Metagenomics in pooled plasma, with identification of potential emerging infectious pathogens

Xu, Min; Yang, Yan; Ya, Zhou; Liu, Zhiyang; Liu, Yu; He, Miao

doi:10.1111/trf.14455

Cited by 8 publications

(14 citation statements)

References 19 publications

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“…The blood collection and supply system in China does not perform routine screening for toxoplasmosis: however, whether Toxoplasma gondii detection should be performed for certain blood recipient populations is worth consideration. Furthermore, DNA fragments of pathogens that are considered threats blood transfusion safety in Europe and America 8 , including as P. falciparum and L. infantum, were discovered in this study. Considering that malaria and Leishmania infection are currently resurging 27 , 28 and that Luzhou and the surrounding region are within the endemic area, the blood collection and supply system should enhance their surveillance of these parasites in donated blood samples.…”

Section: Discussionmentioning

confidence: 68%

“…Recently, emerging/re-emerging infectious diseases have become important threats to human life and safety 1 , 2 , 8 . More than 12% of human infectious diseases are categorized as emerging/re-emerging infectious diseases 9 .…”

Section: Introductionmentioning

confidence: 99%

“…A wide range of emerging/re-emerging infectious pathogens exist, the classification of which is complicated by the high degree of genetic variation. Therefore, routine analytical methods cannot completely detect and identify emerging/re-emerging pathogens 8 , 10 . Human cytomegalovirus (HCMV, also known as human herpesvirus 5) is a ubiquitous β herpes virus 11 , 12 that frequently causes various infections in individuals.…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic analysis and identification of emerging pathogens in blood from healthy donors

Gao

et al. 2020

Sci Rep

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Emerging infectious pathogens that threaten blood transfusions are known to be present in blood samples from healthy/qualified donors. The objective of this study was to investigate the microbiome of blood from healthy donors from the Luzhou area in southwestern China. Potential pathogens and cytomegalovirus (CMV) infection in the donor blood were identified. Total plasma nucleic acids were extracted from one pool of 5734 samples and were constructed for metagenomics analysis using Illumina sequencing. The microbiome and potential emerging/re-emerging pathogens were identified using bioinformatics analysis. Moreover, CMV antigen was measured via an enzyme-linked immunosorbent assay, and the CMV DNA level was assessed by quantitative RT-PCR. A total of 132 bacterial reads, 65 viral reads and 165 parasitic reads were obtained. The most frequent bacterium was Escherichia coli (95/132, 72%) with 95 reads in 132 bacterial reads, and the most prevalent parasite was Toxoplasma gondii (131/165, 79%). Among the viruses, cytomegalovirus (44/65, 68%) accounted for the highest frequency, followed by Hepatitis E Virus (10/65, 15%). Moreover, the positive rate of CMV-IgG was 46.25% (2652/5734), and the positive rate of CMV-IgM was 5.82% (334/5734). The positive rate of dual positive (IgG+ and IgM+) CMV was 0.07% (4/5734). Twenty-one (0.37%) specimens from 5734 donated blood samples were positive for CMV DNA. The CMV DNA levels ranged from 7.56 × 102 to 3.58 × 103 copies/mL. The current study elucidated the microbiome structure in blood from healthy/qualified donors in the Luzhou area and identified emerging/re-emerging pathogens. This preliminary study contributes to information regarding blood transfusion safety in China.

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Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metagenomic analysis and identification of emerging pathogens in blood from healthy donors

Gao

et al. 2020

Sci Rep

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“…Human papillomavirus type 27 and Merkel cell polyomavirus present on the skin were also detected . Thirteen species of bacteria and two parasites that might potentially threaten blood safety were identified along with six nonpathogenic viruses from pooled plasma of manufacturers in China . Human pegivirus A, human papillomavirus and gemycircularvirus were identified in plasma pools from USA and Europe .…”

Section: Introductionmentioning

confidence: 99%

“…4 Thirteen species of bacteria and two parasites that might potentially threaten blood safety were identified along with six nonpathogenic viruses from pooled plasma of manufacturers in China. 5 Human pegivirus A, human papillomavirus and gemycircularvirus were identified in plasma pools from USA and Europe. 6 Therefore, MGA appears a promising tool for detecting common and less frequent infectious pathogens in blood for transfusion.…”

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confidence: 99%

Metagenomic analysis of potential pathogens from blood donors in Guangzhou, China

Gao

Xiao

et al. 2019

Transfusion Medicine

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Objectives This study aimed to identify the emerging/reemerging pathogens in blood donation samples. Background A metagenomic analysis has previously been used to look for pathogens but in this study, the relationship with aminotransferase (ALT) is described. Methods/Materials Excluding samples reactive to hepatitis B virus, hepatitis C virus, human immunodeficiency syndrome virus or syphilis and plasma samples were stratified into three groups of ALT levels (IU/L): A ≤ 50, B 51 to 69 and C ≥ 70, respectively. Each group was mixed in a pool of 100 samples, from which DNA and cDNA libraries were established for next generation sequencing and analysis. Pathogens of interest were identified by immunoassays, nested‐polymerase chain reaction, phylogenetic analysis and pathogen detection in follow‐up donors. Results Several new or reemerging transfusion‐transmitted pathogens were identified; Streptococcus suis, Babesia species and Toxoplasma gondii were found in the three ALT groups, Epstein‐Barr virus (EBV) only in group C. Ten S. suis nucleic acid positive samples were detected, all closely phylogenetically related to reference strains. A donor in group A carried both S. suis genome and specific IgM in follow‐up samples. This strain was identified as nontoxic S. suis. Five samples contained a short fragment of Babesia species SpeI‐AvaI gene, while T. gondii was identified in 20 samples as a short fragment of 18S rDNA gene. In group C, two samples contained EBV genome. Conclusions Blood donations that contained S. suis, Babesia species and T. gondii sequences might represent potential transfusion risks. EBV, a potential cause of elevated ALT, was detected. Metagenomic analysis might be a useful technology for monitoring blood safety.

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Clinical metagenomics for infection diagnosis

Salais

Filkins

2019

Genomic and Precision Medicine

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Metagenomics in pooled plasma, with identification of potential emerging infectious pathogens

Cited by 8 publications

References 19 publications

Metagenomic analysis and identification of emerging pathogens in blood from healthy donors

Metagenomic analysis and identification of emerging pathogens in blood from healthy donors

Metagenomic analysis of potential pathogens from blood donors in Guangzhou, China

Clinical metagenomics for infection diagnosis

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