Metabonomics Analysis of Stem Extracts from Dalbergia sissoo

Abstract

Dalbergia sissoo is a woody plant with economic and medicinal value. As the pharmacological qualities and properties of the wood from this plant primarily depend on its extractives, in this study, the metabolomic analysis of extractives from its stems was carried out using UPLC-MS/MS. A total of 735 metabolites were detected from two groups of samples, heartwood and sapwood, with the largest number of terpenoids in type and the largest number of flavonoids in quantity. The PCA and cluster analysis showed significant differences in the metabolite composition between the two groups. The differential metabolites were mainly organic oxygen compounds, flavonoids, and isoflavones. Among the 105 differential metabolites, 26 metabolites were significantly higher in relative content in sapwood than in heartwood, while the other 79 metabolites were significantly higher in relative content in heartwood than in sapwood. KEGG metabolic pathway enrichment analysis showed that these differential metabolites were mainly enriched in three metabolic pathways: Flavonoid biosynthesis, isoflavonoid biosynthesis, and flavonoid and flavonol biosynthesis. This study provides a reference for metabolomics studies in Dalbergia and other woody plants.

Chromosome-scale genome of Indian rosewood (Dalbergia sissoo)

“…Dalbergia sissoo , commonly known as Indian rosewood, is an ecologically and economically vital tropical timber species ( Bhagwat et al., 2015 ). However, a lack of genomic resources has constrained genetic research in D. sissoo , including studies of heartwood formation, a key trait for timber quality ( Hong et al., 2020 ; Hung et al., 2020 ) ( Li et al., 2022 ). The availability of high-quality chromosome-level reference genomes can greatly advance genetic research in economically valuable plant species ( Sahu and Liu, 2023 ).…”

“…Most of these heartwood components are extracts or secretions produced from metabolism and degradation of certain tissues. They give the heartwood a unique appearance and exhibit resistance to pathogenic fungi as potential biological protectants ( Li et al., 2022 ). However, there is little overall understanding of the molecular basis of D. sissoo secondary metabolite biosynthesis pathways, which hinders progress in molecular breeding and revealing its heartwood formation mechanism.…”

Integration of High-Resolution LC-Q-TOF Mass Spectrometry and Multidimensional Chemical−Biological Analysis to Detect Nanomolar-Level Acetylcholinesterase Inhibitors from Different Parts of Zanthoxylum nitidum

“…Rafi et al identified 31 compounds from Andrographis paniculata by LC-Orbitrap-MS/MS, and classified leaves and stems on the basis of 18 components by PCA . Li et al discovered that the differential compounds between heartwood and sapwood of Dalbergia sissoo were mainly organic oxygen compounds, flavonoids, and isoflavones by LC-QTOF-MS, PCA, and cluster analysis . To further discover active components in plants, virtual screening such as molecular docking has gradually emerged due to its advantages of low cost and short time.…”