Metagenomic analysis of bacterial communities during estrous cycle in Bos indicus

Gohil, Purva; Patel, Kajal; Purohit, Kishan; Chavda, Dhruvil; Puvar, Apurvasinh; Suthar, Vishal; Patil, D. B.; Joshi, Madhavi; Joshi, Chaitanya

doi:10.1007/s11250-022-03119-5

Cited by 9 publications

(6 citation statements)

References 26 publications

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“…The human vaginal microbiota fluctuates over the menstrual cycle following hormone-mediated changes in glycogen availability, a key nutrient for Lactobacilli and other microbes 53,81,82 . Observations in other mammals are mixed; reproductive cycle-associated fluctuations have been reported in some non-human primates, cows, and rats [83][84][85][86][87] but not other nonhuman primates, horses, or mini-pigs [88][89][90][91] . Unlike conventional mice 26 , we observed correlations between estrous stage and vaginal communities, particularly Escherichia-dominant h mCST III-a, in HMb mice.…”

Section: Discussionmentioning

confidence: 99%

Vaginal microbial dynamics and pathogen colonization in a humanized microbiota mouse model

Mejia,

Mercado-Evans,

Zulk

et al. 2023

npj Biofilms Microbiomes

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Vaginal microbial composition is associated with differential risk of urogenital infection. Although Lactobacillus spp. are thought to confer protection against infection, the lack of in vivo models resembling the human vaginal microbiota remains a prominent barrier to mechanistic discovery. Using 16S rRNA amplicon sequencing of C57BL/6J female mice, we found that vaginal microbial composition varies within and between colonies across three vivaria. Noting vaginal microbial plasticity in conventional mice, we assessed the vaginal microbiome of humanized microbiota mice (HMbmice). Like the community structure in conventional mice, HMbmice vaginal microbiota clustered into community state types but, uniquely, HMbmice communities were frequently dominated by Lactobacillus or Enterobacteriaceae. Compared to conventional mice, HMbmice were less susceptible to uterine ascension by urogenital pathobionts group B Streptococcus (GBS) and Prevotella bivia. Although Escherichia and Lactobacillus both correlated with the absence of uterine GBS, vaginal pre-inoculation with exogenous HMbmouse-derived E. coli, but not Ligilactobacillus murinus, reduced vaginal GBS burden. Overall, HMbmice serve as a useful model to elucidate the role of endogenous microbes in conferring protection against urogenital pathogens.

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Section: Discussionmentioning

confidence: 99%

Vaginal microbial dynamics and pathogen colonization in a humanized microbiota mouse model

Mejia,

Mercado-Evans,

Zulk

et al. 2023

npj Biofilms Microbiomes

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“…Bacterial diversity differed significantly between the luteal and follicular phases of the Indian buffalo reproductive cycle. During the luteal phase, Micrococcus was more abundant, whereas Massilia and Methylobacter were more prevalent during the follicular phase [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Correlation between microbial characteristics and reproductive status of the yak uterus based on macrogenomic analysis

Wang,

Zeng

et al. 2024

BMC Vet Res

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Introduction This study aimed to investigate the microbial characteristics of yak uteri collected using intrauterine cotton swabs (CS) during different reproductive stages and the correlation of these microbial characteristics with reproductive status. Methods We used a macrogenomic approach to analyze the functional aspects of different microorganisms in samples collected during the pre-estrus, estrus, late estrus, and diestrus stages. Results The results revealed the presence of 1293 microbial genera and 3401 microbial species in the uteri of yaks at different reproductive stages. The dominant bacterial species varied across the different periods, with Micrococcus and Proteus being dominant during pre-estrus; Pseudomonas, Clostridium, Flavobacterium, Bacillus, and Staphylococcus during estrus; Acinetobacter, Bacillus and Proteus during late estrus; and Pseudomonas, Escherichia coli, and Proteus during diestrus. Discussion The primary functions of these bacteria are enriched in various metabolic pathways, including carbohydrate and amino acid metabolism, intracellular transport and secretion, post-translational protein modification, and drug resistance. These findings suggest that the microbial diversity in the uterus of yaks plays a crucial role in reproductive regulation and can help prevent reproductive tract-related diseases.

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“…In humans, the vaginal microbiota composition fluctuates over the menstrual cycle likely due to steroid hormone-mediated changes in glycogen availability, a key nutrient source for Lactobacilli and other microbes (62,74,75). Observations in other mammals are mixed; reproductive cycle-associated fluctuations have been reported in some studies of non-human primates, bovine species, and rats (76)(77)(78)(79)(80) but not in other non-human primates, horses, or mini-pigs (81)(82)(83)(84). Like conventional C57BL/6J mice (24), we did not observe a strong influence of estrous stage on vaginal microbial compositions or individual taxa in HMb mice.…”

Section: Discussionmentioning

confidence: 99%

Vaginal microbial dynamics and pathogen colonization in a humanized microbiota mouse model

Mejia

Mercado-Evans

Zulk

et al. 2023

Preprint

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Vaginal microbiota composition is associated with differential risk of urogenital infection. Although vaginal Lactobacillus spp. are thought to confer protection through acidification, bacteriocin production, and immunomodulation, lack of an in vivo model system that closely resembles the human vaginal microbiota remains a prominent barrier to mechanistic discovery. We performed 16S rRNA amplicon sequencing of wildtype C57BL/6J mice, commonly used to study pathogen colonization, and found that the vaginal microbiome composition varies highly both within and between colonies from three distinct vivaria. Because of the strong influence of environmental exposure on vaginal microbiome composition, we assessed whether a humanized microbiota mouse (HMbmice) would model a more human-like vaginal microbiota. Similar to humans and conventional mice, HMbmice vaginal microbiota clustered into five community state types (hmCST). Uniquely, HMbmice vaginal communities were frequently dominated by Lactobacilli or Enterobacteriaceae. Compared to genetically-matched conventional mice, HMbmice were less susceptible to uterine ascension by urogenital pathobionts group B Streptococcus (GBS) and Prevotella bivia, but no differences were observed with uropathogenic E. coli. Specifically, vaginal Enterobacteriaceae and Lactobacillus were associated with the absence of uterine GBS. Anti-GBS activity of HMbmice vaginal E. coli and L. murinus isolates, representing Enterobacteriaceae and Lactobacillus respectively, were characterized in vitro and in vivo. Although L. murinus reduced GBS growth in vitro, vaginal pre-inoculation with HMbmouse-derived E. coli, but not L. murinus, conferred protection against vaginal GBS burden. Overall, the HMbmice are an improved model to elucidate the role of endogenous microbes in conferring protection against urogenital pathogens.

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Metagenomic analysis of bacterial communities during estrous cycle in Bos indicus

Cited by 9 publications

References 26 publications

Vaginal microbial dynamics and pathogen colonization in a humanized microbiota mouse model

Vaginal microbial dynamics and pathogen colonization in a humanized microbiota mouse model

Correlation between microbial characteristics and reproductive status of the yak uterus based on macrogenomic analysis

Vaginal microbial dynamics and pathogen colonization in a humanized microbiota mouse model

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