Metagenome Assembly and Metagenome-Assembled Genome of “
            <i>Candidatus</i>
            Aramenus sulfurataquae” from Thermal Sediments from the Los Azufres Volcanic Complex

Marín-Paredes, Roberto; Tapia-Torres, Yunuen; Martı́nez-Romero, Esperanza; Quesada, Maurício; Servín-Garcidueñas, Luis E.

doi:10.1128/mra.00379-21

Cited by 4 publications

(4 citation statements)

References 15 publications

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“…in 2017 using high-quality metagenome-assembled genome analysis to describe the representative bacterium EM3 [ 100 ]. Since then, no members of this phylum have been cultivated, which is often the case in hot spring environments [ 101 , 102 ]. In our dataset, putative members of this phylum were detected mostly in sample V, forming up to 14% of reads.…”

Section: Discussionmentioning

confidence: 99%

Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis

Smrhova

Jani

Pajer

et al. 2022

Environmental Microbiome

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Background The extreme conditions of thermal springs constitute a unique aquatic habitat characterized by low nutrient contents and the absence of human impacts on the microbial community composition. Thus, these springs may host phylogenetically novel microorganisms with potential use in biotechnology. With this hypothesis in mind, we examined the microbial composition of four thermal springs of the world-renowned spa town of Karlovy Vary (Carlsbad), Czechia, which differ in their temperature and chemical composition. Results Microbial profiling using 16S rRNA gene sequencing revealed the presence of phylogenetically novel taxa at various taxonomic levels, spanning from genera to phyla. Many sequences belonged to novel classes within the phyla Hydrothermae, Altiarchaeota, Verrucomicrobia, and TA06. Cultivation-based methods employing oligotrophic media resulted in the isolation of 44 unique bacterial isolates. These include strains that withstand concentrations of up to 12% NaClw/v in cultivation media or survive a temperature of 100 °C, as well as hitherto uncultured bacterial species belonging to the genera Thermomonas, Paenibacillus, and Cellulomonas. These isolates harbored stress response genes that allow them to thrive in the extreme environment of thermal springs. Conclusions Our study is the first to analyze the overall microbial community composition of the renowned Karlovy Vary thermal springs. We provide insight into yet another level of uniqueness of these springs. In addition to their unique health benefits and cultural significance, we demonstrate that these springs harbor phylogenetically distinct microorganisms with unusual life strategies. Our findings open up avenues for future research with the promise of a deeper understanding of the metabolic potential of these microorganisms.

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Section: Discussionmentioning

confidence: 99%

Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis

Smrhova

Jani

Pajer

et al. 2022

Environmental Microbiome

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“…This low abundance may be due to the type of sample. Sulfolobales archea were found in water samples from an acid solfatara [15] and in a yellow sediment sample (sulfur crystal) [18]. Thirteen MAGs associated with Thermoplasmatales were discovered by us, and phylogenomic research reveals that these archaea are very diverse.…”

Section: Discussionmentioning

confidence: 95%

“…In addition, another genome of Ca. Aramenus [18] was obtained. In the last three years, two metagenomes have been sequenced from green sediments around a hot spring and around a fumarole.…”

Section: Introductionmentioning

confidence: 99%

A Metagenome from a Steam Vent in Los Azufres Geothermal Field Shows an Abundance of Thermoplasmatales archaea and Bacteria from the Phyla Actinomycetota and Pseudomonadota

Marín-Paredes,

Bolívar-Torres,

Coronel-Gaytán

et al. 2023

CIMB

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Los Azufres National Park is a geothermal field that has a wide number of thermal manifestations; nevertheless, the microbial communities in many of these environments remain unknown. In this study, a metagenome from a sediment sample from Los Azufres National Park was sequenced. In this metagenome, we found that the microbial diversity corresponds to bacteria (Actinomycetota, Pseudomonadota), archaea (Thermoplasmatales and Candidatus Micrarchaeota and Candidatus Parvarchaeota), eukarya (Cyanidiaceae), and viruses (Fussellovirus and Caudoviricetes). The functional annotation showed genes related to the carbon fixation pathway, sulfur metabolism, genes involved in heat and cold shock, and heavy-metal resistance. From the sediment, it was possible to recover two metagenome-assembled genomes from Ferrimicrobium and Cuniculiplasma. Our results showed that there are a large number of microorganisms in Los Azufres that deserve to be studied.

