Metagenomic Investigation of Idiopathic Meningoencephalomyelitis in Dogs

Hoon-Hanks, Laura L.; McGrath, Stephanie; Tyler, Kenneth L.; Owen, Christopher L.; Stenglein, Mark D.

doi:10.1111/jvim.14877

Cited by 28 publications

(19 citation statements)

References 37 publications

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“…Non‐infectious meningoencephalitis (NIME) is a term used to characterise inflammatory brain diseases for which no underlying infectious agent is identified and when an immune‐mediated basis is suspected 1–4. The terms meningoencephalitis of unknown origin (MUO)3 or meningoencephalitis of unknown aetiology are also commonly used as the pathophysiology of NIME is still poorly understood 5.…”

Section: Introductionmentioning

confidence: 99%

Low frequency of pre‐treatment and post‐treatment haematological abnormalities in dogs with non‐infectious meningoencephalitis treated with cytosine arabinoside and prednisolone

Keegan¹,

Rose²,

Khan

et al. 2019

Veterinary Record Open

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BackgroundCytosine arabinoside (CA) and prednisolone are drugs commonly used together in the management of canine non-infectious meningoencephalitis (NIME). The aim of this study was to report the haematological findings before and after CA and prednisolone treatment and identify any adverse haematological events in this clinical setting, following the veterinary cooperative oncology group established common terminology criteria for recording adverse events following administration of chemotherapy or biological antineoplastic therapy.ResultsWhile 48 patients with a presumptive diagnosis of NIME had pretreatment haematology results, only 12 patients met the inclusion criteria of also having post-treatment haematology results available for review after being treated with prednisolone and CA at a standard dose (200 mg/m2) in a single referral hospital in the UK. Forty-nine post-treatment haematology results were available for these 12 patients.ConclusionsFour adverse haematological events were identified in four patients. None of these events were convincingly attributable to CA administration.

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Section: Introductionmentioning

confidence: 99%

Low frequency of pre‐treatment and post‐treatment haematological abnormalities in dogs with non‐infectious meningoencephalitis treated with cytosine arabinoside and prednisolone

Keegan¹,

Rose²,

Khan

et al. 2019

Veterinary Record Open

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“…We did not identify any viral contigs in the two mule deer samples (brain and lymph node tissue.) Previous DNA-Seq [50] detected a herpesvirus in the mule deer. Herpesvirus is a dsDNA virus, which likely explains our inability to detect viral reads via dsRNA-Seq.…”

Section: Resultsmentioning

confidence: 99%

dsRNA-Seq: Identification of Viral Infection by Purifying and Sequencing dsRNA

Decker

Steiner

Hoon-Hanks

et al. 2019

Viruses

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RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed a method to identify RNA viruses that is based on the fact that RNA viruses produce double-stranded RNA (dsRNA) while replicating. Purifying and sequencing dsRNA from the total RNA isolated from infected tissue allowed us to recover dsRNA virus sequences and replicated sequences from single-stranded RNA (ssRNA) viruses. We refer to this approach as dsRNA-Seq. By assembling dsRNA sequences into contigs we identified full length or partial RNA viral genomes of varying genome types infecting mammalian culture samples, identified a known viral disease agent in laboratory infected mice, and successfully detected naturally occurring RNA viral infections in reptiles. Here, we show that dsRNA-Seq is a preferable method for identifying viruses in organisms that don’t have sequenced genomes and/or commercially available rRNA depletion reagents. In addition, a significant advantage of this method is the ability to identify replicated viral sequences of ssRNA viruses, which is useful for distinguishing infectious viral agents from potential noninfectious viral particles or contaminants.

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“…We did not identify any viral contigs in the two mule deer samples (brain and lymph node tissue.) Previous DNA-Seq [41] detected a herpesvirus in the mule deer. Herpesvirus is a dsDNA virus, which likely explains our inability to detect viral reads via dsRNA-Seq.…”

Section: Dsrna-seq Detects Rna Viruses Of Multiple Genome Types In Inmentioning

confidence: 99%

dsRNA-Seq: Identification of viral infection by purifying and sequencing dsRNA

Decker

Steiner²,

Hoon-Hanks

et al. 2019

Preprint

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RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed a method to identify RNA viruses that is based on the fact that all RNA viruses produce dsRNA while replicating. Purifying and sequencing dsRNA from total RNA isolated from infected tissue allowed us to recover replicating viral sequences. We refer to this approach as dsRNA-Seq. By assembling dsRNA sequences into contigs we identified full length RNA viruses of varying genome types infecting mammalian culture samples, identified a known viral disease agent in laboratory infected mice, and successfully detected naturally occurring RNA viral infections in reptiles. Here we show that dsRNA-Seq is a preferable method for identifying viruses in organisms that don't have sequenced genomes and/or commercially available rRNA depletion reagents.Similar to other metagenomic strategies, dsRNA-Seq has the potential to identify unknown viral disease agents that share little to no similarity to known viruses. However, the significant advantage of this method is the ability to identify replicated viral sequences, which is useful for distinguishing infectious viral agents from potential noninfectious viral particles or contaminants.

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Metagenomic Investigation of Idiopathic Meningoencephalomyelitis in Dogs

Cited by 28 publications

References 37 publications

Low frequency of pre‐treatment and post‐treatment haematological abnormalities in dogs with non‐infectious meningoencephalitis treated with cytosine arabinoside and prednisolone

Low frequency of pre‐treatment and post‐treatment haematological abnormalities in dogs with non‐infectious meningoencephalitis treated with cytosine arabinoside and prednisolone

dsRNA-Seq: Identification of Viral Infection by Purifying and Sequencing dsRNA

dsRNA-Seq: Identification of viral infection by purifying and sequencing dsRNA

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