Metagenomic Next-Generation Sequencing to Investigate Infectious Endophthalmitis of Brucella: A Case Report

Xi, Huiyu; Zhang, Lishuai; Xu, Bo; Liu, Haiyang; Li, Suyan

doi:10.3389/fmed.2022.847143

Cited by 9 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Afterwards, with the advent of an unbiased sequencing technique, the NGS of 16S rDNA amplicons and later the application of shotgun metagenomics, a more advanced and prompt method has been proposed for the detection of insidious pathogens in endophthalmitis. All pathogens, even those unculturable can be detected, without being limited by the starting primers [50][51] . Although NGS has the advantage of high throughput, its short-read characteristics render it difficult to identify repeats in gene sequences, followed by several other defects [52][53][54] .…”

Section: Molecular Diagnostic Techniques Of Endophthalmitismentioning

confidence: 99%

Nanopore techniques as a potent tool in the diagnosis and treatment of endophthalmitis: a literature review

Li¹,

Jin²

2022

Int J Ophthalmol

View full text Add to dashboard Cite

Endophthalmitis is a serious ophthalmic disease characterized by changes in the eye’s posterior segment, such as hypopyon and intraocular inflammation, vitritis being a hallmark. Infection-caused endophthalmitis can lead to irreversible vision loss, accompanied by eye pain or eye distention, and in the most severe cases the removal of the eyeball. Microorganisms such as bacteria, fungi, viruses, and parasites typically account for the disease and the entry pathways of the microbial can be divided into either endogenous or exogenous approaches, according to the origin of the etiological agents. Exogenous endophthalmitis can be derived from various occasions (such as post-operative complications or trauma) while endogenous endophthalmitis results from the bloodstream which carries pathogens to the eye. This review aims to summarize the application of new technology in pathogen identification of endophthalmitis so as to prevent the disease and better guide clinical diagnosis and treatment.

show abstract

Section: Molecular Diagnostic Techniques Of Endophthalmitismentioning

confidence: 99%

Nanopore techniques as a potent tool in the diagnosis and treatment of endophthalmitis: a literature review

Li¹,

Jin²

2022

Int J Ophthalmol

View full text Add to dashboard Cite

show abstract

“…In previous studies, the causative bacterial species varied according to the endophthalmitis type. Notably, bacterial agents could not be identified in numerous cases, highlighting diagnostic challenges 22. Metagenomic analysis makes it possible to detect pathogenic bacteria at the species level 23 24.…”

Section: Discussionmentioning

confidence: 99%

“…Notably, bacterial agents could not be identified in numerous cases, highlighting diagnostic challenges. 22 Metagenomic analysis makes it possible to detect pathogenic bacteria at the species level. 23 24 Previous reports have shown that a 16S rRNA metagenomic analysis encompasses the whole genomic DNA present in the sample, leading to potential issues with contamination.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of 16S rRNA metagenomic sequencing in the diagnosis and understanding of bacterial endophthalmitis

Asao,

Hashida,

Maruyama

et al. 2023

BMJ Open Ophth

View full text Add to dashboard Cite

ObjectiveTo evaluate the usefulness of metagenomic analysis in the search for causative organisms of bacterial endophthalmitis.Methods and analysisTwenty-one consecutive treatment-naïve patients (13 men and 8 women; mean age, 60.8±19.8 years) with suspected endophthalmitis were recruited. Vitrectomy was performed to diagnose and treat endophthalmitis. Bacterial culture and metagenomic analysis of the vitreous body were performed. Extracted DNA was analysed using 16S rRNA sequences, and libraries were sequenced on an Illumina MiSeq sequencer. To compare the bacterial composition in each case, α and β diversities were determined.ResultsPatients were categorised into three groups: endophthalmitis cases with matching predominant organisms according to metagenomic analysis and bacterial culture, those with negative results for bacterial culture and those with negative results in both cases. In 7 of 15 culture-negative cases, results from metagenomic analysis could detect pathogens. The diversity of bacterial populations was significantly lower in the group with positive results for predominant bacteria according to culture and metagenomic analysis. All patients with uveitis were included in the group for which the causative pathogen could not be determined by culture or metagenomic analysis. The structures of bacterial populations significantly differed between the positive and negative groups by culture and metagenomic analysis.ConclusionsMetagenomic analysis could be useful for prompt detection of causative pathogens, for precise diagnosis of infection, and as a marker of inflammation processes such as uveitis.

show abstract

“…Metagenomic analysis requires proper interpretation of the data collected, and many issues remain, including diagnostic thresholds and contamination. It was necessary to remove known background contaminants from the data of potential pathogens for each subject [30]. The big issues are the high cost and costly maintenance.…”

Section: Metagenomic High-throughput Next-generation Sequencing (Mngs)mentioning

confidence: 99%

Role of Recent PCR Tests for Infectious Ocular Diseases: From Laboratory-Based Studies to the Clinic

Sugita¹,

Takase

Nakano

2023

IJMS

View full text Add to dashboard Cite

Infectious uveitis is a vision-threatening condition that requires prompt clinical diagnosis and proper treatment. However, rapid and proper diagnosis in infectious uveitis remains challenging. Several examination tests, including polymerase chain reaction (PCR) tests, are transitioning from laboratory-based basic research-level tests to bedside clinical tests, and recently tests have changed to where they can be performed right next to clinicians. In this review, we introduce an updated overview of recent studies that are representative of the current trends in clinical microbiological techniques including PCR tests for infectious uveitis.

show abstract

Metagenomic Next-Generation Sequencing to Investigate Infectious Endophthalmitis of Brucella: A Case Report

Cited by 9 publications

References 28 publications

Nanopore techniques as a potent tool in the diagnosis and treatment of endophthalmitis: a literature review

Nanopore techniques as a potent tool in the diagnosis and treatment of endophthalmitis: a literature review

Comparative evaluation of 16S rRNA metagenomic sequencing in the diagnosis and understanding of bacterial endophthalmitis

Role of Recent PCR Tests for Infectious Ocular Diseases: From Laboratory-Based Studies to the Clinic

Contact Info

Product

Resources

About