Metabonomics Approach To Determine Metabolic Differences between Green Tea and Black Tea Consumption

Abstract

The purpose of this study was to compare the effects of black and green tea consumption on human metabolism. Seventeen healthy male volunteers consumed black tea, green tea, or caffeine in a randomized crossover study. Twenty-four-hour urine and blood plasma samples were analyzed by NMR-based metabonomics, that is, high-resolution 1H NMR metabolic profiling combined with multivariate statistics. Green and black tea consumption resulted in similar increases in urinary excretion of hippuric acid and 1,3-dihydroxyphenyl-2-O-sulfate, both of which are end products of tea flavonoid degradation by colonic bacteria. Several unidentified aromatic metabolites were detected in urine specifically after green tea intake. Interestingly, green and black tea intake also had a different impact on endogenous metabolites in urine and plasma. Green tea intake caused a stronger increase in urinary excretion of several citric acid cycle intermediates, which suggests an effect of green tea flavanols on human oxidative energy metabolism and/or biosynthetic pathways.

Metabolomic Pattern Analysis after Mediterranean Diet Intervention in a Nondiabetic Population: A 1- and 3-Year Follow-up in the PREDIMED Study

“…Traditionally, studies centred on diet have focused on a single nutrient or dietary component in the evaluation of dietary intake (1,2). However, the assessment of dietary patterns to elucidate the beneficial effects in complex disease processes is increasing.…”

Questionnaire-based self-reported nutrition habits associate with serum metabolism as revealed by quantitative targeted metabolomics

“…Examples would be the effect of meat and fish consumption on the risk of colorectal cancer, metabolic differences in the intake of green and black tea or the metabolic effects of dietary isoflavones in humans [4][5][6][7][8][9][10][11]. However, directly associating genes or lifestyle habits with clinical endpoints provides only limited information about the underlying disease-causing mechanisms.…”

Assessment of dietary exposure related to dietary GI and fibre intake in a nutritional metabolomic study of human urine

“…Hippurate has also been reported as a metabolic end product of plant phenols and flavonoids by the gut microflora (Daykin et al 2005;Mulder et al 2005;Nicholson et al 2005;Van Dorsten et al 2006;Wang et al 2005) and hence, it can also be speculated that hippurate is partially a metabolic signature by the gut microflora of the subjects participating in the present study.…”