Metagenomic Mining of Enzyme Diversity

Distaso, Marco A.; Tran, Hai; Ferrer, Manuel; Golyshin, Peter N.

doi:10.1007/978-3-319-31421-1_216-1

Cited by 1 publication

(2 citation statements)

References 122 publications

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“…Subsequent analysis using sequence data exploration platforms such as those offered by the Joint Genome Institute [ 74 ] and functional prediction software such as HMMER [ 75 ] are utilised to mine for known motifs and predict putative protein function based on sequence homology. However, sequence homology-based approaches are limited to identifying known motifs, and therefore they cannot identify novel activity types [ 76 ].…”

Section: Discovery Of Extremophilic Enzymes For Polyester Recyclingmentioning

confidence: 99%

“…This approach was used by many groups with great success leading to a trove of enzyme discoveries [ 51 , 87 , 90–93 ]. There are certain limitations to this approach, including a narrow range of hosts, suboptimal protein expression, and reliance on general substrates [ 76 , 94 ]; moreover, the only recent development of metagenome screens directly assaying for plastic biodegradation activity [ 95 ] means that more time will be required for such screens to uncover novel classes of polymer-degrading enzymes.…”

Section: Discovery Of Extremophilic Enzymes For Polyester Recyclingmentioning

confidence: 99%

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Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling

Williams

Khusnutdinova

et al. 2023

Essays in Biochemistry

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The steady growth in industrial production of synthetic plastics and their limited recycling have resulted in severe environmental pollution and contribute to global warming and oil depletion. Currently, there is an urgent need to develop efficient plastic recycling technologies to prevent further environmental pollution and recover chemical feedstocks for polymer re-synthesis and upcycling in a circular economy. Enzymatic depolymerization of synthetic polyesters by microbial carboxylesterases provides an attractive addition to existing mechanical and chemical recycling technologies due to enzyme specificity, low energy consumption, and mild reaction conditions. Carboxylesterases constitute a diverse group of serine-dependent hydrolases catalysing the cleavage and formation of ester bonds. However, the stability and hydrolytic activity of identified natural esterases towards synthetic polyesters are usually insufficient for applications in industrial polyester recycling. This necessitates further efforts on the discovery of robust enzymes, as well as protein engineering of natural enzymes for enhanced activity and stability. In this essay, we discuss the current knowledge of microbial carboxylesterases that degrade polyesters (polyesterases) with focus on polyethylene terephthalate (PET), which is one of the five major synthetic polymers. Then, we briefly review the recent progress in the discovery and protein engineering of microbial polyesterases, as well as developing enzyme cocktails and secreted protein expression for applications in the depolymerisation of polyester blends and mixed plastics. Future research aimed at the discovery of novel polyesterases from extreme environments and protein engineering for improved performance will aid developing efficient polyester recycling technologies for the circular plastics economy.

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Section: Discovery Of Extremophilic Enzymes For Polyester Recyclingmentioning

confidence: 99%

Section: Discovery Of Extremophilic Enzymes For Polyester Recyclingmentioning

confidence: 99%

Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling

Williams

Khusnutdinova

et al. 2023

Essays in Biochemistry

View full text Add to dashboard Cite

show abstract

Metagenomic Mining of Enzyme Diversity

Cited by 1 publication

References 122 publications

Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling

Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling

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