Metabolomics Study on the Resistance of Walnut Peel to Colletotrichum gloeosporioides under Prochloraz Treatment

Abstract

Anthracnose, caused by Colletotrichum gloeosporioides, is highly harmful for walnut production in the world. To better control this disease, the inhibitory effects of 3 fungicides against Colletotrichum gloeosporioides were determined, and the results showed that prochloraz had better inhibitory activity. Through comparative metabolomics analysis, 311 metabolites might be associated with the walnut peel response to Colletotrichum gloeosporioides under prochloraz treatment. Furthermore, we supposed that the phenylpropanoid pathway might be induced by prochloraz to resist Colletotrichum gloeosporioides infection. In conclusion, the upregulated metabolites in the phenylpropanoid pathway might be related to synthesize lignin to further form a cell wall against Colletotrichum gloeosporioides infection.

Identification of the Ilex macrocarpa anthracnose pathogen and the antifungal potential of the cell-free supernatant of Bacillus velezensis against Colletotrichum fioriniae

“…A low concentration (0.1 mg/L) of prochloraz achieved a 50% inhibition rate, indicating its effectiveness at this concentration. Prochloraz functions as a broad-spectrum fungicide by inhibiting sterol biosynthesis and interfering with cell wall biosynthesis ( Zhang et al, 2021 ; Yang et al, 2022 ), which was confirmed in our study.…”

Table_1.DOCX

“…A low concentration (0.1 mg/L) of prochloraz achieved a 50% inhibition rate, indicating its effectiveness at this concentration. Prochloraz functions as a broad-spectrum fungicide by inhibiting sterol biosynthesis and interfering with cell wall biosynthesis (Zhang et al, 2021;Yang et al, 2022), which was confirmed in our study. However, biological control is preferred because it is environmentally friendly and less likely to cause mutations in pathogens.…”