Metagenomic analysis of the bacterial microbiota linked to the traditional Algerian date product “Btana”

Abekhti, Abdelkader; Taminiau, Bernard; Kihal, Mebrouk; Daube, Georges

doi:10.1007/s13213-015-1084-3

Cited by 6 publications

(7 citation statements)

References 38 publications

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“…To overcome this, population in southern Algeria transform dates to a traditional fermented product called Btana. Our previous study confirmed that Btana is an interesting method that prevents date spoilage for a period up to 2 years (Abekhti 2015). This product is manufactured according to two methods both inspired by traditional knowledge and have been thoroughly described in our previous study (Abekhti et al 2013).…”

Section: Introductionsupporting

confidence: 54%

Metabarcoding analysis and fermentation performance of the dominant fungal microbiota associated with the Algerian traditional date product “Btana”

et al. 2021

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The study highlights the fungal diversity of the traditional Algerian date's product "Btana" prepared with direct Btana method (DBM) and undirect Btana method (UBM). Btana fungal populations were analyzed through 28S metabarcoding. Data treatment resulted in 122,997 reads representing three Phyla in which 76% reads (46 OTUs) belong to Ascomycota phylum. Zygosaccharomyces rouxii was the most prevailed species accounting for 35.40% of the total population. Similarity percentage analysis revealed a low level of resemblance in species in each of the two Btana types (DBM: 17.26%, UBM: 16.87). According to HPLC analysis, lactate was detected in nine samples within a range of 0.87-23.06 g/100g. Culture plating and subsequent D1/ D2 domain of 28s DNA analysis showed the prevalence of Z. rouxii. Fermentation of non-renewed date medium revealed a high ethanol production (21.31 ± 2.89 g/100g) by Lachancea thermotolerans and 5.87 g/100g of lactates by Kluyveromyces delphensis. Enzymatic assay revealed a high esterase (C4) and naphtol-AS-BI-phosphohydrolase activity by L. thermotolerans, K. delphensis, and Pichia subpelliculosa, while a high level of α-fucosidase was recorded for L. thermotolerans and P. subpelliculosa. The current results demonstrated that the traditional date product Btana is a promising source for yeasts useful in production of value-added products like bioethanol and lactic acid using low-income date cultivars.

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Section: Introductionsupporting

confidence: 54%

Metabarcoding analysis and fermentation performance of the dominant fungal microbiota associated with the Algerian traditional date product “Btana”

et al. 2021

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“…The devastating impact of mycotoxins on the immune system of both adults and children is of major public health concern, while newborns are more vulnerable to mycotoxins than adults due to their smaller body weight and less acidic stomach [104]. Abass et al (2013) examined the most common species of fungi in six different date palm cultivars in Iraq, including Um Al-Dihin, Shwaythee, Breem, Barhi, Hilawi, and Al-Sayer.…”

Section: Microbiological Contamination Of Datesmentioning

confidence: 99%

An Overview of Date (Phoenix dactylifera) Fruits as an Important Global Food Resource

Al-Karmadi,

Okoh

2024

Foods

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Dates are the fruits of the date palm belonging to the Arecaceae family; they comprise over 2500 species and 200 genera and constitute an essential part of the daily diet worldwide, with beneficial nutritional, health, and economic values. Several varieties of date palm (Phoenix dactylifera) fruit exist globally, especially in hot and humid regions. This review is an overview of date palms as a significant global food resource, including their historical significance, nutritional composition, cultivation practices, economic importance, and health benefits. The historical journey of dates goes back to ancient civilizations where they were revered for their richness in essential nutrients and natural sweetness. Today, dates are a vital crop in arid regions, contributing substantially to the agricultural economy and livelihoods of communities. This paper further explores the cultivation techniques employed to enhance date production. Furthermore, the nutritional composition of dates is analyzed in detail, highlighting their high content of vitamins, minerals, dietary fibers, and antioxidants. These attributes make dates a delicious treat and a valuable nutritional component, offering numerous health benefits. The potential health effects, including improved digestion, enhanced cardiovascular health, and increased energy levels, are discussed. Additionally, this paper delves into the economic significance of the date industry and its global trade.

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“…On the extracted DNA, PCR targeting tcdA and tcdB genes was performed using primers reported by Kouhsari et al, 2019 [30]. Then, PCR targeting 16S rDNA was performed according to the protocol explained by Abdelkader et al (2021) [31]. The length of the fragment is 1500 bp, and the universal primers are 16Sr (TAC-GGT-TAC-CTT-GTT-ACG-AC) and 16Sf (GAG-TTT-GAT-CMT-GGC-TCA-G).…”

Section: Confirmation C Difficile Isolates and Toxin Gene Detectionmentioning

confidence: 99%

Gut Microbiota Associated with Clostridioides difficile Carriage in Three Clinical Groups (Inflammatory Bowel Disease, C. difficile Infection and Healthcare Workers) in Hospital Field

Martinez,

Crevecoeur,

Thirion

et al. 2023

Microorganisms

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Clostridioides difficile is an anaerobic spore-forming Gram-positive bacterium. C. difficile carriage and 16S rDNA profiling were studied in three clinical groups at three different sampling times: inflammatory bowel disease (IBD) patients, C. difficile infection (CDI) patients and healthcare workers (HCWs). Diversity analysis was realized in the three clinical groups, the positive and negative C. difficile carriage groups and the three analysis periods. Concerning the three clinical groups, β-diversity tests showed significant differences between them, especially between the HCW group and IBD group and between IBD patients and CDI patients. The Simpson index (evenness) showed a significant difference between two clinical groups (HCWs and IBD). Several genera were significantly different in the IBD patient group (Sutterella, Agathobacter) and in the CDI patient group (Enterococcus, Clostridioides). Concerning the positive and negative C. difficile carriage groups, β-diversity tests showed significant differences. Shannon, Simpson and InvSimpson indexes showed significant differences between the two groups. Several genera had significantly different relative prevalences in the negative group (Agathobacter, Sutterella, Anaerostipes, Oscillospira) and the positive group (Enterococcus, Enterobacteriaceae_ge and Enterobacterales_ge). A microbiota footprint was detected in C. difficile-positive carriers. More experiments are needed to test this microbiota footprint to see its impact on C. difficile infection.

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Metagenomic analysis of the bacterial microbiota linked to the traditional Algerian date product “Btana”

Cited by 6 publications

References 38 publications

Metabarcoding analysis and fermentation performance of the dominant fungal microbiota associated with the Algerian traditional date product “Btana”

Metabarcoding analysis and fermentation performance of the dominant fungal microbiota associated with the Algerian traditional date product “Btana”

An Overview of Date (Phoenix dactylifera) Fruits as an Important Global Food Resource

Gut Microbiota Associated with Clostridioides difficile Carriage in Three Clinical Groups (Inflammatory Bowel Disease, C. difficile Infection and Healthcare Workers) in Hospital Field

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