Metagenomic Next-Generation Sequencing for the Diagnosis of Neonatal Infectious Diseases

Chen, Lu; Zhao, Yashuang; Wei, Jiakai; Huang, Wendi; Ma, Ying; Yang, Xiaoli; Liu, Yang; Wang, Jing; Xia, Han; Lou, Zheng

doi:10.1128/spectrum.01195-22

Cited by 10 publications

(10 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accurate diagnosis based on pathogen detection is crucial for patient care. Traditional culture-based detection methods often fail to identify uncultivatable or fastidious pathogens and are susceptible to empirical antibiotic treatment ( 7 ). PCR assays can be a useful tool for detecting pathogens that are difficult or impossible to culture.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic-based pathogen surveillance for children with severe pneumonia in pediatric intensive care unit

Wang²,

Yao

et al. 2023

Front. Public Health

Self Cite

View full text Add to dashboard Cite

BackgroundPneumonia is a significant cause of morbidity and mortality in children. Metagenomic next-generation sequencing (mNGS) has the potential to assess the landscape of pathogens responsible for severe pulmonary infection.MethodsBronchoalveolar lavage fluid (BALF) samples of 262 children with suspected pulmonary infections were collected from April 2019 to October 2021 in the Pediatric Intensive Care Unit (PICU) of Guangdong Women and Children Hospital. Both mNGS and conventional tests were utilized for pathogen detection.ResultsA total of 80 underlying pathogens were identified using both mNGS and conventional tests. Respiratory syncytial virus (RSV), Staphylococcus aureus and rhinovirus were the most frequently detected pathogens in this cohort. The incidence rate of co-infection was high (58.96%, 148/251), with bacterial-viral agents most co-detected. RSV was the main pathogen in children younger than 6 months of age, and was also commonly found in older pediatric patients. Rhinovirus was prevalent in children older than 6 months. Adenovirus and Mycoplasma pneumoniae were more prevalent in children older than 3 years than in other age groups. Pneumocystis jirovecii was detected in nearly 15% of children younger than 6 months. Besides, influenza virus and adenovirus were rarely found in 2020 and 2021.ConclusionsOur study highlights the importance of using advanced diagnostic techniques like mNGS to improve our understanding of the microbial epidemiology of severe pneumonia in pediatric patients.

show abstract

Section: Introductionmentioning

confidence: 99%

Metagenomic-based pathogen surveillance for children with severe pneumonia in pediatric intensive care unit

Wang²,

Yao

et al. 2023

Front. Public Health

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chen et al. also exhibited the important role of repeated mNGS tests in diagnosing and managing Escherichia coli infection of neonates ( Chen et al., 2022 ). The findings of our study provide more evidences to further emphasize the importance of mNGS in diagnosing, managing, and ruling out infections, and an era of more rapid, independent, and impartial diagnosis of infections in patients after HSCT can be expected.…”

Section: Discussionmentioning

confidence: 99%

Combination of metagenomic next-generation sequencing and conventional tests unraveled pathogen profiles in infected patients undergoing allogeneic hematopoietic stem cell transplantation in Jilin Province of China

Zou,

Gao,

Liu

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

BackgroundInfection is the main cause of death for patients after allogeneic hematopoietic stem cell transplantation (HSCT). However, pathogen profiles still have not been reported in detail due to their heterogeneity caused by geographic region.ObjectiveTo evaluate the performance of metagenomic next-generation sequencing (mNGS) and summarize regional pathogen profiles of infected patients after HSCT.MethodsFrom February 2021 to August 2022, 64 patients, admitted to the Department of Hematology of The First Hospital of Jilin University for HSCT and diagnosed as suspected infections, were retrospectively enrolled.ResultsA total of 38 patients were diagnosed as having infections, including bloodstream (n =17), pulmonary (n =16), central nervous system (CNS) (n =4), and chest (n =1) infections. Human betaherpesvirus 5 (CMV) was the most common pathogen in both bloodstream (n =10) and pulmonary (n =8) infections, while CNS (n =2) and chest (n =1) infections were mainly caused by Human gammaherpesvirus 4 (EBV). For bloodstream infection, Mycobacterium tuberculosis complex (n =3), Staphylococcus epidermidis (n =1), and Candida tropicalis (n =1) were also diagnosed as causative pathogens. Furthermore, mNGS combined with conventional tests can identify more causative pathogens with high sensitivity of 82.9% (95% CI 70.4-95.3%), and the total coincidence rate can reach up to 76.7% (95% CI 64.1-89.4%).ConclusionsOur findings emphasized the importance of mNGS in diagnosing, managing, and ruling out infections, and an era of more rapid, independent, and impartial diagnosis of infections after HSCT can be expected.

show abstract

“…Chen et al. also found that the best timing for mNGS detection in neonatal infections ranged from 1 to 3 days, rather than 0 days, after the start of continuous anti-microbial therapy ( Chen et al., 2022 ). Given the inevitability and indispensability of empirical therapy, most children received empirical therapy after the onset of infection ( Nascimento-Carvalho and Nascimento-Carvalho, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

High specificity of metagenomic next-generation sequencing using protected bronchial brushing sample in diagnosing pneumonia in children

Liu

Wang

Zhou

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

BackgroundLower respiratory tract infections are the leading cause of morbidity and mortality in children worldwide. Timely and accurate pathogen detection is crucial for proper clinical diagnosis and therapeutic strategies. The low detection efficiency of conventional methods and low specificity using respiratory samples seriously hindered the accurate detection of pathogens.MethodsIn this study, we retrospectively enrolled 1,032 children to evaluate the performance of metagenomics next-generation sequencing (mNGS) using bronchoalveolar lavage fluid (BALF) sample and protected bronchial brushing (BB) sample in diagnosing pneumonia in children. In addition, conventional tests (CTs) were also performed.ResultsThe specificity of BB mNGS [67.3% (95% CI 58.6%–75.9%)] was significantly higher than that of BALF mNGS [38.5% (95% CI 12.0%–64.9%)]. The total coincidence rate of BB mNGS [77.6% (95% CI 74.8%–80.5%)] was slightly higher than that of BALF mNGS [76.5% (95% CI 68.8%–84.1%)] and CTs [38.5% (95% CI 35.2%–41.9%)]. During the epidemics of Mycoplasma pneumoniae, the detection rate of M. pneumoniae in the >6-year group (81.8%) was higher than that in the 3–6-year (78.9%) and <3-year groups (21.5%). The highest detection rates of bacteria, fungi, and viruses were found in the <3-year, >6-year, and 3–6-year groups, respectively. mNGS detection should be performed at the duration of 5–7 days after the start of continuous anti-microbial therapy or at the duration of 6–9 days from onset to mNGS test.ConclusionsThis is the first report to evaluate the performance of BB mNGS in diagnosing pulmonary infections in children on a large scale. Based on our findings, extensive application of BB mNGS could be expected.

show abstract

Metagenomic Next-Generation Sequencing for the Diagnosis of Neonatal Infectious Diseases

Cited by 10 publications

References 54 publications

Metagenomic-based pathogen surveillance for children with severe pneumonia in pediatric intensive care unit

Metagenomic-based pathogen surveillance for children with severe pneumonia in pediatric intensive care unit

Combination of metagenomic next-generation sequencing and conventional tests unraveled pathogen profiles in infected patients undergoing allogeneic hematopoietic stem cell transplantation in Jilin Province of China

High specificity of metagenomic next-generation sequencing using protected bronchial brushing sample in diagnosing pneumonia in children

Contact Info

Product

Resources

About