Metabotropic Glutamate Receptors

Dillon, James; Franks, Christopher J.; Murray, Caitriona; Edwards, Richard J.; Calahorro, Fernando; Ishihara, Takeshi; Izui, Katsura; Holden‐Dye, Lindy; O’Connor, Vincent

doi:10.1074/jbc.m114.606608

Cited by 31 publications

(22 citation statements)

References 44 publications

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“…There is also evidence that the metabotropic glutamate receptor, MGL-1, plays a role in feeding behaviour following acute removal of food in C. elegans (Dillon et al 2015). Following acute removal of food, mgl-1 mutants pump at a raised rate compared to N2, indicating that activation of MGL-1 (which is widely expressed in the extrapharyngeal and pharyngeal nervous system including NSM) leads to inhibition of pharyngeal pumping.…”

Section: Glutamate and Pharyngealmentioning

confidence: 99%

Caenorhabditis elegans Feeding Behaviors

Dallière¹,

Holden‐Dye²,

Dillon³

et al. 2017

Oxford Research Encyclopedia of Neuroscience

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The microscopic free-living nematode worm Caenorhabditis elegans was the first metazoan to have its genome sequenced and for many decades has served as a genetically tractable model for the investigation of neural mechanisms of behavioral plasticity. Many of its behaviors involve the detection of its food, bacteria, which are ingested and transported to the intestine by a muscular pharynx. The structure of the pharynx and the circuitry of the pharyngeal nervous system that regulates pharyngeal activity have been described in some detail. This has provided a platform for understanding how this simple organism finely tunes its feeding behavior in response to the changing availability and quality of its food, and in the context of its own nutritional status. This resonates with fundamental principles of energy homeostasis that occur throughout the animal kingdom.

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Section: Glutamate and Pharyngealmentioning

confidence: 99%

Caenorhabditis elegans Feeding Behaviors

Dallière¹,

Holden‐Dye²,

Dillon³

et al. 2017

Oxford Research Encyclopedia of Neuroscience

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“…These results showed how degenerate neural pathways, in which multiple elements can perform the same function, regulate behaviour in a simple model system. Dillon et al [96] used ChR2-mediated excitation of glutamatergic pharyngeal neurons to investigate the function of metabotropic glutamate receptors (mGluRs). The authors found that glutamate receptors are required for presynaptic modulation of pharyngeal behaviour and electrical activity.…”

Section: Pharyngeal Neuronsmentioning

confidence: 99%

Illuminating neural circuits and behaviour in Caenorhabditis elegans with optogenetics

Fang-Yen

Alkema

Samuel

2015

Phil. Trans. R. Soc. B

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The development of optogenetics, a family of methods for using light to control neural activity via light-sensitive proteins, has provided a powerful new set of tools for neurobiology. These techniques have been particularly fruitful for dissecting neural circuits and behaviour in the compact and transparent roundworm Caenorhabditis elegans. Researchers have used optogenetic reagents to manipulate numerous excitable cell types in the worm, from sensory neurons, to interneurons, to motor neurons and muscles. Here, we show how optogenetics applied to this transparent roundworm has contributed to our understanding of neural circuits.

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“…The Caenorhabditis elegans genome encodes three metabotropic glutamate receptors, named MGL-1, MGL-2 and MGL-3. Sequence analysis suggests the three receptors each representing orthologues of one of the mammalian mGluR subfamilies, and the functional diversity within the mammalian family is conserved in C. elegans (Dillon et al 2015). There is currently a limited understanding of this class of receptor, compared to the ionotropic families of glutamate receptors (viz NMDA, AMPA and Ivermectin channels) in C. elegans.…”

Section: Introductionmentioning

confidence: 99%

“…We have probed its function using the mGluR agonist L-CCG-I to show that it is a negative regulator of pharyngeal network activity. MGL-1 is required for coordinating plasticity in feeding behaviour and reproduction upon the removal of food (Dillon et al 2015;Jeong and Paik 2017). To build upon this, we have identified candidate MGL scaffolding proteins and screened for their potential to modulate the activity of the pharyngeal network.…”

Section: Introductionmentioning

confidence: 99%

Yeast two-hybrid screening identifies MPZ-1 and PTP-1 as candidate scaffolding proteins of metabotropic glutamate receptors in Caenorhabditis elegans

2018

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The metabotropic glutamate receptors (mGluRs) are a class of G-protein-coupled receptor that undergo extensive interactions with scaffolding proteins, and this is intrinsic to their function as an important group of neuromodulators at glutamatergic synapses. The Caenorhabditis elegans nervous system expresses three metabotropic glutamate receptors, MGL-1, MGL-2 and MGL-3. Relatively little is known about how the function and signalling of these receptors is organised in C. elegans. To identify proteins that scaffold the MGL-1 receptor, we have conducted a yeast two-hybrid screen. Three of the interacting proteins, MPZ-1, NRFL-1 and PTP-1, displayed motifs characteristic of mammalian mGluR scaffolding proteins. Using cellular co-expression criterion, we show mpz-1 and ptp-1 exhibited overlapping expression patterns with subsets of mgl-1 neurons. This included neurones in the pharyngeal nervous system that control the feeding organ of the worm. The mGluR agonist L-CCG-I inhibits the activity of this network in wild-type worms, in an MGL-1 and dose-dependent manner. We utilised L-CCG-I to identify if MGL-1 function was disrupted in mutants with deletions in the mpz-1 gene. The mpz-1 mutants displayed a largely wild-type response to L-CCG-I, suggesting MGL-1 signalling is not overtly disrupted consistent with a non-obligatory modulatory function in receptor scaffolding. The selectivity of the protein interactions and overlapping expression identified here warrant further investigation of the functional significance of scaffolding of metabotropic glutamate receptor function.

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Metabotropic Glutamate Receptors

Cited by 31 publications

References 44 publications

Caenorhabditis elegans Feeding Behaviors

Caenorhabditis elegans Feeding Behaviors

Illuminating neural circuits and behaviour in Caenorhabditis elegans with optogenetics

Yeast two-hybrid screening identifies MPZ-1 and PTP-1 as candidate scaffolding proteins of metabotropic glutamate receptors in Caenorhabditis elegans

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