Metabolomics Study on Improvement of Mild Cognitive Impairment by SCPE: a  UPLC-Q/TOF-MS Mass Spectrometric Study

Abstract

Background: Mild Cognitive Impairment (MCI) is a common and easilyneglected neurological disease in clinic. Aging is the main cause of the disease,but its exact pathogenesis is not yet clear. Sagacious Confucius' PillowElixir(SCPE) is a classic medicine for the treatment of amnesia in traditionalChinese medicine.The aim of this study was to prove the stability and reliabilityof SAMP8 mice as MCI model, and to nd potential biomarkers for thetransformation of mild cognitive impairment to dementia, so as to reveal thepathogenesis of mild cognitive impairment and the mechanism of SCPE at thewhole animal level.Methods: Senescence-accelerated P8 (SAMP8) mice were selected as the modelof MCI. Based on UHPLC/Q-TOF-MS platform, the eect of SCPE on MCI micewas evaluated by metabolomics to screen and identify brain tissue biomarkers andpathways of MCI. T test of SPSS 22.0 software and meta-analysis software wereused for univariate and multivariate analysis to screen and identify the biomarkersand pathways of brain tissue with mild cognitive impairment; SPSS 22.0statistical software was used to conduct t-test on the total ionic strengthcorresponding to the dierent metabolites in each group, and to clarify thechange mechanism of biomarkers and pathways in dierent doses of SCPE.Results: From the point of view of brain metabolomics, 84 MCI-relatedbiomarkers and 8 metabolic pathways were identied. It was found that dierentdoses of SCPE could call back 36 same metabolic markers and 3 commonmetabolic pathways.And with the increase of dose, the number of SCPE callbackmetabolic markers gradually increased(LD:40; MD:48; HD:54), and the types ofmain metabolic pathways also changed to a certain extent (the biosynthesis ofvaline, leucine and isoleucine in low dose changed to cysteine and methioninemetabolic pathway in middle and high dose),which may be related to the eectof aminoacyl tRNA.Conclusions: The stability and reliability of MCI model, and the eectiveness ofSCPE in the treatment of MCI were clared. Compared three dierent dosegroups, the high dose SCPE group has more advantages.

Integrated Metabolomics and Transcriptomics Analysis Identifies Molecular Subtypes within the Early and Late Mild Cognitive Impairment Stages of Alzheimer’s Disease

“…Both EMCI-2 and LMCI-2 also show higher activation of the aminoacyl-tRNA biosynthesis. However, the activity of Aminoacyl-tRNA as it relates to AD disease progression is complex and not well understood (Kron, 2013), although numerous studies have mentioned this pathway in their results (Trushina, 2013;Wang, 2020;Hou, 2020). A suppressed zinc ion binding pathway, on the other hand, has been linked to oxidative stress by means of lipid peroxidation, an increase in osmotic fragility of erythrocyte membranes, and a decrease in protein turnover (Jurowski, 2014;Roohani, 2013).…”

Electroacupuncture alleviates the symptom of depression in mice by regulating the cGAS-STING-NLRP3 signaling

“…Furthermore, the NLRP3 and downstream inflammatory signaling pathways can be activated by the CGAS-STING signaling [20,21]. It is reported that aging-related cerebral infarction is alleviated by EA through downregulating the NLRP3/Caspase-1 signaling [22]. Currently, the regulatory relationship between CGAS-STing-NLRP3 signaling axis and depression has not been clarified.…”