Metagenomic analysis of microbial communities across a transect from low to highly hydrocarbon‐contaminated soils in King George Island, Maritime Antarctica

Jurelevicius, Diogo; Pereira, Raphael da Silva; Mota, Fábio Faria da; Cury, José; Oliveira, Ivan Cardoso de; Rosado, Alexandre Soares; Mason, Olivia U.; Jansson, Janet K.; Seldin, Lucy

doi:10.1111/gbi.12472

Cited by 14 publications

(7 citation statements)

References 50 publications

(66 reference statements)

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“…These bacteria groups known to be involved in nitrogen fixation 42 . These results are in consistent with other studies that observed dominance of Proteobacteria in agricultural soils 43,44 . Upon treatment with MONPs, there was no change in the abundance of Proteobacteria while Bacteriodetes generally increased after 30 days of exposure.…”

Section: Discussionsupporting

confidence: 94%

Alteration of Soil Bacteriome by Prolonged Exposure to Metal Oxide Nanoparticles

Egboluche

Wachira

2022

Preprint

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Metal oxide nanoparticles (MONPs) have found applications in many industrial and consumer products and are inevitably released into the environment, including soil. Soils host diverse microorganisms that are integral to ecosystem function including regulating plant growth. In this study, the influence of Cu2O, Fe3O4 and Ag2O NPs on soil microbial communities was assessed. Microbial community diversity and compositional structure was characterized using quantitative PCR and 16S rRNA gene sequencing. MONPs altered soil bacteria community composition by causing significant reduction in bacterial diversity and change in bacterial abundance. Soils with Cu2O and Ag2O NPs treatments significantly reduce bacterial diversity accompanied by shifts at the Class and Phylum taxonomic levels toward bacteria groups responsible for chitin degradation (Bacteriodetes) and nitrogen fixation (alpha-Proteobacteria). Response of bacterial communities to MONPs exposure is dependent on the exposure time and type of MONPs used. While the mechanisms underlying these observations remain to be elucidated, it is proposed that the known antimicrobial properties of Cu2O and Ag2O NPs cause reduced growth and viability of some bacteria taxa.

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Section: Discussionsupporting

confidence: 94%

Alteration of Soil Bacteriome by Prolonged Exposure to Metal Oxide Nanoparticles

Egboluche

Wachira

2022

Preprint

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“…4 ). Proteobacteria and Actinobacteria have been reported to be the most abundant phyla in both Antarctic soils 34 and soils contaminated with PAHs 35 . Figure 5 a,b show all the genera belonging to the Proteobacteria and Actinobacteria phyla, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Sulbaran-Bracho et al 6 determined the community composition of bacteria during diesel biodegradation by consortium LR-10 isolated from Antarctic rhizosphere soil and found that the dominant bacterial genera were Pseudomonas , Candidimonas , Rhodanobacter , Renibacterium , Pseudoarthrobacter , and Frateuria . Jurelevicius et al 35 investigated the microbial communities present in hydrocarbon-contaminated soils from King George Island, Antarctica. They found positive correlations between the abundances of Cytophaga , Methyloversatilis , Polaromonas , and Williamsia and the concentrations of total petroleum hydrocarbons and/or PAHs.…”

Section: Resultsmentioning

confidence: 99%

Pseudomonas and Pseudarthrobacter are the key players in synergistic phenanthrene biodegradation at low temperatures

Naloka,

Kuntaveesuk,

Muangchinda

et al. 2024

Sci Rep

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Hydrocarbon contamination, including contamination with polycyclic aromatic hydrocarbons (PAHs), is a major concern in Antarctica due to the toxicity, recalcitrance and persistence of these compounds. Under the Antarctic Treaty, nonindigenous species are not permitted for use in bioremediation at polluted sites in the Antarctic region. In this study, three bacterial consortia (C13, C15, and C23) were isolated from Antarctic soils for phenanthrene degradation. All isolated bacterial consortia demonstrated phenanthrene degradation percentages ranging from 45 to 85% for 50 mg/L phenanthrene at 15 ℃ within 5 days. Furthermore, consortium C13 exhibited efficient phenanthrene degradation potential across a wide range of environmental conditions, including different temperature (4–30 ℃) and water availability (without polyethylene glycol (PEG) 6000 or 30% PEG 6000 (w/v)) conditions. Sequencing analysis of 16S rRNA genes revealed that Pseudomonas and Pseudarthrobacter were the dominant genera in the phenanthrene-degrading consortia. Moreover, six cultivable strains were isolated from these consortia, comprising four strains of Pseudomonas, one strain of Pseudarthrobacter, and one strain of Paeniglutamicibacter. These isolated strains exhibited the ability to degrade 50 mg/L phenanthrene, with degradation percentages ranging from 4 to 22% at 15 ℃ within 15 days. Additionally, the constructed consortia containing Pseudomonas spp. and Pseudarthrobacter sp. exhibited more effective phenanthrene degradation (43–52%) than did the individual strains. These results provide evidence that Pseudomonas and Pseudarthrobacter can be potential candidates for synergistic phenanthrene degradation at low temperatures. Overall, our study offers valuable information for the bioremediation of PAH contamination in Antarctic environments.

