Metagenomics: Is it a powerful tool to obtain lipases for application in biocatalysis?

Enzyme production ofd-gluconic acid and glucose oxidase: successful tales of cascade reactions

“…[89][90][91][92][93] However, due to their biological origin, they have some features that are far from ideal for industrial requirements. 94 Current developments in all areas related to enzyme biocatalyst design (e.g., metagenomics, [95][96][97][98] directed evolution, [99][100][101] chemical modification, 102 and immobilization [103][104][105][106][107][108] ) can solve these problems. For example, several new strategies have been published, such as the design of plurizymes (enzymes bearing a natural and an artificially designed active center), the design of irreversible inhibitors directed to one of these active center bearing a metal catalytic center and the use of modified plurizymes (bearing a free active center while the other active center presented the inhibitor with a metal catalysts) in a cascade reaction.…”

Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry

“…Heterologous expression has also been the choice of many studies of recombinant lipases produced by uncultured microorganisms from extreme environmental conditions. Their lipases encoding genes can be isolated and expressed in heterologous expression systems by the construction of functional metagenomics libraries [50][51][52]. Functional metagenomics has the advantage of not requiring either individual genome sequencing or the cultivation of unknown producer microorganisms.…”

“…Functional metagenomics has the advantage of not requiring either individual genome sequencing or the cultivation of unknown producer microorganisms. For more metagenomics studies of lipases with biotechnological potential, Almeida and collaborators [52] provide an interesting review.…”

Sensitive and Rapid Phenotyping of Microbes With Soluble Methane Monooxygenase Using a Droplet-Based Assay

“…All compartments are miniaturized bioreactors with precisely controlled reaction volumes, cell numbers, reagent concentrations, and incubation times (Joensson and Andersson Svahn, 2012). Therefore, dropletbased microfluidics has been successfully used as a screening platform with broad applications, such as for the directed evolution of enzymes, identification of novel metagenomic enzymes, and production of extracellular chemicals (Agresti et al, 2010;Hosokawa et al, 2015;Kaushik et al, 2017;Siedler et al, 2017;Giuffrida et al, 2018;de Almeida et al, 2019). It is possible to extend this technology to methanotroph phenotyping, which has not been performed before.…”