Metagenomic analysis reveals antibiotic resistance genes in the bovine rumen

Jing, Ruoxi; Yan, Yueyang

doi:10.1016/j.micpath.2020.104350

Cited by 20 publications

(12 citation statements)

References 33 publications

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“…The hostmicrobe interactions are crucial for the expression of ARG, colonization, and communication (Zhou et al, 2010;Capra and Laub, 2012). It has been demonstrated that tetracycline ARG are widely present in microorganisms (Roberts, 2005), including the rumen of ruminants (El and Dunlop, 2018;Jing and Yan, 2020), which is consistent with the proportion of tetracycline ARGs observed in this study. macB, tetW, tetB (P), tetQ are the main tetracycline ARG in the rumen, and tetW is the most abundant gene, distributed in 28 rumen bacterial genomes, with high nucleotide sequence similarity (Sabino et al, 2019).…”

Section: Discussionsupporting

confidence: 89%

Citrus flavonoid extracts alter the profiling of rumen antibiotic resistance genes and virulence factors of dairy cows

Yu,

Li,

Zhao

et al. 2023

Front. Microbiol.

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Citrus flavonoid extracts (CFE) have the potential to reduce rumen inflammation, improve ruminal function, and enhance production performance in ruminants. Our previous studies have investigated the effects of CFE on the structure and function of rumen microbiota in dairy cows. However, it remains unclear whether CFE affects the prevalence of antibiotic resistance genes (ARG) and virulence factors genes (VFG) in the rumen. Therefore, metagenomics was used to identify the rumen ARG and VFG in lactating dairy cows fed with CFE diets. The results showed that CFE significantly reduced the levels of Multidrug and Antiphagocytosis in the rumen (p < 0.05) and increased the levels of Tetracycline, Iron uptake system, and Magnesium uptake system (p < 0.05). Furthermore, the changes were found to have associations with the phylum Lentisphaerae. It was concluded that CFE could be utilized as a natural plant product to regulate virulence factors and antibiotic resistance of rumen microbiota, thereby improving rumen homeostasis and the health of dairy cows.

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Section: Discussionsupporting

confidence: 89%

Citrus flavonoid extracts alter the profiling of rumen antibiotic resistance genes and virulence factors of dairy cows

Yu,

Li,

Zhao

et al. 2023

Front. Microbiol.

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“…Bacitracin interferes with peptidoglycan synthesis by inhibiting the dephosphorylation of lipid carriers. A previous study showed that bacitracin, a common in-feed antimicrobial, was abundantly present in bovine GMCs ( 83 ). In addition, our results indicated genes associated with resistance to vancomycin, a “last resort” broad-spectrum antibiotic, was the third most detected antibiotic resistance gene (ARG) type.…”

Section: Discussionmentioning

confidence: 99%

Unveiling the Gut Microbiota and Resistome of Wild Cotton Mice, Peromyscus gossypinus , from Heavy Metal- and Radionuclide-Contaminated Sites in the Southeastern United States

et al. 2021

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“…For the first time, our results point to this bacterium as a possible and novel predictor MDRB biomarker in SARS-CoV-2 infection. Interestingly, previous metagenomic studies have identified this family as genomes harbouring antibiotic resistance genes [ 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota Composition Can Predict Colonization by Multidrug-Resistant Bacteria in SARS-CoV-2 Patients in Intensive Care Unit: A Pilot Study

et al. 2023

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The SARS-CoV-2 infection has increased the number of patients entering Intensive Care Unit (ICU) facilities and antibiotic treatments. Concurrently, the multi-drug resistant bacteria (MDRB) colonization index has risen. Considering that most of these bacteria are derived from gut microbiota, the study of its composition is essential. Additionally, SARS-CoV-2 infection may promote gut dysbiosis, suggesting an effect on microbiota composition. This pilot study aims to determine bacteria biomarkers to predict MDRB colonization risk in SARS-CoV-2 patients in ICUs. Seventeen adult patients with an ICU stay >48 h and who tested positive for SARS-CoV-2 infection were enrolled in this study. Patients were assigned to two groups according to routine MDRB colonization surveillance: non-colonized and colonized. Stool samples were collected when entering ICUs, and microbiota composition was determined through Next Generation Sequencing techniques. Gut microbiota from colonized patients presented significantly lower bacterial diversity compared with non-colonized patients (p < 0.05). Microbiota in colonized subjects showed higher abundance of Anaerococcus, Dialister and Peptoniphilus, while higher levels of Enterococcus, Ochrobactrum and Staphylococcus were found in non-colonized ones. Moreover, LEfSe analysis suggests an initial detection of Dialister propionicifaciens as a biomarker of MDRB colonization risk. This pilot study shows that gut microbiota profile can become a predictor biomarker for MDRB colonization in SARS-CoV-2 patients.

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Metagenomic analysis reveals antibiotic resistance genes in the bovine rumen

Cited by 20 publications

References 33 publications

Citrus flavonoid extracts alter the profiling of rumen antibiotic resistance genes and virulence factors of dairy cows

Citrus flavonoid extracts alter the profiling of rumen antibiotic resistance genes and virulence factors of dairy cows

Unveiling the Gut Microbiota and Resistome of Wild Cotton Mice, Peromyscus gossypinus , from Heavy Metal- and Radionuclide-Contaminated Sites in the Southeastern United States

Gut Microbiota Composition Can Predict Colonization by Multidrug-Resistant Bacteria in SARS-CoV-2 Patients in Intensive Care Unit: A Pilot Study

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