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“…Metagenomic analysis is a valuable tool for environmental monitoring, enabling comprehensive identification of microbial communities in specific habitats ( Feng et al, 2018 ; Jurelevicius et al, 2022 ). In this study, bacterial communities in sediment samples were analyzed using amplicon NGS targeting the V3 and V4 regions of the 16S rRNA gene.…”

Section: Discussionmentioning

confidence: 99%

Unraveling the shift in bacterial communities profile grown in sediments co-contaminated with chlorolignin waste of pulp-paper mill by metagenomics approach

Kumar,

Ameen,

Verma

2024

Front. Microbiol.

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Pulp-paper mills (PPMs) are known for consistently generating a wide variety of pollutants, that are often unidentified and highly resistant to environmental degradation. The current study aims to investigate the changes in the indigenous bacterial communities profile grown in the sediment co-contaminated with organic and inorganic pollutants discharged from the PPMs. The two sediment samples, designated PPS-1 and PPS-2, were collected from two different sites. Physico-chemical characterization of PPS-1 and PPS-2 revealed the presence of heavy metals (mg kg−1) like Cu (0.009–0.01), Ni (0.005–0.002), Mn (0.078–0.056), Cr (0.015–0.009), Pb (0.008–0.006), Zn (0.225–0.086), Fe (2.124–0.764), Al (3.477–22.277), and Ti (99.792–45.012) along with high content of chlorophenol, and lignin. The comparative analysis of organic pollutants in sediment samples using gas chromatography–mass spectrometry (GC–MS) revealed the presence of major highly refractory compounds, such as stigmasterol, β-sitosterol, hexadecanoic acid, octadecanoic acid; 2,4-di-tert-butylphenol; heptacosane; dimethyl phthalate; hexachlorobenzene; 1-decanol,2-hexyl; furane 2,5-dimethyl, etc in sediment samples which are reported as a potential toxic compounds. Simultaneously, high-throughput sequencing targeting the V3–V4 hypervariable region of the 16S rRNA genes, resulted in the identification of 1,249 and 1,345 operational taxonomic units (OTUs) derived from a total of 115,665 and 119,386 sequences read, in PPS-1 and PPS-2, respectively. Analysis of rarefaction curves indicated a diversity in OTU abundance between PPS-1 (1,249 OTUs) and PPS-2 (1,345 OTUs). Furthermore, taxonomic assignment of metagenomics sequence data showed that Proteobacteria (55.40%; 56.30%), Bacteoidetes (11.30%; 12.20%), and Planctomycetes (5.40%; 4.70%) were the most abundant phyla; Alphproteobacteria (20.50%; 23.50%), Betaproteobacteria (16.00%; 12.30%), and Gammaproteobacteria were the most recorded classes in PPS-1 and PPS-2, respectively. At the genus level, Thiobacillus (7.60%; 4.50%) was the most abundant genera grown in sediment samples. The results indicate significant differences in both the diversity and relative abundance of taxa in the bacterial communities associated with PPS-2 when compared to PPS-1. This study unveils key insights into contaminant characteristics and shifts in bacterial communities within contaminated environments. It highlights the potential for developing efficient bioremediation techniques to restore ecological balance in pulp-paper mill waste-polluted areas, stressing the importance of identifying a significant percentage of unclassified genera and species to explore novel genes.

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Metagenomic analysis of microbial communities across a transect from low to highly hydrocarbon‐contaminated soils in King George Island, Maritime Antarctica

Cited by 14 publications

References 50 publications

Alteration of Soil Bacteriome by Prolonged Exposure to Metal Oxide Nanoparticles

Alteration of Soil Bacteriome by Prolonged Exposure to Metal Oxide Nanoparticles

Pseudomonas and Pseudarthrobacter are the key players in synergistic phenanthrene biodegradation at low temperatures

Unraveling the shift in bacterial communities profile grown in sediments co-contaminated with chlorolignin waste of pulp-paper mill by metagenomics approach

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