Metagenomic applications in microbial diversity, bioremediation, pollution monitoring, enzyme and drug discovery. A review

Datta, Saptashwa; Rajnish, K. Narayanan; Samuel, Melvin S.; Pugazlendhi, Arivalagan; Ethiraj, Selvarajan

doi:10.1007/s10311-020-01010-z

Cited by 98 publications

(37 citation statements)

References 108 publications

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“…Environmental pollution is a daunting challenge to face in the modern world [ 1 ]. Pollutants from land and water are foremost in reducing the quality of life and human health [ 2 ]. Textile dyes released from textile industries into the water lead to several hazards to the environment, mainly due to their recalcitrant and toxic nature [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

et al. 2020

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This study focuses on the development of a nanosupport based on halloysite nanotubes (HNTs), Fe3O4 nanoparticles (NPs), and thiolated chitosan (CTs) for laccase immobilization. First, HNTs were modified with Fe3O4 NPs (HNTs-Fe3O4) by the coprecipitation method. Then, the HNTs-Fe3O4 surface was tuned with the CTs (HNTs-Fe3O4-CTs) by a simple refluxing method. Finally, the HNTs- Fe3O4-CTs surface was thiolated (-SH) (denoted as; HNTs- Fe3O4-CTs-SH) by using the reactive NHS-ester reaction. The thiol-modified HNTs (HNTs- Fe3O4-CTs-SH) were characterized by FE-SEM, HR-TEM, XPS, XRD, FT-IR, and VSM analyses. The HNTs-Fe3O4-CTs-SH was applied for the laccase immobilization. It gave excellent immobilization of laccase with 100% activity recovery and 144 mg/g laccase loading capacity. The immobilized laccase on HNTs-Fe3O4-CTs-SH (HNTs-Fe3O4-CTs-S-S-Laccase) exhibited enhanced biocatalytic performance with improved thermal, storage, and pH stabilities. HNTs-Fe3O4-CTs-S-S-Laccase gave outstanding repeated cycle capability, at the end of the 15th cycle, it kept 61% of the laccase activity. Furthermore, HNTs-Fe3O4-CTs-S-S-Laccase was applied for redox-mediated removal of textile dye DR80 and pharmaceutical compound ampicillin. The obtained result marked the potential of the HNTs-Fe3O4-CTs-S-S-Laccase for the removal of hazardous pollutants. This nanosupport is based on clay mineral HNTs, made from low-cost biopolymer CTs, super-magnetic in nature, and can be applied in laccase-based decontamination of environmental pollutants. This study also gave excellent material HNTs-Fe3O4-CTs-SH for other enzyme immobilization processes.

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Section: Introductionmentioning

confidence: 99%

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

et al. 2020

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“…This has great implications when considering strains challenging to isolate or cultivate, such as strains from extreme environments and symbionts of marine organisms [ 16 , 58 ]. The revolution in this approach encompassing the phenotypic and homology DNA screening strategies in situ has been ameliorated by the advancement of next-generation sequencing (NGS) technologies [ 59 , 60 , 61 ]. By directly capturing eDNA from the environment and subsequently identifying, isolating, and expressing BGCs in a heterologous host, metagenomics has the potential to bring biosynthetic diversity from the environment into drug discovery pipelines.…”

Section: Bioinformatics- and Genomics-driven Discoverymentioning

confidence: 99%

Recent Advances in Discovery of Lead Structures from Microbial Natural Products: Genomics- and Metabolomics-Guided Acceleration

Sukmarini

2021

Molecules

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Natural products (NPs) are evolutionarily optimized as drug-like molecules and remain the most consistently successful source of drugs and drug leads. They offer major opportunities for finding novel lead structures that are active against a broad spectrum of assay targets, particularly those from secondary metabolites of microbial origin. Due to traditional discovery approaches’ limitations relying on untargeted screening methods, there is a growing trend to employ unconventional secondary metabolomics techniques. Aided by the more in-depth understanding of different biosynthetic pathways and the technological advancement in analytical instrumentation, the development of new methodologies provides an alternative that can accelerate discoveries of new lead-structures of natural origin. This present mini-review briefly discusses selected examples regarding advancements in bioinformatics and genomics (focusing on genome mining and metagenomics approaches), as well as bioanalytics (mass-spectrometry) towards the microbial NPs-based drug discovery and development. The selected recent discoveries from 2015 to 2020 are featured herein.

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“…Accumulate more than 1000 mg As kg −1 in DW tissue, Max accumulation Cd 5931 µ g/g DW. 3500 µ g/g DW Cr [79,80] Biodiversity is a point that needs to be explored in water phytoremediation systems, since diversity may infer different metabolic pathways, resistance and mobilization of HM characteristics in plants [81][82][83]. Since there are several diseases associated with these pollutants, and each of them may vary in its bioavailability and chemical structure, different mechanisms and plants have to be used to remove these pollutants.…”

Section: Wolffia Globosamentioning

confidence: 99%

Advances and Applications of Water Phytoremediation: A Potential Biotechnological Approach for the Treatment of Heavy Metals from Contaminated Water

Delgado-González

Madariaga-Navarrete

Fernández-Cortés

et al. 2021

IJERPH

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Potable and good-quality drinking water availability is a serious global concern, since several pollution sources significantly contribute to low water quality. Amongst these pollution sources, several are releasing an array of hazardous agents into various environmental and water matrices. Unfortunately, there are not very many ecologically friendly systems available to treat the contaminated environment exclusively. Consequently, heavy metal water contamination leads to many diseases in humans, such as cardiopulmonary diseases and cytotoxicity, among others. To solve this problem, there are a plethora of emerging technologies that play an important role in defining treatment strategies. Phytoremediation, the usage of plants to remove contaminants, is a technology that has been widely used to remediate pollution in soils, with particular reference to toxic elements. Thus, hydroponic systems coupled with bioremediation for the removal of water contaminants have shown great relevance. In this review, we addressed several studies that support the development of phytoremediation systems in water. We cover the importance of applied science and environmental engineering to generate sustainable strategies to improve water quality. In this context, the phytoremediation capabilities of different plant species and possible obstacles that phytoremediation systems may encounter are discussed with suitable examples by comparing different mechanistic processes. According to the presented data, there are a wide range of plant species with water phytoremediation potential that need to be studied from a multidisciplinary perspective to make water phytoremediation a viable method.

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Metagenomic applications in microbial diversity, bioremediation, pollution monitoring, enzyme and drug discovery. A review

Cited by 98 publications

References 108 publications

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

Recent Advances in Discovery of Lead Structures from Microbial Natural Products: Genomics- and Metabolomics-Guided Acceleration

Advances and Applications of Water Phytoremediation: A Potential Biotechnological Approach for the Treatment of Heavy Metals from Contaminated Water

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