Metabonomics, dietary influences and cultural differences: a 1H NMR-based study of urine samples obtained from healthy British and Swedish subjects

Lenz, Eva M.; Bright, Jonathan; Wilson, Ian D.; Hughes, Andrew; Morrisson, J.; Lindberg, Henrik; Lockton, A.

doi:10.1016/j.jpba.2004.08.002

Cited by 246 publications

(201 citation statements)

References 26 publications

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“…The study of urine samples obtained from healthy British and Swedish subjects revealed characteristic dietary and cultural features between the subjects of both countries, such as high trimethylamine-N-oxide (TMAO)-excretion in the Swedish population and high taurine-excretion, due to the Atkins diet [33]. Urine samples have also been used to investigate responses to ingestion of chamomile (Matricaria recutita) tea.…”

Section: Metabolomics Approaches Applied To Measurement Of Dietary Exmentioning

confidence: 99%

Measurement of dietary exposure: a challenging problem which may be overcome thanks to metabolomics?

et al. 2009

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The diet is an important environmental exposure, and its measurement is an essential component of much health-related research. However, conventional tools for measuring dietary exposure have significant limitations being subject to an unknown degree of misreporting and dependent upon food composition tables to allow estimation of intakes of energy, nutrients and non-nutrient food constituents. In addition, such tools may be inappropriate for use with certain groups of people. As an alternative approach, the recent techniques of metabolite profiling or fingerprinting, which allows simultaneous monitoring of multiple and dynamic components of biological fluids, may provide metabolic signals indicative of food intake. Samples can be analysed through numerous analytical platforms, followed by multivariate data analysis. In humans, metabolomics has been applied successfully in pharmacology, toxicology and medical screening, but nutritional metabolomics is still in its infancy. Biomarkers of a small number of specific foods and nutrients have been developed successfully but less targeted and more high-throughput methods, that do not need prior knowledge of which signals might be discriminatory, and which may allow a more global characterisation of dietary intake, remain to be tested. A proof a principle project (the MEDE Study) is currently underway in our laboratories to test the hypothesis that high-throughput, non-targeted metabolite fingerprinting using flow injection electrospray mass spectrometry can be applied to human biofluids (blood and urine) to characterise dietary exposure in humans.

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Section: Metabolomics Approaches Applied To Measurement Of Dietary Exmentioning

confidence: 99%

Measurement of dietary exposure: a challenging problem which may be overcome thanks to metabolomics?

et al. 2009

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“…However, the anthropometric data is often sufficient: height, body weight, body mass index (weight ratio in kg/height in m high per square), fat mass percentages and lean mass (measured by impedance measurement). Table 1 shows the main anthropometric and bioumoral parameters used in the general evaluation of nutrition status [40][41][42][43][44][45][46][47][48][49][50][51][52][53].…”

Section: Molecular Diagnosis Of the State Of Nutritionmentioning

confidence: 99%

“…A classic nutritional deficiency index is hypoalbuminemia, particularly in elderly subjects. Other laboratory indices useful in this regard are quantization of transferrin and lymphocytes in the blood [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57] (Table 2).…”

Section: Molecular Diagnosis Of the State Of Nutritionmentioning

confidence: 99%

Nutrition and nutrigenomics: an overview

Capasso¹,

Tecce²

2017

Integr Food Nutr Metab

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“…Overall, the general feeling is that urine reflects a more short-term state of the organism, whereas fasting serum and/or plasma changes represent more chronic or long-term snapshots of the system. There is also the influence of ethnicity, diet, diurnal rhythm, and so on, on the experimental metabolome to consider [62,63].…”

Section: Sizes Of the Human And Other Metabolomesmentioning

confidence: 99%

Systems biology, metabolic modelling and metabolomics in drug discovery and development

Kell

2006

Drug Discovery Today

266

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Unlike signalling pathways, metabolic networks are subject to strict stoichiometric constraints. Metabolomics amplifies changes in the proteome, and represents more closely the phenotype of an organism. Recent advances enable the production (and computer-readable encoding as SBML) of metabolic network models reconstructed from genome sequences, as well as experimental measurements of much of the metabolome. There is increasing convergence between the number of human metabolites estimated via genomics ( 3000) and the number measured experimentally. It is thus both timely, and now possible, to bring these two approaches together as an integrated (if distributed) whole to help understand the genesis of metabolic biomarkers, the progress of disease, and the modes of action, efficacy, off-target effects and toxicity of pharmaceutical drugs. Systems biology and metabolic modelling in the 21st CenturyAlthough there are many individual definitions, most commentators (including this one [1,2]) take it that systems biology involves an iterative interplay between more or less high-throughput and high-content 'wet' experiments, technology development, theory and computational modelling, and that it is the involvement of computational modelling, in particular, in the process that sets systems biology apart from the more traditional and more reductionist molecular biology. Metabolomics illustrates this amply. There is also the view that the perceived decrease in the effectiveness of the target-based drug discovery process [3][4][5], including the still-high levels of attrition [6], means that we must move towards understanding organisms at something more akin to a whole-system level [7][8][9][10][11].The question then arises as to what part of a system one might first beneficially model? Although there has, unsurprisingly, been considerable interest in modelling the major signalling pathways (e.g. Refs [12,13]), there are several reasons why it is timely to turn our attention to the level of small molecule metabolism, which is the focus of this review. Metabolism is more discriminatingIt has long been known, and proven through the formalism of metabolic control analysis [7,10,[14][15][16], that whereas small changes in the concentrations of enzymes (and the transcripts that encode them) have only small effects on the fluxes through metabolic pathways, they have substantial effects on the concentrations of metabolic intermediates. Because the metabolome (nominally the concentrations of 'all' the metabolites measured in a system of interest [17]) is downstream of the proteome, it is thereby 'amplified' both in theory [18] and in practice [19,20] and represents a more sensitive level of organisation than do the macromolecular 'omes for understanding a complex biological system, and the changes in it that might be occasioned by disease or pharmaceutical intervention [21,22]. Metabolic reconstruction is now mature and timelyAn attractive feature for the purposes of modelling is that metabolism, in contrast to signalling pathways, ...

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Metabonomics, dietary influences and cultural differences: a 1H NMR-based study of urine samples obtained from healthy British and Swedish subjects

Cited by 246 publications

References 26 publications

Measurement of dietary exposure: a challenging problem which may be overcome thanks to metabolomics?

Measurement of dietary exposure: a challenging problem which may be overcome thanks to metabolomics?

Nutrition and nutrigenomics: an overview

Systems biology, metabolic modelling and metabolomics in drug discovery and development

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