Metachromatic Agar-Diffusion Microslide Technique for Detecting Staphylococcal Nuclease in Foods

Lachica, R. V. F.; Hoeprich, P. D.; Genigeorgis, C.

doi:10.1128/aem.23.1.168-169.1972

Cited by 27 publications

(18 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, the use of ethidium bromide rather than toluidine blue to detect nuclease activity has been advocated by Kulkarni & Barve (1983). For detection of TNase in food, the food is either assayed directly (Lachica et al 1972) or some form of extraction is carried out before assay (Tatini et al 1976;Ibrahim 1981;McClain et al 1984).…”

Section: Thermonucleasementioning

confidence: 99%

Staphylococci in milk and milk products

Gilmour¹,

Harvey²

1990

Journal of Applied Bacteriology

View full text Add to dashboard Cite

Section: Thermonucleasementioning

confidence: 99%

Staphylococci in milk and milk products

Gilmour¹,

Harvey²

1990

Journal of Applied Bacteriology

View full text Add to dashboard Cite

“…These include human and bovine strains, with strain JA-1 being a natural isolate from cheese. (4) The sensitivity of the thermonuclease assay is on the same order as other methods reported (Cordsand Tatini 1973;Lachica et al 1972;Tatini et al 1976). The utilization of a simple 2 h extraction procedure allows detection of samples representing five g of food containing only 250 ng of thermonuclease.…”

Section: Detection Of Staphylococcus Aureus Products In Foods 23mentioning

confidence: 52%

DETECTION OF STAPHYLOCOCCUS AUREUS PRODUCTS IN FOODS USING ENZYME LINKED IMMUNOSORBENT ASSAY AND SPECTROPHOTOMETRIC THERMONUCLEASE ASSAY

Man

Chesbro

1980

Journal of Food Safety

View full text Add to dashboard Cite

The comparative sensitivity of an enzyme linked immunosorbent assay (ELISA) using four different antistaphylococcal antisera and a spectrophotometric assay for thermonuclease were determined using cheese and ravioli samples seeded with strains of Staphylococcus aureus and S. epidermidis. The ELISA used antisera to enterotoxin A, enterotoxin B, S. aureus strains 14609 (human), and UNH‐570 (bovine). The 570 ELISA and spectrophotometric thermonuclease assay were of comparable sensitivity and detected seeded culture in concentrations as low as 2 × 107 CFU/g of cheese. A simple two hour method for extracting thermonuclease from foods was 50% efficient when as little as 50 ng of purified enzyme was seeded per g of cheese. Analyses of 43 commercial cheeses for viable S. aureus found five (12%) positive with 3 × 104 CFU/g of cheese being the highest counts detected. All samples were negative by ELISA and thermonuclease assay. A simple screening procedure for demonstration of S. aureus contamination of foods is discussed.

show abstract

“…The usefulness of the DNase test in screening foods for likely confirmation of enterotoxins presence has been demonstrated. Cords & Tatini (1973), Lachica et al . (1972) and Hirooka et al .…”

Section: Resultsmentioning

confidence: 98%

“…The DNase assay previously described by Lachica et al . (1972) was used according to the reference method given in IDF Standard 83 (1992).…”

Section: Methodsmentioning

confidence: 99%

An automated method for the detection of staphylococcal heat stable deoxyribonuclease in dairy products

Meyrand

Atrache

Bavai

et al. 1999

Lett Appl Microbiol

View full text Add to dashboard Cite

1999. An automated sandwich immunoassay with specific polyclonal antibodies for the detection of Staphylococcus aureus thermostable nuclease (DNase) is described. To evaluate this assay, different quantities of purified S. aureus nuclease were added to dairy products. Additionally, staphylococcal counts and nuclease activity of milk samples inoculated with S. aureus were determined. Different extraction procedures were performed and compared. The results indicated that the automated test was a reliable method for detecting DNase activity in milk products. The procedure was completed in 2 h and detected 1 ng of DNase ml −1 . Detection of the DNase was especially useful in cheeses and could be used to confirm positive enterotoxin results.

show abstract

Metachromatic Agar-Diffusion Microslide Technique for Detecting Staphylococcal Nuclease in Foods

Abstract: The metachromatic agar-diffusion (MAD) microslide technique was shown to detect nanogram quantities of staphylococcal thermonuclease in various foods without prior extraction, purification, or concentration.

Cited by 27 publications

References 3 publications

Staphylococci in milk and milk products

Staphylococci in milk and milk products

DETECTION OF STAPHYLOCOCCUS AUREUS PRODUCTS IN FOODS USING ENZYME LINKED IMMUNOSORBENT ASSAY AND SPECTROPHOTOMETRIC THERMONUCLEASE ASSAY

An automated method for the detection of staphylococcal heat stable deoxyribonuclease in dairy products

Contact Info

Product

Resources

About