2022
DOI: 10.3389/ti.2022.10265
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Metagenomic Next-Generation Sequencing for Diagnosing Infections in Lung Transplant Recipients: A Retrospective Study

Abstract: Background: Accurate identification of pathogens is essential for the diagnosis and control of infections. We aimed to compare the diagnostic performance of metagenomic next-generation sequencing (mNGS) and conventional detection methods (CDM) in lung transplant recipients (LTRs).Methods: We retrospectively analyzed 107 LTRs with suspected infection of pulmonary, blood, central nervous system or chest wall between March 2018 and November 2020. Bronchoalveolar lavage fluid and other body fluids were subject to … Show more

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Cited by 13 publications
(9 citation statements)
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“…Additionally, DSA should be present for AMR. Pulmonary infection was diagnosed based on our previous studies [27,28]. There were no pathogens in the AR or CLAD groups.…”
Section: Histopathology From Lung Biopsy Tissuementioning
confidence: 99%
“…Additionally, DSA should be present for AMR. Pulmonary infection was diagnosed based on our previous studies [27,28]. There were no pathogens in the AR or CLAD groups.…”
Section: Histopathology From Lung Biopsy Tissuementioning
confidence: 99%
“…A key issue with respect to diagnosis is the need to develop sensitive and reliable assays [ 50 ]. Of note that nucleic acid tests and metagenomic next-generation sequencing is being explored and has been recently highlighted for fungi diagnosis in the setting of donor-derived transmission of scedosporiosis [ 51 , 52 ].…”
Section: Cryptococcus and Moldsmentioning
confidence: 99%
“…Potential allograft infection was indicated by the detection of pathogens, including bacteria, fungi, and viruses, in BALF detected by mNGS or conventional methodsas described in our previous studies [ 20 , 21 ].…”
Section: Methodsmentioning
confidence: 99%
“…An adequate volume of plasma (2 mL) was assayed by using a clinical-grade NGS dd-cfDNA system at a certified laboratory (AlloDx Biotech, Co., Ltd). A total of 6200 human single nucleotide polymorphism loci were enriched through liquid hybridization [ 20 ]. Briefly, 8 mL of blood was drawn into a tube for the collection of cfDNA (Streck, Omaha, NE).…”
Section: Methodsmentioning
confidence: 99%