2018
DOI: 10.1099/mgen.0.000212
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Metagenomic assembly of new (sub)polar Cyanobacteria and their associated microbiome from non-axenic cultures

Abstract: Cyanobacteria form one of the most diversified phyla of Bacteria. They are important ecologically as primary producers, for Earth evolution and biotechnological applications. Yet, Cyanobacteria are notably difficult to purify and grow axenically, and most strains in culture collections contain heterotrophic bacteria that were probably associated with Cyanobacteria in the environment. Obtaining cyanobacterial DNA without contaminant sequences is thus a challenging and time-consuming task. Here, we describe a me… Show more

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Cited by 27 publications
(27 citation statements)
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References 68 publications
(79 reference statements)
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“…For example, in Microcystis , a bloom-forming genus that produce mucilaginous colonies, Porphyrobacter , Rhodobacterales, Sphingomonadales, and Burkholderiales are also typically associated (Shi et al, 2009, 2012). Further, cyanobacterial associations with Flavobacterium and members of Sphingomonadaceae, Burkholderiales and Rhizobiales have been described from metagenomes of culture collections belonging to different cyanobacterial genera (Cornet et al, 2018). Similarly, Porphyrobacter and some members of the Family Rhodobacteraceae are known from associations with the cyanobacteria Microcoleus sp.…”
Section: Resultsmentioning
confidence: 99%
“…For example, in Microcystis , a bloom-forming genus that produce mucilaginous colonies, Porphyrobacter , Rhodobacterales, Sphingomonadales, and Burkholderiales are also typically associated (Shi et al, 2009, 2012). Further, cyanobacterial associations with Flavobacterium and members of Sphingomonadaceae, Burkholderiales and Rhizobiales have been described from metagenomes of culture collections belonging to different cyanobacterial genera (Cornet et al, 2018). Similarly, Porphyrobacter and some members of the Family Rhodobacteraceae are known from associations with the cyanobacteria Microcoleus sp.…”
Section: Resultsmentioning
confidence: 99%
“…In addition to cyanobacterial species with ribosomal protein sequences that are currently available on the GenBank database, our analyses included 20 previously unsequenced cyanobacteria and one genome previously sequenced and described by Lara et al (2017) and Cornet et al (2018) (Table 1). These were ordered from culture collections or enriched from environmental samples (See Supplementary Methods).…”
Section: Methodsmentioning
confidence: 99%
“…3): Hydrogenophaga sp. RAC07, which was isolated from the phycosphere of Chrysochromulina tobin, and a Burkholderiales microbiome obtained from non-axenic cyanobacterial cultures and an associated Burkholderiales microbiome 32,33 suggest that members of the RubisCO_form_IC-lake cluster are associated with photoautotrophic planktonic organisms. Furthermore, Hydrogenophaga sp.…”
Section: Resultsmentioning
confidence: 99%