Metabolomics Study of Different Germplasm Resources for Three Polygonatum Species Using UPLC-Q-TOF-MS/MS

Abstract

Rhizomes of the Polygonatum species are well-known in traditional Chinese medicine. The 2020 edition of Chinese Pharmacopoeia includes three different species that possess different pharmacological effects. Due to the lack of standardized discriminant compounds there has often been inadvertently incorrect prescriptions given for these medicines, resulting in serious consequences. Therefore, it is critical to accurately distinguish these herbal Polygonatum species. For this study, UPLC-Q-TOF-MS/MS based metabolomics was employed for the first time to discriminate between three Polygonatum species. Partial least squares discriminant analysis (PLS-DA) models were utilized to select the potential candidate discriminant compounds, after which MS/MS fragmentation patterns were used to identify them. Meanwhile, metabolic correlations were identified using the R language package corrplot, and the distribution of various metabolites was analyzed by box plot and the Z-score graph. As a result, we found that adenosine, sucrose, and pyroglutamic acid were suitable for the identification of different Polygonatum species. In conclusion, this study articulates how various herbal Polygonatum species might be more accurately and efficiently distinguished.

Application of proteomics to determine the mechanism of ozone on sweet cherries (Prunus avium L.) by time-series analysis

“…The level of weight loss, rotting rate and TSS was highest in the CK group and approximately twofold higher than the ozone treatment group. However, the other indicators of SOD, POD, firmness were higher in the ozone treatment, this result is consistent with previous literature reports ( Wang et al., 2022 ). These results suggest a relationship between ozone treatment and traits, ozone can significantly affect the parameters of cherry.…”

Metabolic Composition and Quality Traits of Polygonatum cyrtonema Hua from Different Germplasms and Age Sections Based on Widely Targeted Metabolomics Analysis

“…[ 21 ]. Although researchers have reported many metabolic components in Polygonatum species, previous studies have focused on only a few metabolites, such as polysaccharides, saponins, and flavonoids [ 9 , 10 ]. The metabolite differences in rhizomes among different germplasms and different age sections of P. cyrtonema have not yet been studied in depth.…”

“…Related studies have suggested that differences in the metabolic composition between varieties of the same species may result in species with different qualities and characteristics [ 7 , 8 ]. Some variation in the medicinal composition of Polygonatum species from different germplasms has been reported [ 9 , 10 ], and significant differences in the medicinal composition of Polygonatum species from the same germplasms have been found among different age sections and varying cultivation environments [ 11 ]. In recent years, the contents of total polysaccharides, total saponins, and some medicinal components, such as total flavonoids, have primarily been used as criteria to evaluate the quality of germplasms and age sections of Polygonatum species.…”

“…A previous study of the genus Polygonatum revealed that adenosine, sucrose, and pyroglutamic acid could be used as markers to distinguish three Polygonatum species ( Polygonatum kingianum Coll. et Hemsl, Polygonatum cyrtonema Hua, and Polygonatum sibiricum Red) by ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) metabolomics [ 9 ]. In addition, the nutritional and medicinal components of different types of rhizomes of P. cyrtonema and Polygonatum sibiricum Red have been explored based on high-performance liquid chromatography coupled with charged aerosol detection (HPLC–CAD) and ultrahigh-performance liquid chromatography-Orbitrap-tandem mass spectrometry (UHPLC-Orbitrap-MS/MS) metabolomics [ 10 ].…”

Comparison of Semen Aesculi species from different geographical origins and horse chestnut based on metabolomics and chemometrics analysis

“…Metabolomics is a method for the comprehensive qualitative and quantitative examination of all metabolites in a biological system under predetermined conditions, 18 which can be used to analyse all small molecular metabolites in plants with different species, genotypes, and ecological types and to identify differences between samples 19 . At present, plant metabolomic is mainly applied for the quality control links of TCMs, such as source identification, 20 authenticity identification, 21 and processing method evaluation 22 . In addition, plant metabolomics is a well‐established research field in the large‐scale phytochemical analysis of TCMs, which is utilised for the qualitative and quantitative analysis of all ingredients derived from multiple plants 23 .…”