Metadichol® Induced High Levels of Vitamin C: Case Studies

Pr, Raghavan

doi:10.4172/2376-1318.1000169

Cited by 9 publications

(2 citation statements)

References 21 publications

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“…Metadichol® is a nanoformulation of long-chain alcohols that in Zucker diabetic rats and humans has been shown to increases Vitamin C levels above and beyond what is achieved orally [1][2][3]. Vitamin C is a potent antioxidant required for many biological functions [4].…”

Section: Introductionmentioning

confidence: 99%

Metadichol®, Vitamin C and GULO Gene Expression in Mouse Adipocytes

Raghavan¹

2017

Biol Med

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Metadichol, a novel nano emulsion of lipid alcohols, up regulates the expression of GULO gene in mouse at picogram levels. 3T3-L1 pre adipocyte cells were differentiated using differentiating media. Post differentiation, the cells were treated with different concentrations of Metadichol for 24 hours and untreated cells served as control. Whole RNA was isolated after the treatment period and semi-quantitative reverse transcription PCR was run with GULO gene specific primer to obtain cDNA. Relative gene expression of GULO was determined by analysis of GULO gene amplicons using image J software. The GULO gene expression in the treated cells was up regulated fourfold relative to basal level of untreated cells.. Relative gene expression at concentrations 1 μg/ml, 100 ng/ml, 1 ng/ml, 100 pg/ml, 1 pg/ml was found to be 2.11, 2.64, 2.96, 3.96, 3.25 fold compared to control.

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Section: Introductionmentioning

confidence: 99%

Metadichol®, Vitamin C and GULO Gene Expression in Mouse Adipocytes

Raghavan¹

2017

Biol Med

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“…This induction of the expression of both insulin and __ glucagon in cancer cells might be explored as a novel therapeutic strategy because Metadichol ® is a nontoxic molecule and safe for human use. [62][63][64]…”

Section: Therapeutic Strategymentioning

confidence: 99%

Metadichol-Induced Differentiation of Pancreatic Ductal Cells (PANC-1) into Insulin-Producing Cells

Raghavan

2023

MRAJ

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Endocrine gene expression in PANC-1, a type of pancreatic cancer cell, has been studied in the context of their potential to be reprogrammed toward a normal, differentiated state. Alkaline phosphatase activity has also been shown in pluripotent stem cells to differentiate between feeder and parental cells in reprogramming experiments. Metadichol®-based cell programming holds promise as a versatile and potentially safer approach for manipulating cellular behavior without the use of viral vectors, gating, or CRISPR. Using qRT‒PCR the results show multifold increase in the gene expression of CA9, GCG, INS MAFA, NEUROD1, NGN3, NKX2-2, PAX6: PDX1, SLC2A2, FOXO1, and SIRT1. ALP levels increased and this activity is often used to distinguish stem cells from feeder cells as well as from parental cells in reprogramming experiments. Pluripotency was confirmed by the presence of islet-like structures on day eight. Metadichol exhibits anticancer activity with a CC50 value of 5.50 µg/ml compared to standard doxorubicin with a CC50 value of 10.28 µg/ml. At 100 ug/ml Metadichol is 82% cytotoxic.in a MTT assay Anti-tumor gene Klotho’s expression was increased 70fold on day eight. All the genes seen expressed regulate endocrine cell development in the pancreas and are involved in insulin and glucagon secretion. Gene network analysis is presented to show how Metadichol induced expression leads to a closed loop feedback network and biological process that would help in mitigating diabetes and other related disorders.

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Metadichol® induced expression of neuronal transcription factors with Human fibroblast Dermal cells

Raghavan

2022

Preprint

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Background. Producing neurons from fibroblast cells has the potential to treat neurodegenerative diseases, characterized by neuron loss. Neurodegenerative diseases are a growing problem in the current aging, developed world populations. Metadichol® is a nontoxic nanoemulsion of long-chain lipid alcohols, currently available as an oral supplement. Methods and Findings. In this study, Metadichol® was used to treat human fibroblasts in vitro; we subsequently evaluated changes in the expression of neuronal transcription factors by qRT-PCR and immunoblotting. We observed increased expression of critical transcription factors for neuronal development, such as ASCL1, NGN2. ND2, NR4A2, LMX1A, LHX3. ISL1, and FOXA2. Conclusions. These data suggest that Metadichol® is a promising putative neuronal remodeling agent. Its current availability and safety profile suggest that it could be rapidly available for in vivo testing, impossible thus far.

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Metadichol® Induced High Levels of Vitamin C: Case Studies

Cited by 9 publications

References 21 publications

Metadichol®, Vitamin C and GULO Gene Expression in Mouse Adipocytes

Metadichol®, Vitamin C and GULO Gene Expression in Mouse Adipocytes

Metadichol-Induced Differentiation of Pancreatic Ductal Cells (PANC-1) into Insulin-Producing Cells

Metadichol® induced expression of neuronal transcription factors with Human fibroblast Dermal cells

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