Metagenomic Approach to Bacterial Diversity and Lipolytic Enzymes’ Genes from a Steam Soil of Los Humeros Geothermal Field (Puebla, México)

Mora-González, Paola Carolina; Espinosa-Luna, Graciela; Ramírez-Higuera, Abril; Peña-Montes, Carolina; Valerio‐Alfaro, Gerardo; Sánchez-Flores, Alejandro; Quintana‐Castro, Rodolfo; Sánchez-Otero, María-Guadalupe; Oliart-Ros, Rosa María

doi:10.1080/01490451.2020.1852452

Cited by 2 publications

(3 citation statements)

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“…In a previous investigation, our research group reported a high percentage of genes coding for non-characterized lipolytic enzymes in the metagenome obtained from a soil sample from the "Los Humeros" geothermal field (15 cm depth, 80 • C), enriched in a high-oleic acid safflower oil medium [33]. In order to identify new potential polyester hydrolases in the metagenome, we designed probes directed to conserved motifs of polyester hydrolases with experimentally confirmed hydrolytic activity, reported structural data and biochemical characterization (Table 1).…”

Section: In Silico Probe Designmentioning

confidence: 99%

“…In order to look for new enzymes with the ability to degrade polyesters we analyzed the metagenomic library (5.3 GB, 109,000 contigs) of a soil sample obtained from a steaming location at "Los Humeros" geothermal field (15 cm depth, 80 • C) that was enriched in a high-oleic acid safflower oil medium [33]. The search was undertaken using probes that were designed to specifically recognize the amino acids' sequences of conserved motifs of polyester hydrolases with experimentally confirmed hydrolytic activity, reported structural data and biochemical characterization [48].…”

Section: In Silico Probe Designmentioning

confidence: 99%

“…Recently, our research group investigated, by a whole-genome shotgun approach, the microbial diversity and the presence of lipolytic enzyme genes from steam soil of "Los Humeros" geothermal field, located in the Trans-Mexican volcanic belt and with surface temperatures between 50 • C and 90 • C. A high percentage of non-characterized lipolytic enzyme genes was observed in the metagenome of an enrichment culture of soil in an oil-rich medium, which suggest the presence of biotechnologically promising organisms and enzymes that deserve to be investigated [33].…”

Section: Introductionmentioning

confidence: 99%

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Selection of Putative Polyester Hydrolases from the Metagenome of Los Humeros Geothermal Field by Means of In Silico Probes

Solis-Palacios,

Espinosa-Luna,

Peña-Montes

et al. 2024

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Hydrolases are the most popular enzymes, and among the most valuable in biotechnological applications. Some hydrolases, such as lipases, esterases, proteases, cellulases and amylases, are used in the food industry and the production of biopharmaceuticals, biofuels, biopolymers and detergents. Of special interest are those obtained from thermophilic microorganisms. Although there is great microbial diversity in extreme environments, the investigations aimed at detecting and isolating enzymes with potential for polyester degradation such as polyethylene terephthalate (PET) are limited. In this work, we explored the metagenomic library of an oil-enriched soil sample from the “Los Humeros” geothermal field by means of in silico probes in search for enzymes potentially able to degrade polyesters. Using conserved motifs and activity-relevant sites of reported polyester hydrolases, we designed probes that allowed us to identify 6 potential polyester hydrolases in the metagenome. Three-dimensional structure prediction revealed a canonical α/β fold and a cap covering the active site of the enzymes. The catalytic triads were composed of Ser, His and Asp. Structural comparison, substrate binding site analysis and molecular docking suggested their potential as polyester hydrolases, particularly cutinases and PETases. An enzyme, REC98271, was cloned, expressed and characterized, showing thermophilic properties and preference for short-chain substrates. These findings contribute to our understanding of enzyme diversity in “Los Humeros” metagenome and their potential applications in biodegradation and recycling processes.

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Section: In Silico Probe Designmentioning

confidence: 99%

Section: In Silico Probe Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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