung Kang; Lin, Chin‐Yu; Tsai, Keng‐Chang; Lu, Chung‐Kuang; Shen, Chien‐Chang; Lin, Yun-Lian

doi:10.1007/s11306-011-0394-0

Cited by 22 publications

(20 citation statements)

References 36 publications

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“…Taurine, an inhibitory amino acid, has multiple biofunctions in the central nervous system, including osmoregulation, neuromodulation and neuroprotection [31]. The increased serum level of taurine agree with the results of previous studies [17,32], and are probably due to its leakage from damaged cells in response to oxidative insults. Moreover, the higher glycerol content present in brain also reflected the ROS-induced degradation of phospholipids in the cellular membranes, because glycerol is an end product of membrane phospholipid hydrolysis [33].…”

Section: Discussionsupporting

confidence: 87%

Exploring the neuroprotective effects of ginkgolides injection in a rodent model of cerebral ischemia–reperfusion injury by GC–MS based metabolomic profiling

Geng

Feng

et al. 2017

Journal of Pharmaceutical and Biomedical Analysis

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Section: Discussionsupporting

confidence: 87%

Exploring the neuroprotective effects of ginkgolides injection in a rodent model of cerebral ischemia–reperfusion injury by GC–MS based metabolomic profiling

Geng

Feng

et al. 2017

Journal of Pharmaceutical and Biomedical Analysis

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“…The Cr-PCr system, through the creatine kinase (CK) reaction, played crucial roles in maintaining a constant ATP level (Chen et al, 2012). With the improved energy supply in F9 group, the other energy production means were no longer necessary as exemplified by the elevated Cr level, and reduced ketone body (3-HB) level in brains of F9 rats, as compared with those in the MCAO rats.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Huang-Lian-Jie-Du-Decoction for Ischemic Stroke Treatment and Mechanistic Study by Metabolomic Profiling and Network Analysis

et al. 2017

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Optimal drug proportions and mechanism deciphering of multicomponent drugs are critical for developing novel therapies to cope with complex diseases, such as stroke. In the present study, orthogonal experimental design was applied to explore the optimal proportion of the four component herbs in Huang-Lian-Jie-Du-Decoction (HLJDD) on the treatment of ischemic stroke. The treatment efficacies and mechanisms were assessed using global and amino acids (AAs) targeted metabolomics, as well as correlation network analysis. The global NMR metabolomics results revealed that AAs metabolism was significantly perturbed in middle cerebral artery occlusion rats. The levels of 23 endogenous AAs were then subjected to HPLC-QTOF-MS/MS analysis. These results complemented with neurobehavioral evaluations, cerebral infarct assessments, biochemical evaluations, histological inspections and immunohistochemistry observations strongly demonstrated that HLJDD with optimal proportion of 6 (Rhizoma coptidis): 4 (Radix scutellariae): 1 (Cortex phellodendr): 3 (Fructus Gardeniae) had the best efficacy on ischemic stroke, which could be ascribed to its modulation on AA metabolism. This integrated metabolomics approach showed the potential and applicable in deciphering the complex mechanisms of traditional Chinese medicine formulae on the treatment of complicated diseases, which provided new means to assess the treatment effects of herb combinations and to further development of drugs or therapies based on these formulae.

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“…Our previous genomics study reported that BHD protects mice against ischemic stroke by downregulating inflammation-, apoptosis-, angiogenesis-, and blood coagulation-related genes, as well as upregulating neurogenesis- and the nervous system’s development-related genes [ 18 ]. Furthermore, an NMR-based metabolomics study demonstrated that BHD ameliorates the aberrant metabolism of the brain in CIR mice [ 19 ]. Hao et al, reported that BHD improves the neurological deficit and seems generally safe in patients with acute ischemic stroke, based on a clinical trial performed in China [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Multiplex Brain Proteomic Analysis Revealed the Molecular Therapeutic Effects of Buyang Huanwu Decoction on Cerebral Ischemic Stroke Mice

et al. 2015

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Stroke is the second-leading cause of death worldwide, and tissue plasminogen activator (TPA) is the only drug used for a limited group of stroke patients in the acute phase. Buyang Huanwu Decoction (BHD), a traditional Chinese medicine prescription, has long been used for improving neurological functional recovery in stroke. In this study, we characterized the therapeutic effect of TPA and BHD in a cerebral ischemia/reperfusion (CIR) injury mouse model using multiplex proteomics approach. After the iTRAQ-based proteomics analysis, 1310 proteins were identified from the mouse brain with <1% false discovery rate. Among them, 877 quantitative proteins, 10.26% (90/877), 1.71% (15/877), and 2.62% (23/877) of the proteins was significantly changed in the CIR, BHD treatment, and TPA treatment, respectively. Functional categorization analysis showed that BHD treatment preserved the integrity of the blood–brain barrier (BBB) (Alb, Fga, and Trf), suppressed excitotoxicity (Grm5, Gnai, and Gdi), and enhanced energy metabolism (Bdh), thereby revealing its multiple effects on ischemic stroke mice. Moreover, the neurogenesis marker doublecortin was upregulated, and the activity of glycogen synthase kinase 3 (GSK-3) and Tau was inhibited, which represented the neuroprotective effects. However, TPA treatment deteriorated BBB breakdown. This study highlights the potential of BHD in clinical applications for ischemic stroke.

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Metabolomics study of Buyang Huanwu Tang Decoction in ischemic stroke mice by 1H NMR

Cited by 22 publications

References 36 publications

Exploring the neuroprotective effects of ginkgolides injection in a rodent model of cerebral ischemia–reperfusion injury by GC–MS based metabolomic profiling

Exploring the neuroprotective effects of ginkgolides injection in a rodent model of cerebral ischemia–reperfusion injury by GC–MS based metabolomic profiling

Optimization of Huang-Lian-Jie-Du-Decoction for Ischemic Stroke Treatment and Mechanistic Study by Metabolomic Profiling and Network Analysis

Multiplex Brain Proteomic Analysis Revealed the Molecular Therapeutic Effects of Buyang Huanwu Decoction on Cerebral Ischemic Stroke Mice

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