Metagenomic assessment of the <i>Cebus apella</i> gut microbiota

Firrman, Jenni; Liu, LinShu; Tanes, Ceylan; Bittinger, Kyle; Mahalak, Karley K.; Rinaldi, William

doi:10.1002/ajp.23023

Cited by 6 publications

(8 citation statements)

References 56 publications

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“…Notably, the important human gut bacterium, Akkermansia [34], was not detected in the baboon gut. Therefore, Akkermansia was more humanspecific and thus absent in the baboons in our study, no Akkermansia has been reported in NHPs [35,36].…”

Section: Discussioncontrasting

confidence: 55%

The microbiome of captive hamadryas baboons

Trivedi

Brejnrod

et al. 2020

anim microbiome

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Background: The hamadryas baboon (Papio hamadryas) is a highly social primate that lives in complex multilevel societies exhibiting a wide range of group behaviors akin to humans. In contrast to the widely studied human microbiome, there is a paucity of information on the host-associated microbiomes of nonhuman primates (NHPs). Here, our goal was to understand the microbial composition throughout different body sites of cohabiting baboons. Results: We analyzed 170 oral, oropharyngeal, cervical, uterine, vaginal, nasal and rectal samples from 16 hamadryas baboons via 16S rRNA gene sequencing. Additionally, raw Miseq sequencing data from 1041 comparable publicly available samples from the human oral cavity, gut and vagina were reanalyzed using the same pipeline. We compared the baboon and human microbiome of the oral cavity, gut and vagina, showing that the baboon microbiome is distinct from the human. Baboon cohabitants share similar microbial profiles in their cervix, uterus, vagina, and gut. The oral cavity, gut and vagina shared more bacterial amplicon sequence variants (ASVs) in group living baboons than in humans. The shared ASVs had significantly positive correlations between most body sites, suggesting a potential bacterial exchange throughout the body. No significant differences in gut microbiome composition were detected within the maternity line and between maternity lines, suggesting that the offspring gut microbiota is shaped primarily through bacterial exchange among cohabitants. Finally, Lactobacillus was not so predominant in baboon vagina as in the human vagina but was the most abundant genus in the baboon gut. Conclusions: This study is the first to provide comprehensive analyses of the baboon microbiota across different body sites. We contrast this to human body sites and find substantially different microbiomes. This group of cohabitating baboons generally showed higher microbial diversity and remarkable similarities between body sites than were observed in humans. These data and findings from one group of baboons can form the basis of future microbiome studies in baboons and be used as a reference in research where the microbiome is expected to impact human modeling with baboons.

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Section: Discussioncontrasting

confidence: 55%

The microbiome of captive hamadryas baboons

Trivedi

Brejnrod

et al. 2020

anim microbiome

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“…Notably, the important human gut bacterium, Akkermansia [37], was not detected in the baboon gut. Therefore, Akkermansia was more like human-specific and thus absent in the baboons in our study, no Akkermansia has been reported in NHPs [38,39].…”

Section: Discussioncontrasting

confidence: 51%

The microbiome of captive hamadryas baboon

Trivedi

Brejnrod

et al. 2020

Preprint

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Hamadryas baboon is a highly social primate that lives in complex multilevel societies exhibiting a wide range of group behaviors akin to humans. Here, we report the comprehensive 16s rRNA gene analyses of groupliving baboon microbiota across different body sites. Additionally, we compared the baboon and human microbiome of the oral cavity, gut and vagina. Our analyses show that the baboon microbiome is distinct from the human and baboon cohabitants share similar microbial profiles in multiple body sites. The oral, gut and vagina shared more bacterial ASVs in group-living baboons than in humans. The shared ASVs in baboons had significantly positive correlations, suggesting a potential bacterial exchange throughout the body. No significant differences in baboon gut microbiome composition within the maternity line and between maternity lines were detected, suggesting that the offspring acquire their gut microbiota primarily through bacterial exchange among cohabitants. Besides, Lactobacillus was not so predominant in baboon vagina as in the human vagina but was the most abundant genus in baboon gut. These data and findings can form the basis of future microbiome studies in baboons and be used as a reference to research where the microbiome is expected to impact human modeling with baboons.

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“…16S rRNA sequencing was performed and proportional analysis of bacterial community composition at the family genus level showed that, as in previous studies on the Cebus apella microbiome, Streptococcaceae Streptococcus and Lactobacillaceae Lactobacillus are dominant members of the gut microbial community (data not shown). 36 Overall, there were many fluctuations in the microbial community found in the fecal samples throughout the experiment, as such, there were not any statistically significant differences in proportional abundance before and after SN Stevia. However, at the genus level we did observe an increase in the Comamonas, Granulicatella, Sphingobacterium, and the Wautersiella genera (data not shown).…”

Section: Sn Stevia and Erythritol Increases Butyric Acid But Does Not...mentioning

confidence: 83%

“…The animal was chosen according to the same standards as Firrman et al 2019. 36 Following a 1 week control period, the animal was fed 62 mg/kg Splenda Naturals plus Stevia per day for 2 weeks. Fecal samples were collected and frozen immediately at −80 °C.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Impact of Steviol Glycosides and Erythritol on the Human and Cebus apella Gut Microbiome

Mahalak

Firrman

Tomasula

et al. 2019

J. Agric. Food Chem.

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Leaf extracts of Stevia rebaudiana, composed of more than 10 steviol glycosides (SGs), are used as non-nutritive, table sugar (sucrose) alternatives due to their high level of sweetness and low caloric impact. They are often combined with the sugar alcohol erythritol to increase volume and reduce aftertaste. Little is known of the impact of sugar alternatives on the human gut microbiota in terms of the diversity, composition, and metabolic products. Testing of SGs and erythritol using six representatives of the gut microbiota in vitro found no impact on bacterial growth, yet treatment with erythritol resulted in an enhancement of butyric and pentanoic acid production when tested using a human gut microbial community. Furthermore, administration of SGs and erythritol to a Cebus apella model resulted in changes to the gut microbial structure and diversity. Overall, the study did not find a negative impact of SGs and erythritol on the gut microbial community.

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Metagenomic assessment of the Cebus apella gut microbiota

Cited by 6 publications

References 56 publications

The microbiome of captive hamadryas baboons

The microbiome of captive hamadryas baboons

The microbiome of captive hamadryas baboon

Impact of Steviol Glycosides and Erythritol on the Human and Cebus apella Gut Microbiome

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