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“…Nostocaceae (Ansary et al, 2017), Brevibacillaceae (Inan et al, 2016), Paenibacillaceae (Panosyan et al, 2020), and Hydrothermae (Smrhova et al, 2022). Much affiliation remains vague at the order or even class level, since hot springs are extreme environments from which few microorganisms can be cultured and studied (López-López et al, 2013;Marín-Paredes et al, 2021); this justifies the need to multiply the studies of these extreme environments In order to better understand them. Our results illustrate variation in taxonomic diversity both spatially (i.e., between Site 4 2021 (zone 13), Site 4 2021 (zone 15), and Site 4 2021 (zone 16)) and, particularly, temporally (i.e., between 2019 and 2021).…”

Section: H 2 Productionmentioning

confidence: 99%

Natural hydrogen potential and basaltic alteration in the Asal–Ghoubbet rift, Republic of Djibouti

Pasquet¹,

Idriss²,

Ronjon-Magand³

et al. 2023

BSGF - Earth Sci. Bull.

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The Asal–Ghoubbet active rift in the Republic of Djibouti is a site of interest for geothermal energy and natural hydrogen, and previous studies have indicated that dihydrogen (H2) emanates from this rift. However, the well-known serpentinization reaction does not appear to be the main mechanism generating H2 at this site. Rather, the H2 is generated as follows: (1) by alteration of basaltic lava at depth via reaction with seawater flowing from Ghoubbet Bay towards Lake Asal; (2) by simple degassing of the volcanic chamber located a few kilometers below the Fiale Caldera in the rift axis; or (3) as a result of pyritization processes via the oxidation of H2S. Analysis of microorganisms did not indicate any production or consumption of H2, CO2, or CH4; therefore, it is unlikely that microorganisms affected H2 gas contents measured at the surface. However, air contamination at fumaroles is typically considerable and may limit interpretation of such processes. Drill cuttings from the Fiale 1 (F1) and Gale le Goma 1 (Glc1) wells (located on the inner and outer rift margins, respectively) were analyzed to determine where H2 is generated. Total rock analyses indicated distinct zones at depths of 464 m and 280 m for F1 and Glc1, respectively, representing the boundary between the Asal and Stratoïd Basalts. 57Fe Mössbauer analyses show a decrease in the percentage of Fe3+ at depth, indicating that Fe2+-rich material, particularly in the Stratoïd Basalts, may be a source of H2. Based on well data from the rift center and the outer rift margin, it is evident that H2 is present at the surface in the rift axis and that this area offers good remnant potential because of the presence of Fe-rich chlorite. Conversely, few H2 emissions were measured at the surface on the outer rift margins, although well data showed some H2 (~0.25%) at depth. The presence of a cap rock in the rift axis has not yet been proven; however, the high loss on ignition and the mineralogy in well Glc1 may indicate that the rocks are sufficiently altered to offer potential as a seal. If so, the rift margins would offer greater exploration potential than the rift center.

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Metagenome Assembly and Metagenome-Assembled Genome of “ Candidatus Aramenus sulfurataquae” from Thermal Sediments from the Los Azufres Volcanic Complex

Cited by 4 publications

References 15 publications

Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis

Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis

A Metagenome from a Steam Vent in Los Azufres Geothermal Field Shows an Abundance of Thermoplasmatales archaea and Bacteria from the Phyla Actinomycetota and Pseudomonadota

Natural hydrogen potential and basaltic alteration in the Asal–Ghoubbet rift, Republic of Djibouti

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