2022
DOI: 10.1101/2022.09.16.508313
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Metagenomic analysis of concrete bridge reveals a microbial community dominated by halophilic Bacteria and Archaea

Abstract: Concrete hosts a small but diverse microbiome that changes over time. Shotgun metagenomic sequencing would enable assessment of both diversity and function of the microbial community in concrete, but because the biomass in concrete is so low, this analysis is highly affected by laboratory contamination. Here, we demonstrate improved DNA extraction from concrete, and show that this method provides DNA of sufficient quality and quantity to do shotgun metagenomic sequencing. DNA was extracted from a sample of con… Show more

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