Metagenome-Assembled Genome Sequence of Rhodopseudomonas palustris Strain ELI 1980, Commercialized as a Biostimulant

Crovadore, Julien; Xu, Shoutao; Chablais, Romain; Cochard, Bastien; Lukito, Delvia; Calmin, Gautier; Lefort, François

doi:10.1128/genomea.00221-17

Cited by 10 publications

(7 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Common traits found in sequenced B. polymyxa strains with plant-growth-promoting ability are phytohormone production and increased nutrient availability [ 90 ]. In the genome of the biofertilizer Rhodopseudomonas palustris ELI 1980, genes for antibiotic production, auxin biosynthesis, and nitrogen fixation were identified [ 91 ]. Newly reported Sphingomonas sp.…”

Section: How To Identify New Potent Microbial Traitsmentioning

confidence: 99%

Biofertilizers and Biocontrol Agents for Agriculture: How to Identify and Develop New Potent Microbial Strains and Traits

et al. 2021

View full text Add to dashboard Cite

Microbiological tools, biofertilizers, and biocontrol agents, which are bacteria and fungi capable of providing beneficial outcomes in crop plant growth and health, have been developed for several decades. Currently we have a selection of strains available as products for agriculture, predominantly based on plant-growth-promoting rhizobacteria (PGPR), soil, epiphytic, and mycorrhizal fungi, each having specific challenges in their production and use, with the main one being inconsistency of field performance. With the growing global concern about pollution, greenhouse gas accumulation, and increased need for plant-based foods, the demand for biofertilizers and biocontrol agents is expected to grow. What are the prospects of finding solutions to the challenges on existing tools? The inconsistent field performance could be overcome by using combinations of several different types of microbial strains, consisting various members of the full plant microbiome. However, a thorough understanding of each microbiological tool, microbial communities, and their mechanisms of action must precede the product development. In this review, we offer a brief overview of the available tools and consider various techniques and approaches that can produce information on new beneficial traits in biofertilizer and biocontrol strains. We also discuss innovative ideas on how and where to identify efficient new members for the biofertilizer and biocontrol strain family.

show abstract

Section: How To Identify New Potent Microbial Traitsmentioning

confidence: 99%

Biofertilizers and Biocontrol Agents for Agriculture: How to Identify and Develop New Potent Microbial Strains and Traits

et al. 2021

View full text Add to dashboard Cite

show abstract

“…We assayed SAGE in the phylogenetically distant alphaproteobacterium, Rhodopseudomonas palustris 60 . Able to grow under both anaerobic and aerobic conditions in heterotrophic, phototrophic, and chemolithotrophic modes of growth, R. palustris has one of the most versatile metabolic repertoires known bacteria, and are also often PGPR [61][62][63][64] .…”

Section: Sage Enables Efficient Genome Engineering In Diverse Bacteriamentioning

confidence: 99%

The SAGE genetic toolkit enables highly efficient, iterative site-specific genome engineering in bacteria

Elmore

Francis

et al. 2020

Preprint

View full text Add to dashboard Cite

Sustainable enhancements to crop productivity and increased resilience to adverse conditions are critical for modern agriculture, and application of plant growth promoting rhizobacteria (PGPR) is a promising method to achieve these goals. However, many desirable PGPR traits are highly regulated in their native microbe, limited to certain plant rhizospheres, or insufficiently active for agricultural purposes. Synthetic biology can address these limitations, but its application is limited by availability of appropriate tools for sophisticated, high-throughput genome engineering that function in environments where selection for DNA maintenance is impractical. Here we present an orthogonal, Serine-integrase Assisted Genome Engineering (SAGE) system, which enables iterative, site-specific integration of up to 10 different DNA constructs at efficiency on par or better than replicating plasmids. SAGE does not require use of replicating plasmids to deliver recombination machinery, and employs a secondary serineintegrase to excise and recycle selection markers. Furthermore, unlike the widely utilized pBBR1 origin, DNA transformed using SAGE is stable without selection. We highlight SAGE's utility by constructing a 287-member constitutive promoter library with a ~40,000-fold dynamic range in P. fluorescens SBW25. We show that SAGE functions robustly in diverse ⍺and "-proteobacteria, thus providing evidence that it will be broadly useful for engineering industrial or environmental bacteria.

show abstract

“…Since then, it has been isolated from a wide variety of environments, including aquatic sediments ( 2 ), sludge ( 3 , 4 ), soils ( 5 ), alkaline waters ( 6 ), and eutrophic ponds ( 7 ), and it can be cultivated under several conditions. Therefore, R. palustris has potential in many applications ranging from hydrogen and electricity production ( 4 , 8 – 10 ) to bioremediation of aromatic compounds, including the important environmental pollutant skatole, and the dehalogenation of carboxylic acids ( 11 – 13 ), and it has even been commercialized as a biostimulant for agriculture ( 14 ).…”

Section: Announcementmentioning

confidence: 99%

“…Several R. palustris strains have been sequenced since 2004 ( 13 – 15 ). The XCP strain was isolated in 1966 as a contaminant of a green bacterial culture in La Jolla, California, and labeled XCP for “ex Chlorobaculum parvum .” Its cytochrome C2 and C′ sequences were determined ( 16 , 17 ), which clearly indicated that this strain was different from any of the other known R. palustris cultures and called into question the amount of diversity allowed within a species.…”

Section: Announcementmentioning

confidence: 99%

Draft Whole-Genome Sequence of the Purple Photosynthetic Bacterium Rhodopseudomonas palustris XCP

Rayyan

Meyer

Kyndt

2018

Microbiol Resour Announc

View full text Add to dashboard Cite

show abstract

Metagenome-Assembled Genome Sequence of Rhodopseudomonas palustris Strain ELI 1980, Commercialized as a Biostimulant

Abstract: We report here the draft genome sequence of strain ELI 1980 of Rhodopseudomonas palustris, commercialized as a biostimulant for agriculture. The genome was reconstructed from the metagenome of a commercial product containing this strain as its major component.

Cited by 10 publications

References 30 publications

Biofertilizers and Biocontrol Agents for Agriculture: How to Identify and Develop New Potent Microbial Strains and Traits

Biofertilizers and Biocontrol Agents for Agriculture: How to Identify and Develop New Potent Microbial Strains and Traits

The SAGE genetic toolkit enables highly efficient, iterative site-specific genome engineering in bacteria

Draft Whole-Genome Sequence of the Purple Photosynthetic Bacterium Rhodopseudomonas palustris XCP

Contact Info

Product

Resources

About